Restriction map for
pF1KSDA0475
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 809 CGCG CG^CG
AcyI 1117 GACGCC G[GA]^CG[CT]C
Alw44I 1164 GTGCAC G^TGCAC
ApaLI 1164 GTGCAC G^TGCAC
AvaI 218 CCCGGG C^[CT]CG[GA]G
BanI 156 GGCACC G^G[CT][GA]CC
BanII 482 GAGCCC G[GA]GC[CT]^C
BbsI 1201 GAAGAC GAAGAC(2/6)
BfaI 905 CTAG C^TAG
BpuAI 1201 GAAGAC GAAGAC(2/6)
BsaBI 1105 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1117 GACGCC G[GA]^CG[CT]C
BsaI 386 GAGACC GGTCTC(1/5)
BsmI 968 GCATTC GAATGC(1/-1)
BsoBI 218 CCCGGG C^[CT]CG[GA]G
BsrDI 857 GCAATG GCAATG(2/0)
BstUI 809 CGCG CG^CG
BstXI 676 CCAGATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 655 GGATCT [GA]^GATC[CT]
DraII 115 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 829 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 235 GAAGAG CTCTTC(1/4)
EcoO109I 115 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 262 ATGCAT ATGCA^T
HaeII 901 GGCGCT [GA]GCGC^[CT]
HgaI 1117 GACGC GACGC(5/10)
Hin1I 1117 GACGCC G[GA]^CG[CT]C
HindIII 936 AAGCTT A^AGCTT
Ksp632I 235 GAAGAG CTCTTC(1/4)
MaeI 905 CTAG C^TAG
MaeII 787 ACGT A^CGT
MamI 1105 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 655 GGATCT [GA]^GATC[CT]
MvnI 809 CGCG CG^CG
NsiI 262 ATGCAT ATGCA^T
NspI 264 GCATGC [GA]CATG^[CT]
PstI 691 CTGCAG CTGCA^G
ScaI 1191 AGTACT AGT^ACT
SmaI 218 CCCGGG CCC^GGG
SphI 264 GCATGC GCATG^C
TfiI 465 GATTC G^A[AT]TC
XhoII 655 GGATCT [GA]^GATC[CT]
XmaCI 218 CCCGGG C^CCGGG
XmaI 218 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 228 GTAC GT^AC
1192 GTAC
AlwI 1105 GATCC GGATC(4/5)
655 GGATC
BsmFI 313 GGGAC GGGAC(10/14)
789 GTCCC
CfoI 810 GCGC GCG^C
902 GCGC
FauI 807 CCCGC CCCGC(4/6)
144 GCGGG
HapII 102 CCGG C^CGG
219 CCGG
HhaI 810 GCGC GCG^C
902 GCGC
HinP1I 810 GCGC G^CGC
902 GCGC
HpaII 102 CCGG C^CGG
219 CCGG
MaeIII 661 GTCAC ^GT[ACGT]AC
704 GTCAC
MslI 871 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
999 CATTCGGGTG
MspI 102 CCGG C^CGG
219 CCGG
PflMI 204 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCATGTGGTGG
PleI 799 GACTC GAGTC(4/5)
17 GAGTC
RsaI 228 GTAC GT^AC
1192 GTAC
SfcI 569 CTGTAG C^T[GA][CT]AG
691 CTGCAG
Tsp45I 661 GTCAC ^GT[GC]AC
704 GTCAC
Tsp509I 33 AATT ^AATT
737 AATT
Van91I 204 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCATGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MboI MboII MluNI
MnlI MscI MseI MspA1I MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI TspRI