Restriction map for
pF1KSDA0669
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BfrI BglII BlnI BsaBI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FspI
HindIII KpnI MamI MfeI MluI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SbfI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AocI 175 CCTGAGG CC^T[ACGT]AGG
AspHI 395 GAGCAC G[AT]GC[AT]^C
BbrPI 1150 CACGTG CAC^GTG
BcgI 1361 CGACTGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1074 GTATCC GTATCC(6/5)
BlpI 1620 GCTGAGC GC^T[ACGT]AGC
BpmI 1364 CTGGAG CTGGAG(16/14)
Bpu1102I 1620 GCTGAGC GC^T[ACGT]AGC
BsaAI 1150 CACGTG [CT]AC^GT[GA]
BsaWI 661 ACCGGA [AT]^CCGG[AT]
BsgI 1396 GTGCAG GTGCAG(16/14)
BsiHKAI 395 GAGCAC G[AT]GC[AT]^C
BsmI 1251 GAATGC GAATGC(1/-1)
BspLU11I 469 ACATGT A^CATGT
BssHII 1268 GCGCGC G^CGCGC
Bsu36I 175 CCTGAGG CC^T[ACGT]AGG
CelII 1620 GCTGAGC GC^T[ACGT]AGC
DraI 2133 TTTAAA TTT^AAA
DraIII 1498 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 531 CGGCCG C^GGCCG
EclXI 531 CGGCCG C^GGCCG
Eco52I 531 CGGCCG C^GGCCG
Eco81I 175 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1307 CCATGG C^C[AT][AT]GG
FseI 1155 GGCCGGCC GGCCGG^CC
HpaI 1887 GTTAAC GTT^AAC
KspI 1046 CCGCGG CCGC^GG
NcoI 1307 CCATGG C^CATGG
PmaCI 1150 CACGTG CAC^GTG
PmlI 1150 CACGTG CAC^GTG
SacII 1046 CCGCGG CCGC^GG
SalI 1771 GTCGAC G^TCGAC
ScaI 616 AGTACT AGT^ACT
SexAI 1485 ACCTGGT A^CC[AT]GGT
SfiI 57 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1194 CACCGGCG C[GA]^CCGG[CT]G
StyI 1307 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 127 GACGTC GACGT^C
148 GACGTC
AlwI 590 GATCC GGATC(4/5)
2082 GGATC
AlwNI 437 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
964 CAGACTCTG
BalI 65 TGGCCA TGG^CCA
1907 TGGCCA
BbsI 1951 GAAGAC GAAGAC(2/6)
1287 GTCTTC
BbvCI 1078 CCTCAGC CCTCAGC(-5/-2)
2333 CCTCAGC
BclI 491 TGATCA T^GATCA
2217 TGATCA
BpuAI 1951 GAAGAC GAAGAC(2/6)
1287 GTCTTC
BsiEI 531 CGGCCG CG[GA][CT]^CG
896 CGACCG
BspMI 1139 ACCTGC ACCTGC(4/8)
1341 GCAGGT
BstF5I 425 CATCC GGATG(2/0)
228 GGATG
BstXI 353 CCAGTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1662 CCAGCATGTTGG
BstYI 589 AGATCC [GA]^GATC[CT]
2082 GGATCT
DraII 951 GGGGCCC [GA]G^G[ACGT]CC[CT]
1026 GGGCCCT
DrdI 571 GACAGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
738 GACTGCTGTGTC
EcoO109I 951 GGGGCCC [GA]G^G[ACGT]CC[CT]
1026 GGGCCCT
FbaI 491 TGATCA T^GATCA
2217 TGATCA
FokI 425 CATCC GGATG(9/13)
228 GGATG
HincII 1771 GTCGAC GT[CT]^[GA]AC
1887 GTTAAC
HindII 1771 GTCGAC GT[CT]^[GA]AC
1887 GTTAAC
MflI 589 AGATCC [GA]^GATC[CT]
2082 GGATCT
MluNI 65 TGGCCA TGG^CCA
1907 TGGCCA
MscI 65 TGGCCA TGG^CCA
1907 TGGCCA
NspI 469 ACATGT [GA]CATG^[CT]
1665 GCATGT
PvuII 277 CAGCTG CAG^CTG
1618 CAGCTG
SapI 24 GAAGAGC GCTCTTC(1/4)
197 GCTCTTC
TaqI 146 TCGA T^CGA
1772 TCGA
Tsp509I 2153 AATT ^AATT
2222 AATT
TthHB8I 146 TCGA T^CGA
1772 TCGA
XhoII 589 AGATCC [GA]^GATC[CT]
2082 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI ApaI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI TfiI
Tru9I TseI Tsp45I TspRI XcmI
XmaCI XmaI