Restriction map for
pF1KSDA0646
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AocI 208 CCTGAGG CC^T[ACGT]AGG
AseI 1369 ATTAAT AT^TAAT
AsnI 1369 ATTAAT AT^TAAT
AspI 753 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 584 TGGCCA TGG^CCA
BamHI 609 GGATCC G^GATCC
BanI 53 GGTGCC G^G[CT][GA]CC
BlpI 910 GCTCAGC GC^T[ACGT]AGC
BpmI 491 CTGGAG CTGGAG(16/14)
Bpu1102I 910 GCTCAGC GC^T[ACGT]AGC
BsaI 29 GAGACC GGTCTC(1/5)
BsgI 899 GTGCAG GTGCAG(16/14)
BsmFI 888 GTCCC GGGAC(10/14)
BspHI 693 TCATGA T^CATGA
BspMI 484 GCAGGT ACCTGC(4/8)
BsrFI 37 GCCGGT [GA]^CCGG[CT]
BstEII 750 GGTGACC G^GT[ACGT]ACC
BstPI 750 GGTGACC G^GT[ACGT]ACC
BstYI 609 GGATCC [GA]^GATC[CT]
Bsu36I 208 CCTGAGG CC^T[ACGT]AGG
CelII 910 GCTCAGC GC^T[ACGT]AGC
Cfr10I 37 GCCGGT [GA]^CCGG[CT]
DpnII 610 GATC ^GATC
Eco81I 208 CCTGAGG CC^T[ACGT]AGG
EcoO65I 750 GGTGACC G^GT[ACGT]ACC
FauI 63 GCGGG CCCGC(4/6)
HaeII 76 AGCGCC [GA]GCGC^[CT]
HgaI 270 GCGTC GACGC(5/10)
HincII 618 GTTGAC GT[CT]^[GA]AC
HindII 618 GTTGAC GT[CT]^[GA]AC
HindIII 732 AAGCTT A^AGCTT
MaeII 1342 ACGT A^CGT
MboI 610 GATC ^GATC
MfeI 1362 CAATTG C^AATTG
MflI 609 GGATCC [GA]^GATC[CT]
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MslI 1174 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1362 CAATTG C^AATTG
NdeII 610 GATC ^GATC
PflFI 753 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 240 GAGTC GAGTC(4/5)
PshBI 1369 ATTAAT AT^TAAT
PstI 1030 CTGCAG CTGCA^G
RcaI 693 TCATGA T^CATGA
SapI 1054 GAAGAGC GCTCTTC(1/4)
Sau3AI 610 GATC ^GATC
SgrAI 36 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 628 CTTGAG C^T[CT][GA]AG
TaqI 180 TCGA T^CGA
Tth111I 753 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 180 TCGA T^CGA
XhoII 609 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 34 CGCG CG^CG
269 CGCG
AcsI 463 AAATTC [GA]^AATT[CT]
510 AAATTT
AfaI 590 GTAC GT^AC
1344 GTAC
AhdI 880 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1327 GACATTAAGTC
AlwI 610 GATCC GGATC(4/5)
609 GGATC
ApoI 463 AAATTC [GA]^AATT[CT]
510 AAATTT
Asp700I 942 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1159 GAAGAAGTTC
AspEI 880 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1327 GACATTAAGTC
AspHI 1198 GAGCTC G[AT]GC[AT]^C
1392 GAGCTC
AvaII 487 GGTCC G^G[AT]CC
1002 GGACC
BbsI 379 GAAGAC GAAGAC(2/6)
942 GAAGAC
BcnI 10 CCCGG CC^[GC]GG
80 CCGGG
BfaI 937 CTAG C^TAG
1189 CTAG
BpuAI 379 GAAGAC GAAGAC(2/6)
942 GAAGAC
BsiHKAI 1198 GAGCTC G[AT]GC[AT]^C
1392 GAGCTC
BsmI 348 GAATGC GAATGC(1/-1)
1306 GAATGC
BstUI 34 CGCG CG^CG
269 CGCG
EaeI 42 TGGCCG [CT]^GGCC[GA]
584 TGGCCA
Eam1105I 880 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1327 GACATTAAGTC
EcoT14I 990 CCAAGG C^C[AT][AT]GG
1124 CCAAGG
MaeI 937 CTAG C^TAG
1189 CTAG
MspA1I 892 CAGCTG C[AC]G^C[GT]G
1015 CAGCTG
MvnI 34 CGCG CG^CG
269 CGCG
NciI 10 CCCGG CC^[GC]GG
80 CCGGG
PpuMI 486 AGGTCCT [GA]G^G[AT]CC[CT]
1001 AGGACCT
PvuII 892 CAGCTG CAG^CTG
1015 CAGCTG
RsaI 590 GTAC GT^AC
1344 GTAC
SacI 1198 GAGCTC GAGCT^C
1392 GAGCTC
SfcI 116 CTGTAG C^T[GA][CT]AG
1030 CTGCAG
StyI 990 CCAAGG C^C[AT][AT]GG
1124 CCAAGG
TspRI 187 CACTG CA[GC]TG[ACGT][ACGT]^
557 CAGTG
XmnI 942 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1159 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I