Restriction map for
pF1KSDA0362
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflII AflIII Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BclI BfaI
BfrI BlnI BsaBI BsiWI Bsp1407I
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CpoI DraI
DraIII DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MaeI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RsrII SexAI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AgeI 1522 ACCGGT A^CCGGT
Aor51HI 242 AGCGCT AGC^GCT
AspI 196 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2787 GGCGCC GGCGC^C
BbsI 1615 GTCTTC GAAGAC(2/6)
BciVI 3004 GGATAC GTATCC(6/5)
BglI 1850 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1615 GTCTTC GAAGAC(2/6)
BsaI 1519 GAGACC GGTCTC(1/5)
BsmBI 1402 GAGACG CGTCTC(1/5)
BspDI 628 ATCGAT AT^CGAT
BspHI 350 TCATGA T^CATGA
BstXI 3164 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 628 ATCGAT AT^CGAT
EagI 170 CGGCCG C^GGCCG
EclXI 170 CGGCCG C^GGCCG
Eco47III 242 AGCGCT AGC^GCT
Eco52I 170 CGGCCG C^GGCCG
EcoT14I 2549 CCAAGG C^C[AT][AT]GG
KasI 2787 GGCGCC G^GCGCC
NarI 2787 GGCGCC GG^CGCC
PflFI 196 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1522 ACCGGT A^CCGGT
RcaI 350 TCATGA T^CATGA
SalI 804 GTCGAC G^TCGAC
SapI 2616 GCTCTTC GCTCTTC(1/4)
ScaI 2186 AGTACT AGT^ACT
SfoI 2787 GGCGCC GGC^GCC
SmaI 1232 CCCGGG CCC^GGG
SphI 101 GCATGC GCATG^C
StuI 2025 AGGCCT AGG^CCT
StyI 2549 CCAAGG C^C[AT][AT]GG
Tth111I 196 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1232 CCCGGG C^CCGGG
XmaI 1232 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AatII 796 GACGTC GACGT^C
1404 GACGTC
AccIII 327 TCCGGA T^CCGGA
1805 TCCGGA
AcsI 553 AAATTC [GA]^AATT[CT]
2825 AAATTC
AhdI 796 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACGCAGCGTC
AlwI 1539 GATCC GGATC(4/5)
2318 GGATC
ApoI 553 AAATTC [GA]^AATT[CT]
2825 AAATTC
AspEI 796 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACGCAGCGTC
AvaI 1232 CCCGGG C^[CT]CG[GA]G
1723 CCCGAG
BbrPI 1603 CACGTG CAC^GTG
1909 CACGTG
BcgI 1791 CGAGGGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
701 GCACGGCCATCG
BseAI 327 TCCGGA T^CCGGA
1805 TCCGGA
BsiEI 170 CGGCCG CG[GA][CT]^CG
3191 CGGTCG
BsmI 2254 GAATGC GAATGC(1/-1)
1760 GCATTC
BsoBI 1232 CCCGGG C^[CT]CG[GA]G
1723 CCCGAG
BspEI 327 TCCGGA T^CCGGA
1805 TCCGGA
BssHII 122 GCGCGC G^CGCGC
2761 GCGCGC
BstAPI 1053 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2363 GCAGGAACTGC
Eam1105I 796 GACGTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1111 GACGCAGCGTC
EarI 2617 CTCTTC CTCTTC(1/4)
2628 GAAGAG
EcoNI 1817 CCTACCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2388 CCTGCAGGAGG
HincII 804 GTCGAC GT[CT]^[GA]AC
2019 GTCAAC
HindII 804 GTCGAC GT[CT]^[GA]AC
2019 GTCAAC
Ksp632I 2617 CTCTTC CTCTTC(1/4)
2628 GAAGAG
KspI 1860 CCGCGG CCGC^GG
1982 CCGCGG
MroI 327 TCCGGA T^CCGGA
1805 TCCGGA
NaeI 1850 GCCGGC GCC^GGC
2913 GCCGGC
NgoMIV 1850 GCCGGC G^CCGGC
2913 GCCGGC
NspI 101 GCATGC [GA]CATG^[CT]
1164 GCATGT
PflMI 1409 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACTCACTGG
PmaCI 1603 CACGTG CAC^GTG
1909 CACGTG
PmlI 1603 CACGTG CAC^GTG
1909 CACGTG
SacII 1860 CCGCGG CCGC^GG
1982 CCGCGG
SbfI 378 CCTGCAGG CCTGCA^GG
2388 CCTGCAGG
Sse8387I 378 CCTGCAGG CCTGCA^GG
2388 CCTGCAGG
Tsp45I 1328 GTGAC ^GT[GC]AC
2895 GTCAC
Tsp509I 554 AATT ^AATT
2826 AATT
Van91I 1409 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI AocI ApaI AspHI
AvaII AviII BalI BanI BanII
BbvCI BbvI BcnI BglII BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI TspRI
TthHB8I XhoII