Restriction map for
pF1KSDA0083
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# NO CUT ENZYMES ( 110 enzymes) #
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AccII AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FauI FbaI FseI FspI
HgaI KasI KspI MamI MflI
MluI MroI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 2993 GACGTC GACGT^C
Acc65I 137 GGTACC G^GTACC
AccI 2896 GTAGAC GT^[AC][GT]AC
AcyI 2993 GACGTC G[GA]^CG[CT]C
AflII 2505 CTTAAG C^TTAAG
AgeI 134 ACCGGT A^CCGGT
AocI 243 CCTTAGG CC^T[ACGT]AGG
ApaI 2319 GGGCCC GGGCC^C
AseI 3111 ATTAAT AT^TAAT
AsnI 3111 ATTAAT AT^TAAT
Asp700I 1727 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 137 GGTACC G^GTACC
AvrII 802 CCTAGG C^CTAGG
BalI 2558 TGGCCA TGG^CCA
BbsI 1128 GAAGAC GAAGAC(2/6)
BfrI 2505 CTTAAG C^TTAAG
BglI 1884 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 802 CCTAGG C^CTAGG
BpuAI 1128 GAAGAC GAAGAC(2/6)
BsaHI 2993 GACGTC G[GA]^CG[CT]C
BsaWI 134 ACCGGT [AT]^CCGG[AT]
BseRI 49 GAGGAG GAGGAG(10/8)
BsmAI 302 GAGAC GTCTC(1/5)
BspMI 1540 GCAGGT ACCTGC(4/8)
BsrDI 2735 GCAATG GCAATG(2/0)
BstXI 1736 CCAAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 243 CCTTAGG CC^T[ACGT]AGG
DraII 2319 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 2558 TGGCCA [CT]^GGCC[GA]
Eco81I 243 CCTTAGG CC^T[ACGT]AGG
EcoNI 1948 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2319 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1988 GAATTC G^AATTC
EcoRV 283 GATATC GAT^ATC
Hin1I 2993 GACGTC G[GA]^CG[CT]C
HindIII 2421 AAGCTT A^AGCTT
KpnI 137 GGTACC GGTAC^C
MluNI 2558 TGGCCA TGG^CCA
MscI 2558 TGGCCA TGG^CCA
MslI 120 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 63 GCCGGC GCC^GGC
NgoMIV 63 GCCGGC G^CCGGC
PinAI 134 ACCGGT A^CCGGT
PleI 1351 GAGTC GAGTC(4/5)
PshBI 3111 ATTAAT AT^TAAT
PvuII 7 CAGCTG CAG^CTG
SexAI 140 ACCTGGT A^CC[AT]GGT
XmnI 1727 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 983 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1291 CAGCATCTG
BciVI 1848 GGATAC GTATCC(6/5)
357 GTATCC
BsgI 306 CTGCAC GTGCAG(16/14)
2473 GTGCAG
BsmI 1084 GCATTC GAATGC(1/-1)
1862 GCATTC
BsrFI 63 GCCGGC [GA]^CCGG[CT]
134 ACCGGT
CfoI 192 GCGC GCG^C
2704 GCGC
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
134 ACCGGT
EcoT22I 237 ATGCAT ATGCA^T
673 ATGCAT
HaeII 191 AGCGCC [GA]GCGC^[CT]
2703 AGCGCC
HaeIII 2320 GGCC GG^CC
2559 GGCC
HapII 64 CCGG C^CGG
135 CCGG
HhaI 192 GCGC GCG^C
2704 GCGC
HinP1I 192 GCGC G^CGC
2704 GCGC
HpaI 1341 GTTAAC GTT^AAC
2466 GTTAAC
HpaII 64 CCGG C^CGG
135 CCGG
MfeI 1200 CAATTG C^AATTG
1256 CAATTG
MspA1I 7 CAGCTG C[AC]G^C[GT]G
886 CCGCTG
MspI 64 CCGG C^CGG
135 CCGG
MunI 1200 CAATTG C^AATTG
1256 CAATTG
NsiI 237 ATGCAT ATGCA^T
673 ATGCAT
PflMI 982 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3153 CCACATACTGG
SacI 441 GAGCTC GAGCT^C
2402 GAGCTC
SmlI 1006 CTCAAG C^T[CT][GA]AG
2505 CTTAAG
Van91I 982 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3153 CCACATACTGG
XcmI 462 CCAAATGCTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1500 CCAATGTAAACATGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI