Restriction map for
pF1KE2501
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpmI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 103 GTCTAC GT^[AC][GT]AC
AccII 538 CGCG CG^CG
AflIII 62 ACGTGT A^C[GA][CT]GT
AocI 670 CCTAAGG CC^T[ACGT]AGG
ApaI 709 GGGCCC GGGCC^C
Asp700I 51 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 283 GAGCTC G[AT]GC[AT]^C
AvaII 167 GGACC G^G[AT]CC
BlpI 350 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 350 GCTCAGC GC^T[ACGT]AGC
BsaAI 61 TACGTG [CT]AC^GT[GA]
BsgI 772 GTGCAG GTGCAG(16/14)
BsiHKAI 283 GAGCTC G[AT]GC[AT]^C
BspHI 845 TCATGA T^CATGA
BspMI 774 GCAGGT ACCTGC(4/8)
BsrDI 76 GCAATG GCAATG(2/0)
BstUI 538 CGCG CG^CG
BstXI 743 CCAATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 670 CCTAAGG CC^T[ACGT]AGG
CelII 350 GCTCAGC GC^T[ACGT]AGC
EarI 150 GAAGAG CTCTTC(1/4)
Eco81I 670 CCTAAGG CC^T[ACGT]AGG
EcoNI 843 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 453 GCGGG CCCGC(4/6)
Ksp632I 150 GAAGAG CTCTTC(1/4)
MaeII 62 ACGT A^CGT
MspA1I 814 CAGCTG C[AC]G^C[GT]G
MvnI 538 CGCG CG^CG
PaeR7I 954 CTCGAG C^TCGAG
PleI 952 GACTC GAGTC(4/5)
PpuMI 166 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 814 CAGCTG CAG^CTG
RcaI 845 TCATGA T^CATGA
SacI 283 GAGCTC GAGCT^C
SapI 150 GAAGAGC GCTCTTC(1/4)
SbfI 519 CCTGCAGG CCTGCA^GG
Sse8387I 519 CCTGCAGG CCTGCA^GG
Tsp45I 271 GTCAC ^GT[GC]AC
XcmI 890 CCATTGTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 954 CTCGAG C^TCGAG
XmnI 51 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 705 CTCGGG C^[CT]CG[GA]G
954 CTCGAG
BalI 258 TGGCCA TGG^CCA
489 TGGCCA
BanII 283 GAGCTC G[GA]GC[CT]^C
709 GGGCCC
BcnI 128 CCCGG CC^[GC]GG
290 CCCGG
BsmFI 166 GGGAC GGGAC(10/14)
468 GGGAC
BsoBI 705 CTCGGG C^[CT]CG[GA]G
954 CTCGAG
BstAPI 513 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
721 GCAGCCACTGC
BstYI 188 AGATCC [GA]^GATC[CT]
515 AGATCC
Cac8I 195 GCGAGC GC[ACGT]^[ACGT]GC
522 GCAGGC
DsaI 162 CCATGG C^C[GA][CT]GG
749 CCATGG
HapII 129 CCGG C^CGG
291 CCGG
HpaII 129 CCGG C^CGG
291 CCGG
MaeIII 271 GTCAC ^GT[ACGT]AC
320 GTAAC
MflI 188 AGATCC [GA]^GATC[CT]
515 AGATCC
MluNI 258 TGGCCA TGG^CCA
489 TGGCCA
MscI 258 TGGCCA TGG^CCA
489 TGGCCA
MspI 129 CCGG C^CGG
291 CCGG
NciI 128 CCCGG CC^[GC]GG
290 CCCGG
NcoI 162 CCATGG C^CATGG
749 CCATGG
PstI 520 CTGCAG CTGCA^G
719 CTGCAG
SmlI 919 CTCAAG C^T[CT][GA]AG
954 CTCGAG
TfiI 642 GATTC G^A[AT]TC
866 GAATC
XhoII 188 AGATCC [GA]^GATC[CT]
515 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I