Restriction map for
pF1KSDA0617
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsgI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuII RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 2195 CGCG CG^CG
AclI 1286 AACGTT AA^CGTT
Alw44I 1736 GTGCAC G^TGCAC
AlwNI 2027 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2272 CCTGAGG CC^T[ACGT]AGG
ApaLI 1736 GTGCAC G^TGCAC
Asp700I 643 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 50 GGATCC G^GATCC
BglII 1671 AGATCT A^GATCT
BsaBI 45 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 163 GAGGAG GAGGAG(10/8)
BsiEI 1056 CGATCG CG[GA][CT]^CG
BsmBI 1090 CGTCTC CGTCTC(1/5)
BsrBI 514 GAGCGG CCGCTC(-3/-3)
BsrDI 1243 GCAATG GCAATG(2/0)
Bst1107I 2287 GTATAC GTA^TAC
BstAPI 1767 GCACATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2195 CGCG CG^CG
BstZ17I 2287 GTATAC GTA^TAC
Bsu36I 2272 CCTGAGG CC^T[ACGT]AGG
Cac8I 556 GCTTGC GC[ACGT]^[ACGT]GC
DraI 1096 TTTAAA TTT^AAA
DraII 101 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 71 CCATGG C^C[GA][CT]GG
EarI 1044 GAAGAG CTCTTC(1/4)
Eco81I 2272 CCTGAGG CC^T[ACGT]AGG
EcoNI 956 CCTATTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 101 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 179 ATGCAT ATGCA^T
HindIII 320 AAGCTT A^AGCTT
Ksp632I 1044 GAAGAG CTCTTC(1/4)
MamI 45 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 776 CAATTG C^AATTG
MspA1I 1921 CAGCGG C[AC]G^C[GT]G
MunI 776 CAATTG C^AATTG
MvnI 2195 CGCG CG^CG
NcoI 71 CCATGG C^CATGG
NsiI 179 ATGCAT ATGCA^T
PpuMI 101 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1286 AACGTT AA^CGTT
PvuI 1056 CGATCG CGAT^CG
SacI 166 GAGCTC GAGCT^C
SalI 1634 GTCGAC G^TCGAC
Tsp45I 1115 GTGAC ^GT[GC]AC
XbaI 2137 TCTAGA T^CTAGA
XmnI 643 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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BanII 166 GAGCTC G[GA]GC[CT]^C
1886 GAGCCC
BbsI 819 GAAGAC GAAGAC(2/6)
880 GAAGAC
BclI 330 TGATCA T^GATCA
1986 TGATCA
BcnI 54 CCGGG CC^[GC]GG
1515 CCGGG
BpmI 1143 CTCCAG CTGGAG(16/14)
1648 CTCCAG
BpuAI 819 GAAGAC GAAGAC(2/6)
880 GAAGAC
BsaI 580 GGTCTC GGTCTC(1/5)
1148 GGTCTC
BsiYI 41 CCAAGATGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
956 CCTATTTGAGG
BslI 41 CCAAGATGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
956 CCTATTTGAGG
BsmFI 101 GGGAC GGGAC(10/14)
1896 GTCCC
CfoI 542 GCGC GCG^C
2194 GCGC
EcoRI 840 GAATTC G^AATTC
1456 GAATTC
EcoRV 1362 GATATC GAT^ATC
1861 GATATC
EcoT14I 71 CCATGG C^C[AT][AT]GG
885 CCTTGG
FauI 1111 GCGGG CCCGC(4/6)
1923 GCGGG
FbaI 330 TGATCA T^GATCA
1986 TGATCA
HaeIII 57 GGCC GG^CC
1538 GGCC
HhaI 542 GCGC GCG^C
2194 GCGC
HinP1I 542 GCGC G^CGC
2194 GCGC
HincII 1015 GTTGAC GT[CT]^[GA]AC
1634 GTCGAC
HindII 1015 GTTGAC GT[CT]^[GA]AC
1634 GTCGAC
MwoI 20 GCACGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1767 GCACATATTGC
NciI 54 CCGGG CC^[GC]GG
1515 CCGGG
NspI 893 GCATGT [GA]CATG^[CT]
1587 ACATGC
PstI 638 CTGCAG CTGCA^G
1667 CTGCAG
SspI 1230 AATATT AAT^ATT
2255 AATATT
StyI 71 CCATGG C^C[AT][AT]GG
885 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbvI BfaI BmyI BsaJI
BsiHKAI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI NdeI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII