Restriction map for
pF1KSDA0733
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BcgI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FseI FspI HapII
HgaI HindIII HpaI HpaII KpnI
KspI MaeII MfeI MluI MroI
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcyI 1724 GGCGCC G[GA]^CG[CT]C
AhdI 768 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1576 GGATC GGATC(4/5)
AlwNI 1777 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1153 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1876 AGCGCT AGC^GCT
AspEI 768 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1994 GAGCTC G[AT]GC[AT]^C
BalI 342 TGGCCA TGG^CCA
BanI 1724 GGCGCC G^G[CT][GA]CC
BanII 1994 GAGCTC G[GA]GC[CT]^C
BbeI 1724 GGCGCC GGCGC^C
BbsI 915 GTCTTC GAAGAC(2/6)
BbvCI 730 CCTCAGC CCTCAGC(-5/-2)
BciVI 82 GTATCC GTATCC(6/5)
BclI 1482 TGATCA T^GATCA
BfaI 494 CTAG C^TAG
BmyI 1994 GAGCTC G[AGT]GC[ACT]^C
BpuAI 915 GTCTTC GAAGAC(2/6)
BsaBI 975 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1724 GGCGCC G[GA]^CG[CT]C
BseRI 1349 CTCCTC GAGGAG(10/8)
BsiHKAI 1994 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1994 GAGCTC G[AGT]GC[ACT]^C
BspMI 44 ACCTGC ACCTGC(4/8)
BstAPI 1243 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1576 GGATCT [GA]^GATC[CT]
Bsu36I 1153 CCTAAGG CC^T[ACGT]AGG
DsaI 267 CCATGG C^C[GA][CT]GG
EaeI 342 TGGCCA [CT]^GGCC[GA]
Eam1105I 768 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1876 AGCGCT AGC^GCT
Eco81I 1153 CCTAAGG CC^T[ACGT]AGG
EcoRV 155 GATATC GAT^ATC
FbaI 1482 TGATCA T^GATCA
Hin1I 1724 GGCGCC G[GA]^CG[CT]C
HincII 1148 GTCAAC GT[CT]^[GA]AC
HindII 1148 GTCAAC GT[CT]^[GA]AC
KasI 1724 GGCGCC G^GCGCC
MaeI 494 CTAG C^TAG
MamI 975 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1576 GGATCT [GA]^GATC[CT]
MluNI 342 TGGCCA TGG^CCA
MscI 342 TGGCCA TGG^CCA
MslI 586 CACATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1780 CAGCTG C[AC]G^C[GT]G
NarI 1724 GGCGCC GG^CGCC
NcoI 267 CCATGG C^CATGG
PaeR7I 1352 CTCGAG C^TCGAG
PflMI 457 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1421 CTGCAG CTGCA^G
PvuII 1780 CAGCTG CAG^CTG
SacI 1994 GAGCTC GAGCT^C
SfcI 1421 CTGCAG C^T[GA][CT]AG
SfoI 1724 GGCGCC GGC^GCC
StuI 646 AGGCCT AGG^CCT
Van91I 457 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1352 CTCGAG C^TCGAG
XhoII 1576 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 209 GTCTAC GT^[AC][GT]AC
847 GTCTAC
AciI 2012 CCGC CCGC(-3/-1)
1203 GCGG
AflIII 1262 ACATGT A^C[GA][CT]GT
1536 ACATGT
AvaI 1352 CTCGAG C^[CT]CG[GA]G
1853 CCCGAG
BsiYI 457 CCACATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1119 CCCAAATACGG
BslI 457 CCACATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1119 CCCAAATACGG
BsmAI 173 GAGAC GTCTC(1/5)
1402 GTCTC
BsmI 1173 GAATGC GAATGC(1/-1)
693 GCATTC
BsoBI 1352 CTCGAG C^[CT]CG[GA]G
1853 CCCGAG
BspLU11I 1262 ACATGT A^CATGT
1536 ACATGT
Cac8I 121 GCCTGC GC[ACGT]^[ACGT]GC
1111 GCCAGC
CfoI 1725 GCGC GCG^C
1877 GCGC
HaeII 1724 GGCGCC [GA]GCGC^[CT]
1876 AGCGCT
HhaI 1725 GCGC GCG^C
1877 GCGC
HinP1I 1725 GCGC G^CGC
1877 GCGC
PleI 303 GACTC GAGTC(4/5)
1968 GACTC
Tsp45I 795 GTCAC ^GT[GC]AC
1363 GTCAC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AvaII BbvI BpmI BsaJI BsmFI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI