Restriction map for
pF1KA2010
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaI BsiWI BsmBI BsmI
BsoBI BspDI BspHI BspMI BsrDI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FbaI
FseI FspI HaeII HgaI HhaI
HinP1I KasI KpnI KspI MfeI
MluI MluNI MscI MspA1I MunI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SwaI
Tsp45I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 655 GACGTC GACGT^C
AccIII 1571 TCCGGA T^CCGGA
AcyI 655 GACGTC G[GA]^CG[CT]C
AflIII 851 ACATGT A^C[GA][CT]GT
AseI 1555 ATTAAT AT^TAAT
AsnI 1555 ATTAAT AT^TAAT
AspHI 1522 GAGCAC G[AT]GC[AT]^C
AspI 1065 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 67 GGCACC G^G[CT][GA]CC
BanII 2181 GAGCCC G[GA]GC[CT]^C
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsaBI 1270 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 655 GACGTC G[GA]^CG[CT]C
BsaWI 1571 TCCGGA [AT]^CCGG[AT]
BseAI 1571 TCCGGA T^CCGGA
BsgI 565 CTGCAC GTGCAG(16/14)
BsiEI 60 CGACCG CG[GA][CT]^CG
BsiHKAI 1522 GAGCAC G[AT]GC[AT]^C
BsmAI 2052 GTCTC GTCTC(1/5)
BspEI 1571 TCCGGA T^CCGGA
BspLU11I 851 ACATGT A^CATGT
BsrBI 97 GAGCGG CCGCTC(-3/-3)
BsrFI 47 ACCGGC [GA]^CCGG[CT]
BssSI 2359 CTCGTG CACGAG(-5/-1)
BstBI 1316 TTCGAA TT^CGAA
Cfr10I 47 ACCGGC [GA]^CCGG[CT]
EcoT22I 1405 ATGCAT ATGCA^T
Hin1I 655 GACGTC G[GA]^CG[CT]C
HindIII 2194 AAGCTT A^AGCTT
MaeIII 2427 GTTAC ^GT[ACGT]AC
MamI 1270 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1571 TCCGGA T^CCGGA
NcoI 1181 CCATGG C^CATGG
NdeI 1258 CATATG CA^TATG
NheI 1344 GCTAGC G^CTAGC
NsiI 1405 ATGCAT ATGCA^T
NspV 1316 TTCGAA TT^CGAA
PflFI 1065 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 157 GAGTC GAGTC(4/5)
PshBI 1555 ATTAAT AT^TAAT
SfuI 1316 TTCGAA TT^CGAA
SphI 1602 GCATGC GCATG^C
SspI 553 AATATT AAT^ATT
StuI 2355 AGGCCT AGG^CCT
Tth111I 1065 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 758 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1274 CAGATCCTG
BbsI 1444 GAAGAC GAAGAC(2/6)
2217 GAAGAC
BbvI 2335 GCAGC GCAGC(8/12)
1138 GCTGC
BpmI 2291 CTCCAG CTGGAG(16/14)
520 CTGGAG
BpuAI 1444 GAAGAC GAAGAC(2/6)
2217 GAAGAC
BseRI 355 GAGGAG GAGGAG(10/8)
1289 GAGGAG
BsmFI 56 GGGAC GGGAC(10/14)
114 GTCCC
Bsp1407I 29 TGTACA T^GTACA
1860 TGTACA
BsrGI 29 TGTACA T^GTACA
1860 TGTACA
BstAPI 1398 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1615 GCATTATGTGC
DraII 308 AGGACCC [GA]G^G[ACGT]CC[CT]
332 AGGACCT
DsaI 317 CCGTGG C^C[GA][CT]GG
1181 CCATGG
EcoO109I 308 AGGACCC [GA]G^G[ACGT]CC[CT]
332 AGGACCT
EcoRV 1564 GATATC GAT^ATC
1961 GATATC
EcoT14I 1107 CCTTGG C^C[AT][AT]GG
1181 CCATGG
FauI 1736 CCCGC CCCGC(4/6)
18 GCGGG
HpaI 994 GTTAAC GTT^AAC
1506 GTTAAC
MslI 532 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
571 CACTTGTATG
PflMI 1299 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1479 CCAACTATTGG
PpuMI 308 AGGACCC [GA]G^G[AT]CC[CT]
332 AGGACCT
SfcI 1033 CTACAG C^T[GA][CT]AG
2045 CTGTAG
SspBI 29 TGTACA T^GTACA
1860 TGTACA
StyI 1107 CCTTGG C^C[AT][AT]GG
1181 CCATGG
TseI 1138 GCTGC G^C[AT]GC
2335 GCAGC
Van91I 1299 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1479 CCAACTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BamHI BcnI
BfaI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
XhoII XmnI