Restriction map for
pF1KA1645
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI ScaI SexAI SfuI
SmlI SnaBI SpeI SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 145 GTAC GT^AC
AgeI 63 ACCGGT A^CCGGT
Aor51HI 304 AGCGCT AGC^GCT
AvaI 760 CCCGGG C^[CT]CG[GA]G
BcgI 602 GCAGCCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 128 GGTCTC GGTCTC(1/5)
BsaWI 63 ACCGGT [AT]^CCGG[AT]
BsmBI 582 CGTCTC CGTCTC(1/5)
BsoBI 760 CCCGGG C^[CT]CG[GA]G
BspHI 744 TCATGA T^CATGA
BspMI 729 GCAGGT ACCTGC(4/8)
BstEII 177 GGTTACC G^GT[ACGT]ACC
BstPI 177 GGTTACC G^GT[ACGT]ACC
BstYI 797 AGATCC [GA]^GATC[CT]
EarI 118 CTCTTC CTCTTC(1/4)
Eco47III 304 AGCGCT AGC^GCT
EcoO65I 177 GGTTACC G^GT[ACGT]ACC
EcoT14I 938 CCAAGG C^C[AT][AT]GG
Ksp632I 118 CTCTTC CTCTTC(1/4)
MboII 119 TCTTC GAAGA(8/7)
MflI 797 AGATCC [GA]^GATC[CT]
NaeI 547 GCCGGC GCC^GGC
NgoMIV 547 GCCGGC G^CCGGC
PflMI 894 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 63 ACCGGT A^CCGGT
RcaI 744 TCATGA T^CATGA
RsaI 145 GTAC GT^AC
SbfI 726 CCTGCAGG CCTGCA^GG
SgrAI 62 CACCGGTG C[GA]^CCGG[CT]G
SmaI 760 CCCGGG CCC^GGG
Sse8387I 726 CCTGCAGG CCTGCA^GG
StyI 938 CCAAGG C^C[AT][AT]GG
TfiI 423 GATTC G^A[AT]TC
Van91I 894 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 797 AGATCC [GA]^GATC[CT]
XmaCI 760 CCCGGG C^CCGGG
XmaI 760 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 444 GACGTC GACGT^C
580 GACGTC
BalI 192 TGGCCA TGG^CCA
803 TGGCCA
BbeI 107 GGCGCC GGCGC^C
239 GGCGCC
BsgI 76 CTGCAC GTGCAG(16/14)
600 GTGCAG
BsmAI 129 GTCTC GTCTC(1/5)
583 GTCTC
BsrBI 112 CCGCTC CCGCTC(-3/-3)
173 GAGCGG
BsrFI 63 ACCGGT [GA]^CCGG[CT]
547 GCCGGC
Cfr10I 63 ACCGGT [GA]^CCGG[CT]
547 GCCGGC
EcoNI 249 CCTCAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
288 CCTCGCGGAGG
KasI 107 GGCGCC G^GCGCC
239 GGCGCC
KspI 680 CCGCGG CCGC^GG
929 CCGCGG
MaeII 445 ACGT A^CGT
581 ACGT
MluNI 192 TGGCCA TGG^CCA
803 TGGCCA
MscI 192 TGGCCA TGG^CCA
803 TGGCCA
MslI 489 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
828 CATCCGCGTG
NarI 107 GGCGCC GG^CGCC
239 GGCGCC
NspI 69 GCATGC [GA]CATG^[CT]
488 GCATGC
PpuMI 263 GGGACCT [GA]G^G[AT]CC[CT]
284 GGGACCT
SacII 680 CCGCGG CCGC^GG
929 CCGCGG
SfiI 193 GGCCACGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
864 GGCCGTGCTGGCC
SfoI 107 GGCGCC GGC^GCC
239 GGCGCC
SphI 69 GCATGC GCATG^C
488 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI