Restriction map for
pF1KB4393
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII BbeI BfrI BlpI
Bpu1102I BsaHI BsiWI BspLU11I BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII CpoI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FspI Hin1I
HpaI KasI KspI MluI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 490 GGTACC G^GTACC
AccII 3282 CGCG CG^CG
AccIII 1124 TCCGGA T^CCGGA
Asp718I 490 GGTACC G^GTACC
AvrII 1962 CCTAGG C^CTAGG
BbrPI 3856 CACGTG CAC^GTG
BbvCI 2230 GCTGAGG CCTCAGC(-5/-2)
BcgI 3836 GCATGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2605 GGATAC GTATCC(6/5)
BclI 914 TGATCA T^GATCA
BglI 2050 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1962 CCTAGG C^CTAGG
BsaBI 1825 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1124 TCCGGA T^CCGGA
BsiEI 2559 CGGCCG CG[GA][CT]^CG
BsmBI 22 GAGACG CGTCTC(1/5)
BspDI 1363 ATCGAT AT^CGAT
BspEI 1124 TCCGGA T^CCGGA
BsrDI 1701 CATTGC GCAATG(2/0)
BssSI 3298 CTCGTG CACGAG(-5/-1)
BstUI 3282 CGCG CG^CG
ClaI 1363 ATCGAT AT^CGAT
DrdI 2841 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2559 CGGCCG C^GGCCG
EclXI 2559 CGGCCG C^GGCCG
Eco52I 2559 CGGCCG C^GGCCG
EcoRI 4147 GAATTC G^AATTC
FauI 3696 GCGGG CCCGC(4/6)
FbaI 914 TGATCA T^GATCA
FseI 2556 GGCCGGCC GGCCGG^CC
HaeII 478 GGCGCT [GA]GCGC^[CT]
HincII 2575 GTCGAC GT[CT]^[GA]AC
HindII 2575 GTCGAC GT[CT]^[GA]AC
HindIII 3670 AAGCTT A^AGCTT
KpnI 490 GGTACC GGTAC^C
MamI 1825 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2090 CAATTG C^AATTG
MroI 1124 TCCGGA T^CCGGA
MunI 2090 CAATTG C^AATTG
MvnI 3282 CGCG CG^CG
PaeR7I 2420 CTCGAG C^TCGAG
PmaCI 3856 CACGTG CAC^GTG
PmlI 3856 CACGTG CAC^GTG
SalI 2575 GTCGAC G^TCGAC
SapI 4074 GCTCTTC GCTCTTC(1/4)
SbfI 2895 CCTGCAGG CCTGCA^GG
ScaI 1868 AGTACT AGT^ACT
Sse8387I 2895 CCTGCAGG CCTGCA^GG
SspI 1984 AATATT AAT^ATT
StuI 1816 AGGCCT AGG^CCT
XhoI 2420 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 2065 GTCTAC GT^[AC][GT]AC
2575 GTCGAC
BamHI 558 GGATCC G^GATCC
1972 GGATCC
BsaAI 3559 TACGTG [CT]AC^GT[GA]
3856 CACGTG
Bsp1407I 122 TGTACA T^GTACA
1477 TGTACA
BsrFI 2155 GCCGGC [GA]^CCGG[CT]
2557 GCCGGC
BsrGI 122 TGTACA T^GTACA
1477 TGTACA
Cfr10I 2155 GCCGGC [GA]^CCGG[CT]
2557 GCCGGC
DraI 679 TTTAAA TTT^AAA
2761 TTTAAA
EarI 4075 CTCTTC CTCTTC(1/4)
2862 GAAGAG
EcoNI 2113 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3573 CCTGAAAGAGG
HgaI 3280 GACGC GACGC(5/10)
3988 GACGC
Ksp632I 4075 CTCTTC CTCTTC(1/4)
2862 GAAGAG
MaeII 3560 ACGT A^CGT
3857 ACGT
NaeI 2155 GCCGGC GCC^GGC
2557 GCCGGC
NcoI 2336 CCATGG C^CATGG
3821 CCATGG
NgoMIV 2155 GCCGGC G^CCGGC
2557 GCCGGC
NspI 141 ACATGC [GA]CATG^[CT]
2609 ACATGC
PleI 4012 GACTC GAGTC(4/5)
2848 GAGTC
PpuMI 2446 AGGTCCT [GA]G^G[AT]CC[CT]
4103 GGGACCC
SspBI 122 TGTACA T^GTACA
1477 TGTACA
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI