Restriction map for
pF1KSDA0668
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# NO CUT ENZYMES ( 108 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 595 GACGTC GACGT^C
AcsI 877 GAATTT [GA]^AATT[CT]
Aor51HI 1610 AGCGCT AGC^GCT
ApaI 177 GGGCCC GGGCC^C
ApoI 877 GAATTT [GA]^AATT[CT]
Asp700I 630 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2078 GGATCC G^GATCC
BfaI 1003 CTAG C^TAG
BsmBI 474 CGTCTC CGTCTC(1/5)
BsrBI 850 GAGCGG CCGCTC(-3/-3)
BstAPI 1599 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 103 TTTAAA TTT^AAA
DraIII 1588 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1194 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1610 AGCGCT AGC^GCT
FseI 1254 GGCCGGCC GGCCGG^CC
KspI 1520 CCGCGG CCGC^GG
MaeI 1003 CTAG C^TAG
MslI 1479 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1881 GCATGT [GA]CATG^[CT]
PflMI 2061 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 1520 CCGCGG CCGC^GG
SmaI 833 CCCGGG CCC^GGG
StuI 1892 AGGCCT AGG^CCT
TfiI 1360 GAATC G^A[AT]TC
Tsp509I 878 AATT ^AATT
Van91I 2061 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 833 CCCGGG C^CCGGG
XmaI 833 CCCGGG C^CCGGG
XmnI 630 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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Acc65I 563 GGTACC G^GTACC
1745 GGTACC
Alw44I 1582 GTGCAC G^TGCAC
1786 GTGCAC
ApaLI 1582 GTGCAC G^TGCAC
1786 GTGCAC
Asp718I 563 GGTACC G^GTACC
1745 GGTACC
BbvCI 1719 CCTCAGC CCTCAGC(-5/-2)
1230 GCTGAGG
BglI 516 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1724 GCCTCTTCGGC
BlpI 814 GCTGAGC GC^T[ACGT]AGC
1447 GCTCAGC
Bpu1102I 814 GCTGAGC GC^T[ACGT]AGC
1447 GCTCAGC
BsaAI 418 TACGTG [CT]AC^GT[GA]
2055 CACGTA
BsaI 1693 GAGACC GGTCTC(1/5)
1702 GAGACC
BsrFI 521 GCCGGC [GA]^CCGG[CT]
1255 GCCGGC
BssSI 226 CACGAG CACGAG(-5/-1)
973 CACGAG
BstXI 905 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCAGGGAGGTGG
CelII 814 GCTGAGC GC^T[ACGT]AGC
1447 GCTCAGC
Cfr10I 521 GCCGGC [GA]^CCGG[CT]
1255 GCCGGC
DsaI 1520 CCGCGG C^C[GA][CT]GG
1832 CCGTGG
KpnI 563 GGTACC GGTAC^C
1745 GGTACC
MseI 104 TTAA T^TAA
607 TTAA
NaeI 521 GCCGGC GCC^GGC
1255 GCCGGC
NgoMIV 521 GCCGGC G^CCGGC
1255 GCCGGC
PpuMI 121 AGGACCT [GA]G^G[AT]CC[CT]
290 AGGACCT
SapI 386 GCTCTTC GCTCTTC(1/4)
525 GCTCTTC
SexAI 559 ACCTGGT A^CC[AT]GGT
2060 ACCAGGT
Tru9I 104 TTAA T^TAA
607 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XcmI
XhoII