Restriction map for
pF1KE2503
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaHI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NsiI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PshBI PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 341 GTCGAC GT^[AC][GT]AC
AclI 572 AACGTT AA^CGTT
AcsI 1603 AAATTC [GA]^AATT[CT]
AflIII 726 ACACGT A^C[GA][CT]GT
ApaI 806 GGGCCC GGGCC^C
ApoI 1603 AAATTC [GA]^AATT[CT]
BanI 178 GGTGCC G^G[CT][GA]CC
BbrPI 727 CACGTG CAC^GTG
BlpI 934 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 934 GCTGAGC GC^T[ACGT]AGC
BsaAI 727 CACGTG [CT]AC^GT[GA]
BsaBI 877 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 605 GGTCTC GGTCTC(1/5)
BsaWI 1415 ACCGGA [AT]^CCGG[AT]
BsmBI 1267 GAGACG CGTCTC(1/5)
BsrFI 559 GCCGGC [GA]^CCGG[CT]
BssSI 1195 CACGAG CACGAG(-5/-1)
CelII 934 GCTGAGC GC^T[ACGT]AGC
Cfr10I 559 GCCGGC [GA]^CCGG[CT]
DraII 805 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 1068 CCGTGG C^C[GA][CT]GG
EcoO109I 805 AGGGCCC [GA]G^G[ACGT]CC[CT]
FseI 558 GGCCGGCC GGCCGG^CC
HincII 341 GTCGAC GT[CT]^[GA]AC
HindII 341 GTCGAC GT[CT]^[GA]AC
MamI 877 GATACGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 752 TTAA T^TAA
MslI 62 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 559 GCCGGC GCC^GGC
NgoMIV 559 GCCGGC G^CCGGC
NruI 1089 TCGCGA TCG^CGA
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
Psp1406I 572 AACGTT AA^CGTT
SalI 341 GTCGAC G^TCGAC
SphI 276 GCATGC GCATG^C
TfiI 1403 GATTC G^A[AT]TC
Tru9I 752 TTAA T^TAA
Tsp509I 1604 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 281 CGCG CG^CG
1090 CGCG
AfaI 1413 GTAC GT^AC
1441 GTAC
AlwI 96 GGATC GGATC(4/5)
552 GGATC
BcnI 948 CCCGG CC^[GC]GG
1489 CCGGG
BfaI 451 CTAG C^TAG
1334 CTAG
BsiEI 561 CGGCCG CG[GA][CT]^CG
1280 CGGCCG
BstAPI 708 GCAGATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1838 GCAGCAACTGC
BstUI 281 CGCG CG^CG
1090 CGCG
BstXI 548 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1715 CCAACAGCGTGG
BstYI 96 GGATCT [GA]^GATC[CT]
552 GGATCT
CfoI 280 GCGC GCG^C
1208 GCGC
EagI 561 CGGCCG C^GGCCG
1280 CGGCCG
EarI 541 GAAGAG CTCTTC(1/4)
1143 GAAGAG
EclXI 561 CGGCCG C^GGCCG
1280 CGGCCG
Eco52I 561 CGGCCG C^GGCCG
1280 CGGCCG
EcoT14I 548 CCAAGG C^C[AT][AT]GG
1797 CCTTGG
HhaI 280 GCGC GCG^C
1208 GCGC
HinP1I 280 GCGC G^CGC
1208 GCGC
Ksp632I 541 GAAGAG CTCTTC(1/4)
1143 GAAGAG
MaeI 451 CTAG C^TAG
1334 CTAG
MflI 96 GGATCT [GA]^GATC[CT]
552 GGATCT
MvnI 281 CGCG CG^CG
1090 CGCG
NciI 948 CCCGG CC^[GC]GG
1489 CCGGG
NspI 276 GCATGC [GA]CATG^[CT]
1380 GCATGT
PaeR7I 321 CTCGAG C^TCGAG
654 CTCGAG
PflMI 7 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCAGGTGATGG
RsaI 1413 GTAC GT^AC
1441 GTAC
SacI 1021 GAGCTC GAGCT^C
1666 GAGCTC
StyI 548 CCAAGG C^C[AT][AT]GG
1797 CCTTGG
Van91I 7 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCAGGTGATGG
XcmI 1500 CCATCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1855 CCATGCTTTTGCTGG
XhoI 321 CTCGAG C^TCGAG
654 CTCGAG
XhoII 96 GGATCT [GA]^GATC[CT]
552 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwNI AocI AspHI
AvaI AvaII BanII BbvI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
EaeI Eco81I EcoNI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI Tsp45I TspRI TthHB8I