Restriction map for
pF1KSDA0547
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI Asp700I
AspEI AspI AviII AvrII BbrPI
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HindIII MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SphI Sse8387I SspBI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1999 GGTACC G^GTACC
AccI 1729 GTAGAC GT^[AC][GT]AC
AccIII 345 TCCGGA T^CCGGA
AflII 2043 CTTAAG C^TTAAG
ApaI 3 GGGCCC GGGCC^C
AseI 1911 ATTAAT AT^TAAT
AsnI 1911 ATTAAT AT^TAAT
Asp718I 1999 GGTACC G^GTACC
BamHI 1568 GGATCC G^GATCC
BbsI 1087 GTCTTC GAAGAC(2/6)
BbvCI 63 CCTCAGC CCTCAGC(-5/-2)
BcgI 696 GCAACATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1934 GTATCC GTATCC(6/5)
BclI 1847 TGATCA T^GATCA
BfrI 2043 CTTAAG C^TTAAG
BglI 1208 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 251 AGATCT A^GATCT
BpuAI 1087 GTCTTC GAAGAC(2/6)
BsaAI 94 TACGTG [CT]AC^GT[GA]
BsaWI 345 TCCGGA [AT]^CCGG[AT]
BseAI 345 TCCGGA T^CCGGA
BsmBI 382 GAGACG CGTCTC(1/5)
BspEI 345 TCCGGA T^CCGGA
BsrBI 148 CCGCTC CCGCTC(-3/-3)
BsrFI 851 GCCGGC [GA]^CCGG[CT]
BstAPI 192 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 851 GCCGGC [GA]^CCGG[CT]
EcoNI 555 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 817 GAATTC G^AATTC
FbaI 1847 TGATCA T^GATCA
HincII 402 GTTAAC GT[CT]^[GA]AC
HindII 402 GTTAAC GT[CT]^[GA]AC
HpaI 402 GTTAAC GTT^AAC
KpnI 1999 GGTACC GGTAC^C
MroI 345 TCCGGA T^CCGGA
MslI 957 CACCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 851 GCCGGC GCC^GGC
NcoI 1903 CCATGG C^CATGG
NdeI 1895 CATATG CA^TATG
NgoMIV 851 GCCGGC G^CCGGC
PaeR7I 580 CTCGAG C^TCGAG
PflMI 495 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1911 ATTAAT AT^TAAT
PstI 323 CTGCAG CTGCA^G
SacI 1869 GAGCTC GAGCT^C
SapI 1948 GAAGAGC GCTCTTC(1/4)
SgrAI 850 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 317 CCCGGG CCC^GGG
SpeI 1930 ACTAGT A^CTAGT
StuI 664 AGGCCT AGG^CCT
Van91I 495 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 545 CCACTCTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 580 CTCGAG C^TCGAG
XmaCI 317 CCCGGG C^CCGGG
XmaI 317 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 817 GAATTC [GA]^AATT[CT]
883 GAATTT
AlwNI 1611 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1957 CAGCTCCTG
ApoI 817 GAATTC [GA]^AATT[CT]
883 GAATTT
AscI 135 GGCGCGCC GG^CGCGCC
223 GGCGCGCC
BbeI 517 GGCGCC GGCGC^C
764 GGCGCC
BsaI 1695 GAGACC GGTCTC(1/5)
1651 GGTCTC
BsmI 1443 GCATTC GAATGC(1/-1)
1602 GCATTC
DraIII 193 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1425 CACTTTGTG
EarI 1065 GAAGAG CTCTTC(1/4)
1948 GAAGAG
HphI 156 TCACC GGTGA(8/7)
541 TCACC
KasI 517 GGCGCC G^GCGCC
764 GGCGCC
Ksp632I 1065 GAAGAG CTCTTC(1/4)
1948 GAAGAG
NarI 517 GGCGCC GG^CGCC
764 GGCGCC
SfaNI 467 GCATC GCATC(5/9)
897 GCATC
SfcI 323 CTGCAG C^T[GA][CT]AG
1859 CTACAG
SfoI 517 GGCGCC GGC^GCC
764 GGCGCC
SspI 865 AATATT AAT^ATT
1478 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII