Restriction map for
pF1KA1867
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmI
BsoBI BspDI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I CelII ClaI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
SacI SacII SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tru9I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 1176 CGCG CG^CG
AclI 1372 AACGTT AA^CGTT
AcsI 1819 GAATTC [GA]^AATT[CT]
AocI 342 CCTGAGG CC^T[ACGT]AGG
ApoI 1819 GAATTC [GA]^AATT[CT]
BbsI 1140 GAAGAC GAAGAC(2/6)
BclI 470 TGATCA T^GATCA
BpuAI 1140 GAAGAC GAAGAC(2/6)
BsaI 1411 GAGACC GGTCTC(1/5)
Bsp1407I 893 TGTACA T^GTACA
BsrDI 1133 GCAATG GCAATG(2/0)
BsrFI 1075 ACCGGC [GA]^CCGG[CT]
BsrGI 893 TGTACA T^GTACA
BssSI 1033 CTCGTG CACGAG(-5/-1)
BstUI 1176 CGCG CG^CG
BstXI 950 CCAACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1075 ACCGGC [GA]^CCGG[CT]
CpoI 1343 CGGACCG CG^G[AT]CCG
DraIII 2172 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1293 CCACGG C^C[GA][CT]GG
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
EcoRI 1819 GAATTC G^AATTC
EcoRV 1726 GATATC GAT^ATC
EcoT14I 104 CCAAGG C^C[AT][AT]GG
FbaI 470 TGATCA T^GATCA
HaeII 1397 GGCGCT [GA]GCGC^[CT]
MvnI 1176 CGCG CG^CG
NspI 2015 GCATGT [GA]CATG^[CT]
Psp1406I 1372 AACGTT AA^CGTT
RsrII 1343 CGGACCG CG^G[AT]CCG
SalI 2300 GTCGAC G^TCGAC
SapI 24 GCTCTTC GCTCTTC(1/4)
SfiI 384 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 893 TGTACA T^GTACA
StyI 104 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 717 GACGTC GACGT^C
988 GACGTC
AccI 991 GTCTAC GT^[AC][GT]AC
2300 GTCGAC
AccIII 1117 TCCGGA T^CCGGA
1325 TCCGGA
AflIII 914 ACACGT A^C[GA][CT]GT
1187 ACGTGT
ApaI 462 GGGCCC GGGCC^C
1000 GGGCCC
Asp700I 3 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1506 GAACAGCTTC
BalI 101 TGGCCA TGG^CCA
663 TGGCCA
BanI 432 GGTGCC G^G[CT][GA]CC
1203 GGTGCC
BbvCI 972 CCTCAGC CCTCAGC(-5/-2)
2145 CCTCAGC
BglI 385 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2236 GCCAGCAAGGC
BsaAI 915 CACGTA [CT]AC^GT[GA]
1186 TACGTG
BsaWI 1117 TCCGGA [AT]^CCGG[AT]
1325 TCCGGA
BseAI 1117 TCCGGA T^CCGGA
1325 TCCGGA
BsgI 1494 CTGCAC GTGCAG(16/14)
2221 GTGCAG
BsmBI 933 CGTCTC CGTCTC(1/5)
715 GAGACG
BspEI 1117 TCCGGA T^CCGGA
1325 TCCGGA
BspMI 505 ACCTGC ACCTGC(4/8)
1979 ACCTGC
BstAPI 514 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1998 GCAGCGTGTGC
BstYI 338 AGATCC [GA]^GATC[CT]
1038 GGATCT
DrdI 790 GACAACGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2182 GACAGCAGCGTC
MflI 338 AGATCC [GA]^GATC[CT]
1038 GGATCT
MluNI 101 TGGCCA TGG^CCA
663 TGGCCA
MroI 1117 TCCGGA T^CCGGA
1325 TCCGGA
MscI 101 TGGCCA TGG^CCA
663 TGGCCA
MslI 1611 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2010 CACAGGCATG
PstI 530 CTGCAG CTGCA^G
2308 CTGCAG
SexAI 167 ACCAGGT A^CC[AT]GGT
296 ACCTGGT
SmlI 279 CTCAAG C^T[CT][GA]AG
1540 CTCAAG
Tsp509I 1740 AATT ^AATT
1820 AATT
XhoII 338 AGATCC [GA]^GATC[CT]
1038 GGATCT
XmnI 3 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1506 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AspI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstEII BstF5I BstNI BstPI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoO65I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PshAI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I Van91I