Restriction map for
pF1KSDA0759
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# NO CUT ENZYMES ( 115 enzymes) #
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AccI AccIII AclI AflII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KspI
MamI MflI MluI MroI NarI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1722 GACGTC GACGT^C
Acc65I 1696 GGTACC G^GTACC
AcyI 1722 GACGTC G[GA]^CG[CT]C
AflIII 76 ACATGT A^C[GA][CT]GT
AhdI 319 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1696 GGTACC G^GTACC
AspEI 319 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 146 CTCGGG C^[CT]CG[GA]G
BbsI 1393 GAAGAC GAAGAC(2/6)
BcgI 987 CGATGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1851 TGATCA T^GATCA
BcnI 1194 CCGGG CC^[GC]GG
BglI 1213 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1393 GAAGAC GAAGAC(2/6)
BsaHI 1722 GACGTC G[GA]^CG[CT]C
BsmI 303 GAATGC GAATGC(1/-1)
BsoBI 146 CTCGGG C^[CT]CG[GA]G
BspHI 645 TCATGA T^CATGA
BspLU11I 76 ACATGT A^CATGT
BsrDI 799 CATTGC GCAATG(2/0)
BsrFI 33 GCCGGC [GA]^CCGG[CT]
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
Eam1105I 319 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 853 GAATTC G^AATTC
EcoRV 874 GATATC GAT^ATC
EcoT22I 1658 ATGCAT ATGCA^T
FbaI 1851 TGATCA T^GATCA
Hin1I 1722 GACGTC G[GA]^CG[CT]C
KpnI 1696 GGTACC GGTAC^C
MfeI 819 CAATTG C^AATTG
MseI 1076 TTAA T^TAA
MunI 819 CAATTG C^AATTG
NaeI 33 GCCGGC GCC^GGC
NciI 1194 CCGGG CC^[GC]GG
NgoMIV 33 GCCGGC G^CCGGC
NsiI 1658 ATGCAT ATGCA^T
NspI 76 ACATGT [GA]CATG^[CT]
RcaI 645 TCATGA T^CATGA
SapI 1955 GCTCTTC GCTCTTC(1/4)
ScaI 1049 AGTACT AGT^ACT
SfiI 1212 GGCCCAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1128 AGGCCT AGG^CCT
Tru9I 1076 TTAA T^TAA
XcmI 1982 CCAGCTTCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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ApaI 150 GGGCCC GGGCC^C
235 GGGCCC
BanI 499 GGTGCC G^G[CT][GA]CC
1696 GGTACC
BseRI 397 GAGGAG GAGGAG(10/8)
966 GAGGAG
BsmBI 1894 CGTCTC CGTCTC(1/5)
1509 GAGACG
BspMI 23 ACCTGC ACCTGC(4/8)
1623 ACCTGC
BstAPI 475 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1268 GCAGCCACTGC
CfoI 42 GCGC GCG^C
53 GCGC
DsaI 137 CCATGG C^C[GA][CT]GG
1359 CCATGG
EcoT14I 137 CCATGG C^C[AT][AT]GG
1359 CCATGG
HgaI 1511 GACGC GACGC(5/10)
7 GCGTC
HhaI 42 GCGC GCG^C
53 GCGC
HinP1I 42 GCGC G^CGC
53 GCGC
MaeII 1539 ACGT A^CGT
1723 ACGT
MslI 735 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1770 CACTACAATG
NcoI 137 CCATGG C^CATGG
1359 CCATGG
NheI 1036 GCTAGC G^CTAGC
1977 GCTAGC
PleI 1639 GAGTC GAGTC(4/5)
1825 GAGTC
StyI 137 CCATGG C^C[AT][AT]GG
1359 CCATGG
TaqI 81 TCGA T^CGA
1866 TCGA
TfiI 1019 GATTC G^A[AT]TC
1315 GATTC
TthHB8I 81 TCGA T^CGA
1866 TCGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaII BalI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TseI
Tsp45I Tsp509I TspRI