Restriction map for
pF1KA1975
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# NO CUT ENZYMES ( 123 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI HgaI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1537 GACGTC GACGT^C
AcyI 1537 GACGTC G[GA]^CG[CT]C
Alw44I 112 GTGCAC G^TGCAC
Aor51HI 1569 AGCGCT AGC^GCT
ApaLI 112 GTGCAC G^TGCAC
AvaI 1547 CCCGAG C^[CT]CG[GA]G
AviII 571 TGCGCA TGC^GCA
BamHI 1289 GGATCC G^GATCC
BanI 1120 GGCACC G^G[CT][GA]CC
BanII 1171 GAGCTC G[GA]GC[CT]^C
BbsI 408 GAAGAC GAAGAC(2/6)
BbvCI 802 GCTGAGG CCTCAGC(-5/-2)
BcnI 1583 CCCGG CC^[GC]GG
BglII 61 AGATCT A^GATCT
BpuAI 408 GAAGAC GAAGAC(2/6)
BsaHI 1537 GACGTC G[GA]^CG[CT]C
BsmBI 1457 GAGACG CGTCTC(1/5)
BsoBI 1547 CCCGAG C^[CT]CG[GA]G
BstAPI 1359 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 1273 GAAGAG CTCTTC(1/4)
Eco47III 1569 AGCGCT AGC^GCT
EcoNI 603 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 646 GATATC GAT^ATC
FspI 571 TGCGCA TGC^GCA
HapII 1584 CCGG C^CGG
Hin1I 1537 GACGTC G[GA]^CG[CT]C
HincII 1263 GTCAAC GT[CT]^[GA]AC
HindII 1263 GTCAAC GT[CT]^[GA]AC
HindIII 468 AAGCTT A^AGCTT
HpaII 1584 CCGG C^CGG
Ksp632I 1273 GAAGAG CTCTTC(1/4)
MslI 1608 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1584 CCGG C^CGG
NciI 1583 CCCGG CC^[GC]GG
PvuII 961 CAGCTG CAG^CTG
SacI 1171 GAGCTC GAGCT^C
SphI 377 GCATGC GCATG^C
SspI 490 AATATT AAT^ATT
StuI 1589 AGGCCT AGG^CCT
TaqI 997 TCGA T^CGA
Tsp509I 640 AATT ^AATT
TthHB8I 997 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 1527 GTAC GT^AC
1626 GTAC
BfaI 556 CTAG C^TAG
1207 CTAG
BglI 559 GCCACATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1582 GCCCGGCAGGC
BsaI 1039 GAGACC GGTCTC(1/5)
1441 GAGACC
BsgI 1335 CTGCAC GTGCAG(16/14)
1464 CTGCAC
BsrBI 348 GAGCGG CCGCTC(-3/-3)
880 GAGCGG
BsrDI 596 GCAATG GCAATG(2/0)
1199 GCAATG
BstXI 538 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
608 CCAAGGACATGG
DraI 147 TTTAAA TTT^AAA
859 TTTAAA
DraII 197 AGGACCT [GA]G^G[ACGT]CC[CT]
1385 AGGACCT
DraIII 432 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1024 CACTGCGTG
EcoO109I 197 AGGACCT [GA]G^G[ACGT]CC[CT]
1385 AGGACCT
HaeII 1470 GGCGCT [GA]GCGC^[CT]
1569 AGCGCT
MaeI 556 CTAG C^TAG
1207 CTAG
MspA1I 961 CAGCTG C[AC]G^C[GT]G
1376 CCGCTG
NcoI 257 CCATGG C^CATGG
983 CCATGG
NdeI 628 CATATG CA^TATG
666 CATATG
NspI 377 GCATGC [GA]CATG^[CT]
1007 ACATGC
PpuMI 197 AGGACCT [GA]G^G[AT]CC[CT]
1385 AGGACCT
RsaI 1527 GTAC GT^AC
1626 GTAC
TfiI 1047 GAATC G^A[AT]TC
1103 GAATC
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BalI BbvI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
Tru9I TseI Tsp45I TspRI Van91I
XcmI XhoII