Restriction map for
pF1KA2032
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AspEI AspHI AspI AvrII
BalI BamHI BbrPI BcgI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FauI
FseI HgaI HindIII HpaI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 2430 GGCGCC G[GA]^CG[CT]C
AlwNI 893 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1013 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2688 TGCGCA TGC^GCA
BbeI 2430 GGCGCC GGCGC^C
BclI 1817 TGATCA T^GATCA
BglII 1770 AGATCT A^GATCT
BlpI 2746 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2746 GCTCAGC GC^T[ACGT]AGC
BsaHI 2430 GGCGCC G[GA]^CG[CT]C
BsmBI 1625 CGTCTC CGTCTC(1/5)
BsmI 2518 GCATTC GAATGC(1/-1)
Bsp1407I 1668 TGTACA T^GTACA
BsrBI 2540 CCGCTC CCGCTC(-3/-3)
BsrGI 1668 TGTACA T^GTACA
BstBI 744 TTCGAA TT^CGAA
BstYI 1770 AGATCT [GA]^GATC[CT]
CelII 2746 GCTCAGC GC^T[ACGT]AGC
DraI 2007 TTTAAA TTT^AAA
EcoNI 1138 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 405 GATATC GAT^ATC
EcoT14I 1320 CCAAGG C^C[AT][AT]GG
FbaI 1817 TGATCA T^GATCA
FspI 2688 TGCGCA TGC^GCA
Hin1I 2430 GGCGCC G[GA]^CG[CT]C
HincII 794 GTCAAC GT[CT]^[GA]AC
HindII 794 GTCAAC GT[CT]^[GA]AC
HphI 502 GGTGA GGTGA(8/7)
KasI 2430 GGCGCC G^GCGCC
MaeII 1705 ACGT A^CGT
MfeI 74 CAATTG C^AATTG
MflI 1770 AGATCT [GA]^GATC[CT]
MslI 1704 CACGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 74 CAATTG C^AATTG
NarI 2430 GGCGCC GG^CGCC
NspI 624 ACATGC [GA]CATG^[CT]
NspV 744 TTCGAA TT^CGAA
PflMI 2044 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2336 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1999 AGTACT AGT^ACT
SfoI 2430 GGCGCC GGC^GCC
SfuI 744 TTCGAA TT^CGAA
SpeI 249 ACTAGT A^CTAGT
SspBI 1668 TGTACA T^GTACA
StyI 1320 CCAAGG C^C[AT][AT]GG
Van91I 2044 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1592 CCACTCCAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1770 AGATCT [GA]^GATC[CT]
XmnI 1013 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 666 GGTACC G^GTACC
1762 GGTACC
AseI 874 ATTAAT AT^TAAT
2172 ATTAAT
AsnI 874 ATTAAT AT^TAAT
2172 ATTAAT
Asp718I 666 GGTACC G^GTACC
1762 GGTACC
AvaI 2418 CCCGGG C^[CT]CG[GA]G
2619 CCCGGG
BbsI 449 GAAGAC GAAGAC(2/6)
736 GAAGAC
BciVI 91 GGATAC GTATCC(6/5)
2624 GTATCC
BpuAI 449 GAAGAC GAAGAC(2/6)
736 GAAGAC
BsaI 957 GGTCTC GGTCTC(1/5)
1984 GGTCTC
BsoBI 2418 CCCGGG C^[CT]CG[GA]G
2619 CCCGGG
BsrFI 1375 GCCGGT [GA]^CCGG[CT]
2557 GCCGGT
BstAPI 628 GCAACTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
637 GCACCAAGTGC
Cfr10I 1375 GCCGGT [GA]^CCGG[CT]
2557 GCCGGT
DpnII 1771 GATC ^GATC
1818 GATC
DraIII 638 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1382 CACTTGGTG
DsaI 1156 CCACGG C^C[GA][CT]GG
1631 CCGTGG
EcoRI 294 GAATTC G^AATTC
542 GAATTC
HaeII 2430 GGCGCC [GA]GCGC^[CT]
2778 AGCGCC
KpnI 666 GGTACC GGTAC^C
1762 GGTACC
MboI 1771 GATC ^GATC
1818 GATC
NdeII 1771 GATC ^GATC
1818 GATC
PleI 1338 GACTC GAGTC(4/5)
1525 GACTC
PshBI 874 ATTAAT AT^TAAT
2172 ATTAAT
PstI 2425 CTGCAG CTGCA^G
2582 CTGCAG
Sau3AI 1771 GATC ^GATC
1818 GATC
SmaI 2418 CCCGGG CCC^GGG
2619 CCCGGG
SmlI 1995 CTTGAG C^T[CT][GA]AG
2330 CTCAAG
TaqI 274 TCGA T^CGA
745 TCGA
TthHB8I 274 TCGA T^CGA
745 TCGA
XmaCI 2418 CCCGGG C^CCGGG
2619 CCCGGG
XmaI 2418 CCCGGG C^CCGGG
2619 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PpuMI PvuII
RsaI SapI Sau96I ScrFI SfaNI
SfcI StuI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI