Restriction map for
pF1KSDA0585
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaHI BsiWI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII Eam1105I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 544 TCCGGA T^CCGGA
Alw44I 1111 GTGCAC G^TGCAC
AlwNI 579 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 611 AGCGCT AGC^GCT
ApaLI 1111 GTGCAC G^TGCAC
AspI 1177 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 77 GGACC G^G[AT]CC
BalI 742 TGGCCA TGG^CCA
BamHI 1090 GGATCC G^GATCC
BbsI 442 GAAGAC GAAGAC(2/6)
BlpI 108 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 108 GCTGAGC GC^T[ACGT]AGC
BpuAI 442 GAAGAC GAAGAC(2/6)
BsaBI 555 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 912 GAGACC GGTCTC(1/5)
BsaWI 544 TCCGGA [AT]^CCGG[AT]
BseAI 544 TCCGGA T^CCGGA
BsgI 1137 GTGCAG GTGCAG(16/14)
BsmBI 665 GAGACG CGTCTC(1/5)
BspEI 544 TCCGGA T^CCGGA
BsrBI 181 GAGCGG CCGCTC(-3/-3)
BstF5I 24 CATCC GGATG(2/0)
BstYI 1090 GGATCC [GA]^GATC[CT]
CelII 108 GCTGAGC GC^T[ACGT]AGC
DpnII 1091 GATC ^GATC
DraII 505 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1174 GACGACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 611 AGCGCT AGC^GCT
EcoO109I 505 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 751 GAATTC G^AATTC
FokI 24 CATCC GGATG(9/13)
HincII 988 GTTGAC GT[CT]^[GA]AC
HindII 988 GTTGAC GT[CT]^[GA]AC
MaeIII 658 GTGAC ^GT[ACGT]AC
MamI 555 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1091 GATC ^GATC
MflI 1090 GGATCC [GA]^GATC[CT]
MluNI 742 TGGCCA TGG^CCA
MroI 544 TCCGGA T^CCGGA
MscI 742 TGGCCA TGG^CCA
MseI 707 TTAA T^TAA
MslI 651 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 157 CAGCTG C[AC]G^C[GT]G
NdeII 1091 GATC ^GATC
NspI 816 GCATGT [GA]CATG^[CT]
PaeR7I 371 CTCGAG C^TCGAG
PflFI 1177 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 578 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 157 CAGCTG CAG^CTG
SacI 55 GAGCTC GAGCT^C
Sau3AI 1091 GATC ^GATC
SexAI 700 ACCTGGT A^CC[AT]GGT
TfiI 555 GATTC G^A[AT]TC
Tru9I 707 TTAA T^TAA
Tsp45I 658 GTGAC ^GT[GC]AC
Tth111I 1177 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 578 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 371 CTCGAG C^TCGAG
XhoII 1090 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 28 CGCG CG^CG
47 CGCG
AfaI 357 GTAC GT^AC
799 GTAC
AlwI 1091 GATCC GGATC(4/5)
1090 GGATC
AviII 222 TGCGCA TGC^GCA
240 TGCGCA
BanI 453 GGTGCC G^G[CT][GA]CC
617 GGTGCC
BsiEI 49 CGGCCG CG[GA][CT]^CG
119 CGGCCG
BsmI 219 GAATGC GAATGC(1/-1)
588 GAATGC
BstUI 28 CGCG CG^CG
47 CGCG
BstXI 483 CCAGTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCAGGAGGCTGG
Cac8I 503 GCAGGC GC[ACGT]^[ACGT]GC
865 GCCAGC
DsaI 122 CCGTGG C^C[GA][CT]GG
206 CCGTGG
EagI 49 CGGCCG C^GGCCG
119 CGGCCG
EarI 1035 CTCTTC CTCTTC(1/4)
1125 GAAGAG
EclXI 49 CGGCCG C^GGCCG
119 CGGCCG
Eco52I 49 CGGCCG C^GGCCG
119 CGGCCG
FspI 222 TGCGCA TGC^GCA
240 TGCGCA
HaeII 263 AGCGCC [GA]GCGC^[CT]
611 AGCGCT
Ksp632I 1035 CTCTTC CTCTTC(1/4)
1125 GAAGAG
MaeII 134 ACGT A^CGT
1135 ACGT
MvnI 28 CGCG CG^CG
47 CGCG
RsaI 357 GTAC GT^AC
799 GTAC
SfaNI 148 GATGC GCATC(5/9)
23 GCATC
SmlI 58 CTCAAG C^T[CT][GA]AG
371 CTCGAG
XcmI 802 CCAGGAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
875 CCAACTTCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI CfoI Cfr13I
DdeI EaeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI Sau96I ScrFI TaqI TseI
Tsp509I TspRI TthHB8I