Restriction map for
pF1KSDA0457
##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BcgI BciVI BclI BfaI BlnI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
FspI KpnI KspI MaeI MfeI
MluI MluNI MscI MunI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 284 TCCGGA T^CCGGA
AcsI 1705 AAATTC [GA]^AATT[CT]
AflIII 1346 ACGTGT A^C[GA][CT]GT
AlwNI 149 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1705 AAATTC [GA]^AATT[CT]
AvaI 267 CTCGAG C^[CT]CG[GA]G
BamHI 915 GGATCC G^GATCC
BbeI 22 GGCGCC GGCGC^C
BbrPI 1345 CACGTG CAC^GTG
BglI 384 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2349 AGATCT A^GATCT
BlpI 393 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 393 GCTTAGC GC^T[ACGT]AGC
BsaAI 1345 CACGTG [CT]AC^GT[GA]
BsaBI 2344 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 284 TCCGGA T^CCGGA
BsmI 873 GCATTC GAATGC(1/-1)
BsoBI 267 CTCGAG C^[CT]CG[GA]G
BspEI 284 TCCGGA T^CCGGA
BspHI 2340 TCATGA T^CATGA
BspMI 87 ACCTGC ACCTGC(4/8)
BsrDI 712 CATTGC GCAATG(2/0)
BstAPI 551 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1216 GGTCACC G^GT[ACGT]ACC
BstPI 1216 GGTCACC G^GT[ACGT]ACC
BstXI 905 CCAGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 393 GCTTAGC GC^T[ACGT]AGC
EaeI 400 TGGCCG [CT]^GGCC[GA]
EarI 1949 GAAGAG CTCTTC(1/4)
EcoNI 1818 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1216 GGTCACC G^GT[ACGT]ACC
EcoT14I 446 CCATGG C^C[AT][AT]GG
HaeII 22 GGCGCC [GA]GCGC^[CT]
HgaI 469 GACGC GACGC(5/10)
HincII 1732 GTTAAC GT[CT]^[GA]AC
HindII 1732 GTTAAC GT[CT]^[GA]AC
HpaI 1732 GTTAAC GTT^AAC
KasI 22 GGCGCC G^GCGCC
Ksp632I 1949 GAAGAG CTCTTC(1/4)
MamI 2344 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 284 TCCGGA T^CCGGA
MslI 2335 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 37 GCCGGC GCC^GGC
NarI 22 GGCGCC GG^CGCC
NcoI 446 CCATGG C^CATGG
NgoMIV 37 GCCGGC G^CCGGC
PaeR7I 267 CTCGAG C^TCGAG
PmaCI 1345 CACGTG CAC^GTG
PmlI 1345 CACGTG CAC^GTG
RcaI 2340 TCATGA T^CATGA
SacI 2374 GAGCTC GAGCT^C
SfoI 22 GGCGCC GGC^GCC
SgrAI 36 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1779 GCATGC GCATG^C
StyI 446 CCATGG C^C[AT][AT]GG
Tsp509I 1706 AATT ^AATT
XhoI 267 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 61 CGCG CG^CG
776 CGCG
AcyI 22 GGCGCC G[GA]^CG[CT]C
469 GACGCC
AfaI 2010 GTAC GT^AC
2304 GTAC
AflII 1056 CTTAAG C^TTAAG
2023 CTTAAG
AspHI 1960 GAGCAC G[AT]GC[AT]^C
2374 GAGCTC
BanI 22 GGCGCC G^G[CT][GA]CC
373 GGCACC
BbvCI 1583 CCTCAGC CCTCAGC(-5/-2)
1872 CCTCAGC
BfrI 1056 CTTAAG C^TTAAG
2023 CTTAAG
BsaHI 22 GGCGCC G[GA]^CG[CT]C
469 GACGCC
BsaI 460 GAGACC GGTCTC(1/5)
816 GGTCTC
BsiHKAI 1960 GAGCAC G[AT]GC[AT]^C
2374 GAGCTC
BsmBI 1120 GAGACG CGTCTC(1/5)
1359 GAGACG
BsrFI 37 GCCGGC [GA]^CCGG[CT]
1588 GCCGGT
BssSI 766 CACGAG CACGAG(-5/-1)
1356 CACGAG
BstUI 61 CGCG CG^CG
776 CGCG
BstYI 915 GGATCC [GA]^GATC[CT]
2349 AGATCT
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
1588 GCCGGT
DsaI 446 CCATGG C^C[GA][CT]GG
1251 CCGTGG
EcoT22I 871 ATGCAT ATGCA^T
1777 ATGCAT
Hin1I 22 GGCGCC G[GA]^CG[CT]C
469 GACGCC
HindIII 624 AAGCTT A^AGCTT
2087 AAGCTT
MflI 915 GGATCC [GA]^GATC[CT]
2349 AGATCT
MvnI 61 CGCG CG^CG
776 CGCG
NsiI 871 ATGCAT ATGCA^T
1777 ATGCAT
NspI 869 ACATGC [GA]CATG^[CT]
1779 GCATGC
PflMI 726 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2053 CCACTGCTTGG
RsaI 2010 GTAC GT^AC
2304 GTAC
SfaNI 964 GATGC GCATC(5/9)
1087 GATGC
StuI 273 AGGCCT AGG^CCT
1562 AGGCCT
Van91I 726 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2053 CCACTGCTTGG
XhoII 915 GGATCC [GA]^GATC[CT]
2349 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwI AocI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I