Restriction map for
pF1KSDA0845
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 25 enzymes) #
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Aor51HI 1034 AGCGCT AGC^GCT
Asp700I 1084 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 538 CACGTG CAC^GTG
BsaAI 538 CACGTG [CT]AC^GT[GA]
BsaI 1387 GAGACC GGTCTC(1/5)
BsaWI 2871 ACCGGA [AT]^CCGG[AT]
BsgI 847 GTGCAG GTGCAG(16/14)
BsiEI 315 CGACCG CG[GA][CT]^CG
BsmBI 772 GAGACG CGTCTC(1/5)
BsrBI 414 CCGCTC CCGCTC(-3/-3)
BsrDI 1068 CATTGC GCAATG(2/0)
BssSI 144 CTCGTG CACGAG(-5/-1)
BstAPI 660 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 986 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 150 GACACGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 195 CCGTGG C^C[GA][CT]GG
Eco47III 1034 AGCGCT AGC^GCT
MslI 1305 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 81 GCTAGC G^CTAGC
PaeR7I 523 CTCGAG C^TCGAG
PmaCI 538 CACGTG CAC^GTG
PmlI 538 CACGTG CAC^GTG
SacI 165 GAGCTC GAGCT^C
XhoI 523 CTCGAG C^TCGAG
XmnI 1084 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 156 GACGTC GACGT^C
801 GACGTC
AcsI 1283 AAATTC [GA]^AATT[CT]
2356 AAATTC
AhdI 796 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
892 GACCGACTGTC
AlwI 732 GATCC GGATC(4/5)
2457 GATCC
ApoI 1283 AAATTC [GA]^AATT[CT]
2356 AAATTC
AspEI 796 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
892 GACCGACTGTC
BalI 272 TGGCCA TGG^CCA
2801 TGGCCA
BbsI 2770 GAAGAC GAAGAC(2/6)
3055 GAAGAC
BpuAI 2770 GAAGAC GAAGAC(2/6)
3055 GAAGAC
BstF5I 1529 CATCC GGATG(2/0)
2433 GGATG
BstYI 731 AGATCC [GA]^GATC[CT]
2456 AGATCC
Eam1105I 796 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
892 GACCGACTGTC
FokI 1529 CATCC GGATG(9/13)
2433 GGATG
MflI 731 AGATCC [GA]^GATC[CT]
2456 AGATCC
MluNI 272 TGGCCA TGG^CCA
2801 TGGCCA
MscI 272 TGGCCA TGG^CCA
2801 TGGCCA
NlaIII 55 CATG CATG^
261 CATG
PpuMI 1157 AGGACCT [GA]G^G[AT]CC[CT]
2341 AGGTCCC
SmlI 213 CTCAAG C^T[CT][GA]AG
523 CTCGAG
TfiI 819 GATTC G^A[AT]TC
2677 GAATC
XhoII 731 AGATCC [GA]^GATC[CT]
2456 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI AspHI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstNI BstUI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI