Restriction map for
pF1KB8009
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# NO CUT ENZYMES ( 146 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FauI
FseI FspI HaeII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
HphI KasI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NarI NciI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 75 GACGTC GACGT^C
Acc65I 879 GGTACC G^GTACC
AciI 435 GCGG CCGC(-3/-1)
AcyI 75 GACGTC G[GA]^CG[CT]C
AluI 983 AGCT AG^CT
Alw44I 452 GTGCAC G^TGCAC
ApaLI 452 GTGCAC G^TGCAC
Asp700I 700 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 879 GGTACC G^GTACC
AvaII 121 GGTCC G^G[AT]CC
BanI 879 GGTACC G^G[CT][GA]CC
BanII 938 GAGCCC G[GA]GC[CT]^C
BbvI 550 GCTGC GCAGC(8/12)
BciVI 996 GGATAC GTATCC(6/5)
BclI 819 TGATCA T^GATCA
BsaAI 61 TACGTG [CT]AC^GT[GA]
BsaHI 75 GACGTC G[GA]^CG[CT]C
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiYI 779 CCCAATACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 779 CCCAATACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 73 GAGACG CGTCTC(1/5)
BspMI 882 ACCTGC ACCTGC(4/8)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BssSI 935 CACGAG CACGAG(-5/-1)
BstXI 1128 CCAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DsaI 579 CCATGG C^C[GA][CT]GG
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoRV 145 GATATC GAT^ATC
EcoT14I 579 CCATGG C^C[AT][AT]GG
FbaI 819 TGATCA T^GATCA
Hin1I 75 GACGTC G[GA]^CG[CT]C
KpnI 879 GGTACC GGTAC^C
MseI 1052 TTAA T^TAA
MslI 790 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 982 CAGCTG C[AC]G^C[GT]G
NaeI 39 GCCGGC GCC^GGC
NcoI 579 CCATGG C^CATGG
NgoMIV 39 GCCGGC G^CCGGC
NspI 525 GCATGC [GA]CATG^[CT]
PstI 902 CTGCAG CTGCA^G
PvuII 982 CAGCTG CAG^CTG
SapI 1023 GCTCTTC GCTCTTC(1/4)
SfaNI 789 GCATC GCATC(5/9)
SmlI 691 CTTGAG C^T[CT][GA]AG
SphI 525 GCATGC GCATG^C
SspI 644 AATATT AAT^ATT
StyI 579 CCATGG C^C[AT][AT]GG
Tru9I 1052 TTAA T^TAA
TseI 550 GCTGC G^C[AT]GC
Tsp45I 835 GTCAC ^GT[GC]AC
XmnI 700 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspHI 452 GTGCAC G[AT]GC[AT]^C
797 GTGCTC
BbsI 460 GAAGAC GAAGAC(2/6)
152 GTCTTC
BpmI 188 CTGGAG CTGGAG(16/14)
678 CTGGAG
BpuAI 460 GAAGAC GAAGAC(2/6)
152 GTCTTC
BsgI 281 CTGCAC GTGCAG(16/14)
49 GTGCAG
BsiHKAI 452 GTGCAC G[AT]GC[AT]^C
797 GTGCTC
BsmI 503 GAATGC GAATGC(1/-1)
1010 GAATGC
BspHI 246 TCATGA T^CATGA
1028 TCATGA
Cfr13I 121 GGTCC G^G[ACGT]CC
777 GGCCC
DdeI 220 CTTAG C^T[ACGT]AG
338 CTCAG
EaeI 41 CGGCCG [CT]^GGCC[GA]
436 CGGCCA
Fnu4HI 435 GCGGC GC^[ACGT]GC
550 GCTGC
HapII 40 CCGG C^CGG
114 CCGG
HpaII 40 CCGG C^CGG
114 CCGG
ItaI 435 GCGGC GC^[ACGT]GC
550 GCTGC
MaeII 62 ACGT A^CGT
76 ACGT
MaeIII 349 GTAAC ^GT[ACGT]AC
835 GTCAC
MspI 40 CCGG C^CGG
114 CCGG
MwoI 1014 GCTAAAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1107 GCAGAGACAGC
PleI 382 GACTC GAGTC(4/5)
730 GAGTC
RcaI 246 TCATGA T^CATGA
1028 TCATGA
Sau96I 121 GGTCC G^G[ACGT]CC
777 GGCCC
SfcI 465 CTACAG C^T[GA][CT]AG
902 CTGCAG
TspRI 676 CACTG CA[GC]TG[ACGT][ACGT]^
1117 CAGTG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI ApoI BfaI BmyI
BsaJI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DpnII
EarI EcoRII FokI HaeIII HinfI
Ksp632I MaeI MboI MboII MnlI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI TfiI Tsp509I