Restriction map for
pF1KSDA0794
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspEI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PleI PmeI PpuMI PshAI
PshBI PstI PvuI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AclI 1349 AACGTT AA^CGTT
AcyI 24 GGCGTC G[GA]^CG[CT]C
AgeI 70 ACCGGT A^CCGGT
AlwI 1120 GATCC GGATC(4/5)
AspHI 287 GTGCTC G[AT]GC[AT]^C
BalI 480 TGGCCA TGG^CCA
BanI 725 GGCACC G^G[CT][GA]CC
BbrPI 1321 CACGTG CAC^GTG
BbsI 856 GAAGAC GAAGAC(2/6)
BglII 417 AGATCT A^GATCT
BpmI 1174 CTGGAG CTGGAG(16/14)
BpuAI 856 GAAGAC GAAGAC(2/6)
BsaAI 1321 CACGTG [CT]AC^GT[GA]
BsaHI 24 GGCGTC G[GA]^CG[CT]C
BsaWI 70 ACCGGT [AT]^CCGG[AT]
BsiHKAI 287 GTGCTC G[AT]GC[AT]^C
BsmFI 809 GTCCC GGGAC(10/14)
Bsp1407I 1452 TGTACA T^GTACA
BspDI 391 ATCGAT AT^CGAT
BspHI 621 TCATGA T^CATGA
BspMI 612 GCAGGT ACCTGC(4/8)
BsrBI 936 CCGCTC CCGCTC(-3/-3)
BsrDI 1412 CATTGC GCAATG(2/0)
BsrFI 70 ACCGGT [GA]^CCGG[CT]
BsrGI 1452 TGTACA T^GTACA
BstNI 1123 CCTGG CC^[AT]GG
BstYI 417 AGATCT [GA]^GATC[CT]
CfoI 34 GCGC GCG^C
Cfr10I 70 ACCGGT [GA]^CCGG[CT]
ClaI 391 ATCGAT AT^CGAT
EcoRII 1123 CCTGG ^CC[AT]GG
EcoT14I 1143 CCAAGG C^C[AT][AT]GG
EcoT22I 445 ATGCAT ATGCA^T
HaeII 33 GGCGCT [GA]GCGC^[CT]
HgaI 25 GCGTC GACGC(5/10)
HhaI 34 GCGC GCG^C
Hin1I 24 GGCGTC G[GA]^CG[CT]C
HinP1I 34 GCGC G^CGC
KspI 20 CCGCGG CCGC^GG
MflI 417 AGATCT [GA]^GATC[CT]
MluNI 480 TGGCCA TGG^CCA
MscI 480 TGGCCA TGG^CCA
MvaI 1123 CCTGG CC^[AT]GG
NdeI 97 CATATG CA^TATG
NheI 1305 GCTAGC G^CTAGC
NsiI 445 ATGCAT ATGCA^T
NspI 535 GCATGT [GA]CATG^[CT]
PflMI 277 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 70 ACCGGT A^CCGGT
PmaCI 1321 CACGTG CAC^GTG
PmlI 1321 CACGTG CAC^GTG
Psp1406I 1349 AACGTT AA^CGTT
RcaI 621 TCATGA T^CATGA
SacII 20 CCGCGG CCGC^GG
SapI 169 GAAGAGC GCTCTTC(1/4)
SmlI 1436 CTCAAG C^T[CT][GA]AG
SspBI 1452 TGTACA T^GTACA
SspI 583 AATATT AAT^ATT
StuI 1425 AGGCCT AGG^CCT
StyI 1143 CCAAGG C^C[AT][AT]GG
Van91I 277 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 417 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 21 CGCG CG^CG
553 CGCG
AfaI 179 GTAC GT^AC
1453 GTAC
Asp700I 223 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
349 GAAACTATTC
BanII 15 GGGCTC G[GA]GC[CT]^C
172 GAGCCC
BciVI 1336 GGATAC GTATCC(6/5)
1254 GTATCC
BcnI 333 CCGGG CC^[GC]GG
591 CCGGG
BsgI 308 CTGCAC GTGCAG(16/14)
1324 GTGCAG
BstF5I 150 GGATG GGATG(2/0)
792 GGATG
BstUI 21 CGCG CG^CG
553 CGCG
DdeI 939 CTCAG C^T[ACGT]AG
1100 CTGAG
EaeI 427 CGGCCA [CT]^GGCC[GA]
480 TGGCCA
FokI 150 GGATG GGATG(9/13)
792 GGATG
MaeIII 136 GTCAC ^GT[ACGT]AC
760 GTGAC
MslI 536 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
622 CATGACAGTG
MvnI 21 CGCG CG^CG
553 CGCG
NciI 333 CCGGG CC^[GC]GG
591 CCGGG
PvuII 1154 CAGCTG CAG^CTG
1240 CAGCTG
RsaI 179 GTAC GT^AC
1453 GTAC
TaqI 392 TCGA T^CGA
1377 TCGA
Tsp45I 136 GTCAC ^GT[GC]AC
760 GTGAC
TthHB8I 392 TCGA T^CGA
1377 TCGA
XmnI 223 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
349 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI Cac8I
Cfr13I DpnII DsaI EarI Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MnlI MseI MspA1I MspI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI