Restriction map for
pF1KSDA0844
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspMI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 490 AAATTC [GA]^AATT[CT]
AflIII 477 ACATGT A^C[GA][CT]GT
ApaI 131 GGGCCC GGGCC^C
ApoI 490 AAATTC [GA]^AATT[CT]
AspHI 1141 GAGCTC G[AT]GC[AT]^C
AvaI 84 CCCGAG C^[CT]CG[GA]G
BanI 971 GGCACC G^G[CT][GA]CC
BbrPI 919 CACGTG CAC^GTG
BfaI 217 CTAG C^TAG
BglI 1157 GCCGCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 919 CACGTG [CT]AC^GT[GA]
BsiEI 427 CGGTCG CG[GA][CT]^CG
BsiHKAI 1141 GAGCTC G[AT]GC[AT]^C
BsmBI 636 GAGACG CGTCTC(1/5)
BsmI 978 GCATTC GAATGC(1/-1)
BsoBI 84 CCCGAG C^[CT]CG[GA]G
Bsp1407I 302 TGTACA T^GTACA
BspLU11I 477 ACATGT A^CATGT
BsrBI 643 GAGCGG CCGCTC(-3/-3)
BsrGI 302 TGTACA T^GTACA
BsrI 849 ACTGG ACTGG(1/-1)
BstXI 551 CCAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 531 GAAGAG CTCTTC(1/4)
EcoRV 29 GATATC GAT^ATC
HincII 295 GTTAAC GT[CT]^[GA]AC
HindII 295 GTTAAC GT[CT]^[GA]AC
HpaI 295 GTTAAC GTT^AAC
HphI 548 TCACC GGTGA(8/7)
Ksp632I 531 GAAGAG CTCTTC(1/4)
MaeI 217 CTAG C^TAG
MslI 387 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 1157 GCCGCAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 392 CCATGG C^CATGG
PflMI 762 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 919 CACGTG CAC^GTG
PmlI 919 CACGTG CAC^GTG
PpuMI 432 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1119 CTGCAG CTGCA^G
SacI 1141 GAGCTC GAGCT^C
ScaI 869 AGTACT AGT^ACT
SfcI 1119 CTGCAG C^T[GA][CT]AG
SphI 1067 GCATGC GCATG^C
SspBI 302 TGTACA T^GTACA
Van91I 762 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 882 CCAGCAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AspI 214 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
423 GACACGGTC
BanII 131 GGGCCC G[GA]GC[CT]^C
1141 GAGCTC
BcnI 244 CCGGG CC^[GC]GG
806 CCCGG
BpmI 811 CTCCAG CTGGAG(16/14)
139 CTGGAG
BsaI 95 GAGACC GGTCTC(1/5)
1112 GAGACC
BsgI 376 GTGCAG GTGCAG(16/14)
604 GTGCAG
BsmFI 421 GGGAC GGGAC(10/14)
1008 GTCCC
DpnII 907 GATC ^GATC
1000 GATC
DraII 130 AGGGCCC [GA]G^G[ACGT]CC[CT]
432 GGGTCCT
DsaI 392 CCATGG C^C[GA][CT]GG
674 CCGTGG
EaeI 671 TGGCCG [CT]^GGCC[GA]
1155 TGGCCG
EcoNI 938 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1038 CCTGAAAAAGG
EcoO109I 130 AGGGCCC [GA]G^G[ACGT]CC[CT]
432 GGGTCCT
HapII 244 CCGG C^CGG
807 CCGG
HindIII 114 AAGCTT A^AGCTT
461 AAGCTT
HpaII 244 CCGG C^CGG
807 CCGG
MboI 907 GATC ^GATC
1000 GATC
MseI 296 TTAA T^TAA
652 TTAA
MspI 244 CCGG C^CGG
807 CCGG
NciI 244 CCGGG CC^[GC]GG
806 CCCGG
NdeII 907 GATC ^GATC
1000 GATC
NspI 477 ACATGT [GA]CATG^[CT]
1067 GCATGC
PflFI 214 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
423 GACACGGTC
PvuII 607 CAGCTG CAG^CTG
779 CAGCTG
Sau3AI 907 GATC ^GATC
1000 GATC
StuI 361 AGGCCT AGG^CCT
887 AGGCCT
TaqI 470 TCGA T^CGA
498 TCGA
TfiI 712 GAATC G^A[AT]TC
828 GAATC
Tru9I 296 TTAA T^TAA
652 TTAA
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
387 CACTG
Tth111I 214 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
423 GACACGGTC
TthHB8I 470 TCGA T^CGA
498 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
ItaI MaeII MaeIII MboII MnlI
MspA1I MvaI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SmlI StyI
TseI Tsp45I Tsp509I