Restriction map for
pF1KB5651
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbrPI BcgI BclI BfrI
BglII BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BspMI
BsrGI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SapI SbfI ScaI SexAI SfaNI
SfiI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 785 GTCGAC GT^[AC][GT]AC
AccIII 978 TCCGGA T^CCGGA
AclI 1102 AACGTT AA^CGTT
AcyI 15 GGCGCC G[GA]^CG[CT]C
Asp700I 733 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 463 CCTAGG C^CTAGG
BalI 411 TGGCCA TGG^CCA
BbeI 15 GGCGCC GGCGC^C
BbvCI 990 CCTCAGC CCTCAGC(-5/-2)
BglI 33 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 463 CCTAGG C^CTAGG
BsaHI 15 GGCGCC G[GA]^CG[CT]C
BsaWI 978 TCCGGA [AT]^CCGG[AT]
BseAI 978 TCCGGA T^CCGGA
BseRI 754 GAGGAG GAGGAG(10/8)
BsmAI 808 GAGAC GTCTC(1/5)
BsmI 743 GAATGC GAATGC(1/-1)
BspEI 978 TCCGGA T^CCGGA
BsrDI 995 GCAATG GCAATG(2/0)
BsrFI 292 ACCGGC [GA]^CCGG[CT]
BstAPI 696 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 740 TTCGAA TT^CGAA
BstF5I 1002 GGATG GGATG(2/0)
Cfr10I 292 ACCGGC [GA]^CCGG[CT]
FokI 1002 GGATG GGATG(9/13)
HaeII 15 GGCGCC [GA]GCGC^[CT]
HgaI 274 GCGTC GACGC(5/10)
Hin1I 15 GGCGCC G[GA]^CG[CT]C
KasI 15 GGCGCC G^GCGCC
MluNI 411 TGGCCA TGG^CCA
MroI 978 TCCGGA T^CCGGA
MscI 411 TGGCCA TGG^CCA
MseI 158 TTAA T^TAA
MspA1I 283 CAGCTG C[AC]G^C[GT]G
NarI 15 GGCGCC GG^CGCC
NdeI 493 CATATG CA^TATG
NspI 249 GCATGT [GA]CATG^[CT]
NspV 740 TTCGAA TT^CGAA
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1102 AACGTT AA^CGTT
PstI 280 CTGCAG CTGCA^G
PvuII 283 CAGCTG CAG^CTG
SacI 757 GAGCTC GAGCT^C
SalI 785 GTCGAC G^TCGAC
SfoI 15 GGCGCC GGC^GCC
SfuI 740 TTCGAA TT^CGAA
SgrAI 291 CACCGGCG C[GA]^CCGG[CT]G
Tru9I 158 TTAA T^TAA
XmnI 733 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 420 GTAC GT^AC
605 GTAC
AlwNI 481 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
697 CAGAAACTG
AspHI 757 GAGCTC G[AT]GC[AT]^C
930 GAGCAC
AvaI 219 CCCGGG C^[CT]CG[GA]G
381 CCCGGG
AvaII 66 GGACC G^G[AT]CC
539 GGTCC
BbsI 1129 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BciVI 1011 GGATAC GTATCC(6/5)
550 GTATCC
BpuAI 1129 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BsiHKAI 757 GAGCTC G[AT]GC[AT]^C
930 GAGCAC
BsmFI 276 GTCCC GGGAC(10/14)
967 GTCCC
BsoBI 219 CCCGGG C^[CT]CG[GA]G
381 CCCGGG
BsrBI 25 CCGCTC CCGCTC(-3/-3)
255 CCGCTC
BssSI 123 CTCGTG CACGAG(-5/-1)
565 CTCGTG
DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT]
222 GGGGCCT
DsaI 542 CCATGG C^C[GA][CT]GG
797 CCATGG
EarI 91 CTCTTC CTCTTC(1/4)
1144 CTCTTC
EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT]
222 GGGGCCT
HphI 446 TCACC GGTGA(8/7)
576 TCACC
Ksp632I 91 CTCTTC CTCTTC(1/4)
1144 CTCTTC
MaeIII 58 GTCAC ^GT[ACGT]AC
358 GTGAC
MslI 522 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
654 CATGAGTATG
NcoI 542 CCATGG C^CATGG
797 CCATGG
RsaI 420 GTAC GT^AC
605 GTAC
SfcI 280 CTGCAG C^T[GA][CT]AG
960 CTACAG
SmaI 219 CCCGGG CCC^GGG
381 CCCGGG
Tsp45I 58 GTCAC ^GT[GC]AC
358 GTGAC
XmaCI 219 CCCGGG C^CCGGG
381 CCCGGG
XmaI 219 CCCGGG C^CCGGG
381 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI BanI
BanII BbvI BcnI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BspHI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII ItaI MaeI
MaeII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RcaI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI TseI TspRI TthHB8I XhoII