Restriction map for
pF1KSDA0331
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BsaAI BsgI
BsiEI BsiWI BspDI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CfoI Cfr10I ClaI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 1345 GACGTC GACGT^C
AccII 9 CGCG CG^CG
AccIII 1673 TCCGGA T^CCGGA
AcyI 1345 GACGTC G[GA]^CG[CT]C
AhdI 60 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2199 GTGCAC G^TGCAC
AlwNI 1940 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2199 GTGCAC G^TGCAC
AspEI 60 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1595 CTCGAG C^[CT]CG[GA]G
BalI 1335 TGGCCA TGG^CCA
BanII 115 GAGCTC G[GA]GC[CT]^C
BglII 484 AGATCT A^GATCT
BlpI 1705 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1705 GCTCAGC GC^T[ACGT]AGC
BsaBI 190 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1345 GACGTC G[GA]^CG[CT]C
BsaI 1598 GAGACC GGTCTC(1/5)
BsaWI 1673 TCCGGA [AT]^CCGG[AT]
BseAI 1673 TCCGGA T^CCGGA
BsmBI 593 GAGACG CGTCTC(1/5)
BsoBI 1595 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1842 TGTACA T^GTACA
BspEI 1673 TCCGGA T^CCGGA
BspMI 1248 ACCTGC ACCTGC(4/8)
BsrGI 1842 TGTACA T^GTACA
BstAPI 1622 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 9 CGCG CG^CG
CelII 1705 GCTCAGC GC^T[ACGT]AGC
CpoI 1991 CGGTCCG CG^G[AT]CCG
Eam1105I 60 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2134 GAATTC G^AATTC
EcoRV 443 GATATC GAT^ATC
EcoT22I 312 ATGCAT ATGCA^T
Hin1I 1345 GACGTC G[GA]^CG[CT]C
HindIII 2221 AAGCTT A^AGCTT
KspI 8 CCGCGG CCGC^GG
MamI 190 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1335 TGGCCA TGG^CCA
MroI 1673 TCCGGA T^CCGGA
MscI 1335 TGGCCA TGG^CCA
MvnI 9 CGCG CG^CG
NcoI 2122 CCATGG C^CATGG
NdeI 1067 CATATG CA^TATG
NsiI 312 ATGCAT ATGCA^T
PaeR7I 1595 CTCGAG C^TCGAG
RsrII 1991 CGGTCCG CG^G[AT]CCG
SacI 115 GAGCTC GAGCT^C
SacII 8 CCGCGG CCGC^GG
SapI 207 GCTCTTC GCTCTTC(1/4)
ScaI 1789 AGTACT AGT^ACT
SfcI 573 CTACAG C^T[GA][CT]AG
SphI 1014 GCATGC GCATG^C
SspBI 1842 TGTACA T^GTACA
StuI 1132 AGGCCT AGG^CCT
XbaI 1434 TCTAGA T^CTAGA
XcmI 2306 CCAGGCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1595 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 676 AAATTT [GA]^AATT[CT]
2134 GAATTC
AflIII 1034 ACATGT A^C[GA][CT]GT
1559 ACATGT
AocI 1923 CCTAAGG CC^T[ACGT]AGG
2260 CCTCAGG
ApoI 676 AAATTT [GA]^AATT[CT]
2134 GAATTC
Asp700I 1426 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1438 GAAGAACTTC
BamHI 456 GGATCC G^GATCC
1458 GGATCC
BbsI 709 GAAGAC GAAGAC(2/6)
1878 GAAGAC
BbvCI 240 CCTCAGC CCTCAGC(-5/-2)
1327 GCTGAGG
BcnI 92 CCCGG CC^[GC]GG
1634 CCCGG
BfaI 1040 CTAG C^TAG
1435 CTAG
BpuAI 709 GAAGAC GAAGAC(2/6)
1878 GAAGAC
BsmI 310 GAATGC GAATGC(1/-1)
1043 GCATTC
BspLU11I 1034 ACATGT A^CATGT
1559 ACATGT
Bsu36I 1923 CCTAAGG CC^T[ACGT]AGG
2260 CCTCAGG
DsaI 8 CCGCGG C^C[GA][CT]GG
2122 CCATGG
EaeI 283 TGGCCG [CT]^GGCC[GA]
1335 TGGCCA
Eco81I 1923 CCTAAGG CC^T[ACGT]AGG
2260 CCTCAGG
FauI 10 GCGGG CCCGC(4/6)
334 GCGGG
HgaI 595 GACGC GACGC(5/10)
657 GCGTC
MaeI 1040 CTAG C^TAG
1435 CTAG
MwoI 754 GCACTGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1622 GCATATCCTGC
NciI 92 CCCGG CC^[GC]GG
1634 CCCGG
SmlI 1535 CTCAAG C^T[CT][GA]AG
1595 CTCGAG
XmnI 1426 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1438 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII