##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI BbeI BbvCI BfrI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoT22I
FseI HaeII HgaI HpaI KasI
Ksp632I KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI StuI SwaI
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1682 GACGTC GACGT^C
AccII 1181 CGCG CG^CG
AcyI 1682 GACGTC G[GA]^CG[CT]C
Alw44I 1070 GTGCAC G^TGCAC
ApaLI 1070 GTGCAC G^TGCAC
AspI 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3845 TGCGCA TGC^GCA
AvrII 2793 CCTAGG C^CTAGG
BcgI 1010 CGAGGGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2793 CCTAGG C^CTAGG
BlpI 1331 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1331 GCTCAGC GC^T[ACGT]AGC
BsaHI 1682 GACGTC G[GA]^CG[CT]C
BsaWI 4322 TCCGGT [AT]^CCGG[AT]
BsiEI 2963 CGACCG CG[GA][CT]^CG
BspLU11I 4003 ACATGT A^CATGT
BsrDI 3158 GCAATG GCAATG(2/0)
BsrFI 396 ACCGGC [GA]^CCGG[CT]
BstAPI 1894 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1181 CGCG CG^CG
CelII 1331 GCTCAGC GC^T[ACGT]AGC
Cfr10I 396 ACCGGC [GA]^CCGG[CT]
DsaI 992 CCATGG C^C[GA][CT]GG
FspI 3845 TGCGCA TGC^GCA
Hin1I 1682 GACGTC G[GA]^CG[CT]C
HincII 503 GTCAAC GT[CT]^[GA]AC
HindII 503 GTCAAC GT[CT]^[GA]AC
HindIII 3898 AAGCTT A^AGCTT
MfeI 3584 CAATTG C^AATTG
MseI 145 TTAA T^TAA
MunI 3584 CAATTG C^AATTG
MvnI 1181 CGCG CG^CG
NcoI 992 CCATGG C^CATGG
PflFI 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 3224 CTGCAG CTGCA^G
SbfI 3223 CCTGCAGG CCTGCA^GG
Sse8387I 3223 CCTGCAGG CCTGCA^GG
SspI 4312 AATATT AAT^ATT
Tru9I 145 TTAA T^TAA
Tth111I 2207 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1312 GGTACC G^GTACC
3166 GGTACC
AflIII 3883 ACGTGT A^C[GA][CT]GT
4003 ACATGT
Asp718I 1312 GGTACC G^GTACC
3166 GGTACC
BalI 132 TGGCCA TGG^CCA
3678 TGGCCA
BamHI 1674 GGATCC G^GATCC
2644 GGATCC
BbrPI 758 CACGTG CAC^GTG
3882 CACGTG
BciVI 1115 GGATAC GTATCC(6/5)
4228 GGATAC
BclI 2079 TGATCA T^GATCA
3393 TGATCA
BglII 179 AGATCT A^GATCT
4425 AGATCT
BsgI 120 CTGCAC GTGCAG(16/14)
695 GTGCAG
DraIII 1046 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1487 CACCCGGTG
EcoNI 2056 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3943 CCTAACCAAGG
EcoRI 1385 GAATTC G^AATTC
4462 GAATTC
EcoRV 3703 GATATC GAT^ATC
4294 GATATC
FbaI 2079 TGATCA T^GATCA
3393 TGATCA
KpnI 1312 GGTACC GGTAC^C
3166 GGTACC
MluNI 132 TGGCCA TGG^CCA
3678 TGGCCA
MscI 132 TGGCCA TGG^CCA
3678 TGGCCA
MslI 2848 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3715 CACTATGATG
PaeR7I 2459 CTCGAG C^TCGAG
2504 CTCGAG
PleI 2177 GACTC GAGTC(4/5)
615 GAGTC
PmaCI 758 CACGTG CAC^GTG
3882 CACGTG
PmlI 758 CACGTG CAC^GTG
3882 CACGTG
PshAI 530 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3412 GACAGAGGTC
XhoI 2459 CTCGAG C^TCGAG
2504 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI EaeI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SmaI SmlI SphI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI