Restriction map for
pF1KA1745
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AscI
AseI AsnI AspEI AspI BbeI
BclI BfrI BsaBI BsaHI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 2357 GGTACC G^GTACC
AocI 21 CCTGAGG CC^T[ACGT]AGG
Aor51HI 893 AGCGCT AGC^GCT
Asp718I 2357 GGTACC G^GTACC
AviII 5 TGCGCA TGC^GCA
AvrII 2322 CCTAGG C^CTAGG
BamHI 1694 GGATCC G^GATCC
BbrPI 1906 CACGTG CAC^GTG
BcgI 2336 GCACCCCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2449 GTATCC GTATCC(6/5)
BglI 57 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2322 CCTAGG C^CTAGG
BsaI 2302 GAGACC GGTCTC(1/5)
BsaWI 2219 ACCGGA [AT]^CCGG[AT]
BsiEI 1410 CGGCCG CG[GA][CT]^CG
BsrDI 843 CATTGC GCAATG(2/0)
BssHII 50 GCGCGC G^CGCGC
BstAPI 1494 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 184 TTCGAA TT^CGAA
BstXI 578 CCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 21 CCTGAGG CC^T[ACGT]AGG
DraIII 1165 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1288 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1410 CGGCCG C^GGCCG
EclXI 1410 CGGCCG C^GGCCG
Eco47III 893 AGCGCT AGC^GCT
Eco52I 1410 CGGCCG C^GGCCG
Eco81I 21 CCTGAGG CC^T[ACGT]AGG
FspI 5 TGCGCA TGC^GCA
KpnI 2357 GGTACC GGTAC^C
NaeI 1412 GCCGGC GCC^GGC
NgoMIV 1412 GCCGGC G^CCGGC
NspI 1307 GCATGC [GA]CATG^[CT]
NspV 184 TTCGAA TT^CGAA
PaeR7I 257 CTCGAG C^TCGAG
PmaCI 1906 CACGTG CAC^GTG
PmlI 1906 CACGTG CAC^GTG
SapI 163 GAAGAGC GCTCTTC(1/4)
SfuI 184 TTCGAA TT^CGAA
SphI 1307 GCATGC GCATG^C
XcmI 578 CCACTGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 257 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 567 GAATTT [GA]^AATT[CT]
808 GAATTT
ApoI 567 GAATTT [GA]^AATT[CT]
808 GAATTT
Asp700I 2145 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2232 GAAAGTCTTC
BglII 776 AGATCT A^GATCT
1469 AGATCT
BpmI 1641 CTCCAG CTGGAG(16/14)
1187 CTGGAG
BsaAI 247 TACGTG [CT]AC^GT[GA]
1906 CACGTG
Bsp1407I 1350 TGTACA T^GTACA
1583 TGTACA
BsrFI 1412 GCCGGC [GA]^CCGG[CT]
2213 ACCGGC
BsrGI 1350 TGTACA T^GTACA
1583 TGTACA
Cfr10I 1412 GCCGGC [GA]^CCGG[CT]
2213 ACCGGC
EcoNI 1116 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2241 CCTGAAGCAGG
NcoI 43 CCATGG C^CATGG
1565 CCATGG
SacI 601 GAGCTC GAGCT^C
687 GAGCTC
SspBI 1350 TGTACA T^GTACA
1583 TGTACA
TfiI 182 GATTC G^A[AT]TC
1497 GAATC
Tsp509I 568 AATT ^AATT
809 AATT
XmnI 2145 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2232 GAAAGTCTTC
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI