Restriction map for
pF1KSDA0634
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I BamHI
BclI BfrI BsmI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I CpoI
DraI DraIII DrdI Eco47III EcoT22I
FbaI FseI HincII HindII HpaI
KpnI KspI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfuI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1044 GACGTC GACGT^C
AccI 1599 GTCTAC GT^[AC][GT]AC
AspI 3592 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1496 TGCGCA TGC^GCA
AvrII 904 CCTAGG C^CTAGG
BbrPI 3804 CACGTG CAC^GTG
BbvCI 2997 CCTCAGC CCTCAGC(-5/-2)
BcgI 699 GCAGAAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3363 GGATAC GTATCC(6/5)
BfaI 905 CTAG C^TAG
BglII 3458 AGATCT A^GATCT
BlnI 904 CCTAGG C^CTAGG
BsaBI 603 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 55 CGGCCG CG[GA][CT]^CG
BsiWI 3842 CGTACG C^GTACG
BsmBI 522 CGTCTC CGTCTC(1/5)
BspDI 2974 ATCGAT AT^CGAT
BsrBI 249 GAGCGG CCGCTC(-3/-3)
BssSI 3368 CACGAG CACGAG(-5/-1)
ClaI 2974 ATCGAT AT^CGAT
EagI 55 CGGCCG C^GGCCG
EclXI 55 CGGCCG C^GGCCG
Eco52I 55 CGGCCG C^GGCCG
EcoNI 2496 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3205 GAATTC G^AATTC
EcoRV 2371 GATATC GAT^ATC
FspI 1496 TGCGCA TGC^GCA
MaeI 905 CTAG C^TAG
MamI 603 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3592 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3804 CACGTG CAC^GTG
PmlI 3804 CACGTG CAC^GTG
PshAI 3132 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 723 GAAGAGC GCTCTTC(1/4)
SbfI 3760 CCTGCAGG CCTGCA^GG
ScaI 3085 AGTACT AGT^ACT
SgrAI 9 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3760 CCTGCAGG CCTGCA^GG
Tth111I 3592 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccIII 2346 TCCGGA T^CCGGA
2423 TCCGGA
AcsI 2149 AAATTC [GA]^AATT[CT]
3205 GAATTC
AflIII 3805 ACGTGT A^C[GA][CT]GT
3839 ACACGT
AhdI 1777 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3408 GACGGCCTGTC
Alw44I 475 GTGCAC G^TGCAC
2930 GTGCAC
AocI 1891 CCTGAGG CC^T[ACGT]AGG
3402 CCTGAGG
ApaI 2121 GGGCCC GGGCC^C
3622 GGGCCC
ApaLI 475 GTGCAC G^TGCAC
2930 GTGCAC
ApoI 2149 AAATTC [GA]^AATT[CT]
3205 GAATTC
Asp700I 1585 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2247 GAACAACTTC
AspEI 1777 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3408 GACGGCCTGTC
BalI 1187 TGGCCA TGG^CCA
1685 TGGCCA
BglI 900 GCCACCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2819 GCCGGCCAGGC
BsaAI 3804 CACGTG [CT]AC^GT[GA]
3840 CACGTA
BseAI 2346 TCCGGA T^CCGGA
2423 TCCGGA
Bsp1407I 3194 TGTACA T^GTACA
3470 TGTACA
BspEI 2346 TCCGGA T^CCGGA
2423 TCCGGA
BspHI 747 TCATGA T^CATGA
1452 TCATGA
BsrGI 3194 TGTACA T^GTACA
3470 TGTACA
Bsu36I 1891 CCTGAGG CC^T[ACGT]AGG
3402 CCTGAGG
Eam1105I 1777 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3408 GACGGCCTGTC
EarI 723 GAAGAG CTCTTC(1/4)
988 GAAGAG
Eco81I 1891 CCTGAGG CC^T[ACGT]AGG
3402 CCTGAGG
HindIII 1333 AAGCTT A^AGCTT
1644 AAGCTT
Ksp632I 723 GAAGAG CTCTTC(1/4)
988 GAAGAG
MluNI 1187 TGGCCA TGG^CCA
1685 TGGCCA
MroI 2346 TCCGGA T^CCGGA
2423 TCCGGA
MscI 1187 TGGCCA TGG^CCA
1685 TGGCCA
NspI 1242 GCATGT [GA]CATG^[CT]
2205 GCATGT
PflMI 957 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3121 CCAGCTATTGG
RcaI 747 TCATGA T^CATGA
1452 TCATGA
SacI 2584 GAGCTC GAGCT^C
2680 GAGCTC
SmaI 75 CCCGGG CCC^GGG
312 CCCGGG
SspBI 3194 TGTACA T^GTACA
3470 TGTACA
StuI 1620 AGGCCT AGG^CCT
2918 AGGCCT
Van91I 957 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3121 CCAGCTATTGG
XmaCI 75 CCCGGG C^CCGGG
312 CCCGGG
XmaI 75 CCCGGG C^CCGGG
312 CCCGGG
XmnI 1585 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2247 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII