Restriction map for
pF1KB6002
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccII AcyI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BsaHI BsiEI BsiWI
BsoBI BsrBI BsrFI BssHII BssSI
BstAPI BstUI Cfr10I CpoI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoRI FseI FspI
Hin1I HpaI KasI KpnI KspI
MluI MvnI NaeI NarI NcoI
NgoMIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 4882 GTATAC GT^[AC][GT]AC
AccIII 51 TCCGGA T^CCGGA
AclI 2693 AACGTT AA^CGTT
AspHI 3095 GTGCTC G[AT]GC[AT]^C
BalI 424 TGGCCA TGG^CCA
BcgI 1773 CGAGACTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2853 TGATCA T^GATCA
BcnI 568 CCGGG CC^[GC]GG
BlpI 4180 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4180 GCTCAGC GC^T[ACGT]AGC
BsaAI 1317 TACGTG [CT]AC^GT[GA]
BsaI 1275 GGTCTC GGTCTC(1/5)
BsaWI 51 TCCGGA [AT]^CCGG[AT]
BseAI 51 TCCGGA T^CCGGA
BsgI 4943 GTGCAG GTGCAG(16/14)
BsiHKAI 3095 GTGCTC G[AT]GC[AT]^C
BspDI 268 ATCGAT AT^CGAT
BspEI 51 TCCGGA T^CCGGA
BspHI 1467 TCATGA T^CATGA
Bst1107I 4882 GTATAC GTA^TAC
BstEII 4022 GGTTACC G^GT[ACGT]ACC
BstPI 4022 GGTTACC G^GT[ACGT]ACC
BstXI 2993 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4882 GTATAC GTA^TAC
CelII 4180 GCTCAGC GC^T[ACGT]AGC
ClaI 268 ATCGAT AT^CGAT
DrdI 4256 GACAAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1580 CCGTGG C^C[GA][CT]GG
EaeI 424 TGGCCA [CT]^GGCC[GA]
EcoNI 4660 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4022 GGTTACC G^GT[ACGT]ACC
EcoT22I 4735 ATGCAT ATGCA^T
FauI 4956 GCGGG CCCGC(4/6)
FbaI 2853 TGATCA T^GATCA
HaeII 2743 AGCGCC [GA]GCGC^[CT]
HgaI 4090 GACGC GACGC(5/10)
HincII 2560 GTTGAC GT[CT]^[GA]AC
HindII 2560 GTTGAC GT[CT]^[GA]AC
MfeI 2257 CAATTG C^AATTG
MluNI 424 TGGCCA TGG^CCA
MroI 51 TCCGGA T^CCGGA
MscI 424 TGGCCA TGG^CCA
MunI 2257 CAATTG C^AATTG
NciI 568 CCGGG CC^[GC]GG
NheI 894 GCTAGC G^CTAGC
NsiI 4735 ATGCAT ATGCA^T
Psp1406I 2693 AACGTT AA^CGTT
PvuII 5035 CAGCTG CAG^CTG
RcaI 1467 TCATGA T^CATGA
SapI 1690 GCTCTTC GCTCTTC(1/4)
ScaI 658 AGTACT AGT^ACT
StuI 3953 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AocI 835 CCTAAGG CC^T[ACGT]AGG
3576 CCTCAGG
AseI 3397 ATTAAT AT^TAAT
3895 ATTAAT
AsnI 3397 ATTAAT AT^TAAT
3895 ATTAAT
BpmI 2056 CTCCAG CTGGAG(16/14)
4664 CTCCAG
BsmBI 3274 CGTCTC CGTCTC(1/5)
5017 GAGACG
BspMI 603 ACCTGC ACCTGC(4/8)
4645 GCAGGT
BstBI 1022 TTCGAA TT^CGAA
3773 TTCGAA
Bsu36I 835 CCTAAGG CC^T[ACGT]AGG
3576 CCTCAGG
CfoI 1237 GCGC GCG^C
2744 GCGC
Eco81I 835 CCTAAGG CC^T[ACGT]AGG
3576 CCTCAGG
HapII 52 CCGG C^CGG
568 CCGG
HhaI 1237 GCGC GCG^C
2744 GCGC
HinP1I 1237 GCGC G^CGC
2744 GCGC
HpaII 52 CCGG C^CGG
568 CCGG
MspA1I 21 CAGCGG C[AC]G^C[GT]G
5035 CAGCTG
MspI 52 CCGG C^CGG
568 CCGG
MwoI 1552 GCCCGAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
4630 GCTAGGTCTGC
NspV 1022 TTCGAA TT^CGAA
3773 TTCGAA
PshBI 3397 ATTAAT AT^TAAT
3895 ATTAAT
SfuI 1022 TTCGAA TT^CGAA
3773 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BamHI
BanII BbsI BbvI BfaI BglII
BmyI BpuAI BsaBI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MvaI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI