Restriction map for
pF1KA1622
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BseRI
BsgI BsiEI BsiWI BsmI BsoBI
BspDI BsrBI BssHII BssSI Bst1107I
BstEII BstPI BstYI BstZ17I Bsu36I
CelII CfoI ClaI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HaeII
HhaI Hin1I HinP1I KasI KpnI
KspI MflI MluI MluNI MscI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI SacII
SalI SapI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1392 GTCTAC GT^[AC][GT]AC
AccII 24 CGCG CG^CG
AccIII 2459 TCCGGA T^CCGGA
AflIII 1542 ACATGT A^C[GA][CT]GT
AhdI 1688 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1463 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1688 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 76 GAGCTC G[GA]GC[CT]^C
BbsI 151 GAAGAC GAAGAC(2/6)
BcgI 1642 CGAACTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1761 GGATAC GTATCC(6/5)
BpuAI 151 GAAGAC GAAGAC(2/6)
BsaBI 1921 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2459 TCCGGA T^CCGGA
BsmBI 262 GAGACG CGTCTC(1/5)
Bsp1407I 1030 TGTACA T^GTACA
BspEI 2459 TCCGGA T^CCGGA
BspLU11I 1542 ACATGT A^CATGT
BsrDI 449 GCAATG GCAATG(2/0)
BsrFI 96 GCCGGT [GA]^CCGG[CT]
BsrGI 1030 TGTACA T^GTACA
BstAPI 2220 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2312 TTCGAA TT^CGAA
BstUI 24 CGCG CG^CG
BstXI 720 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 96 GCCGGT [GA]^CCGG[CT]
CpoI 98 CGGTCCG CG^G[AT]CCG
DraI 1491 TTTAAA TTT^AAA
DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 436 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1145 CGGCCA [CT]^GGCC[GA]
Eam1105I 1688 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1089 CCTAGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1281 GAATTC G^AATTC
FauI 16 CCCGC CCCGC(4/6)
HgaI 264 GACGC GACGC(5/10)
HpaI 327 GTTAAC GTT^AAC
MaeII 2196 ACGT A^CGT
MamI 1921 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1717 CAATTG C^AATTG
MroI 2459 TCCGGA T^CCGGA
MslI 1593 CACAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1463 CAGCTG C[AC]G^C[GT]G
MunI 1717 CAATTG C^AATTG
MvnI 24 CGCG CG^CG
NspV 2312 TTCGAA TT^CGAA
PleI 2463 GACTC GAGTC(4/5)
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 856 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1463 CAGCTG CAG^CTG
RsrII 98 CGGTCCG CG^G[AT]CCG
SacI 76 GAGCTC GAGCT^C
SbfI 69 CCTGCAGG CCTGCA^GG
ScaI 2518 AGTACT AGT^ACT
SfuI 2312 TTCGAA TT^CGAA
Sse8387I 69 CCTGCAGG CCTGCA^GG
SspBI 1030 TGTACA T^GTACA
SspI 1811 AATATT AAT^ATT
Tsp45I 2269 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 765 ATTAAT AT^TAAT
1707 ATTAAT
AsnI 765 ATTAAT AT^TAAT
1707 ATTAAT
BsaWI 120 ACCGGA [AT]^CCGG[AT]
2459 TCCGGA
BspHI 1506 TCATGA T^CATGA
1598 TCATGA
BspMI 68 ACCTGC ACCTGC(4/8)
1824 ACCTGC
BssKI 720 CCAGG ^CC[ACGT]GG
2254 CCTGG
BstNI 720 CCAGG CC^[AT]GG
2254 CCTGG
DsaI 2526 CCGTGG C^C[GA][CT]GG
2581 CCACGG
EarI 467 CTCTTC CTCTTC(1/4)
2262 CTCTTC
EcoRII 720 CCAGG ^CC[AT]GG
2254 CCTGG
HincII 327 GTTAAC GT[CT]^[GA]AC
2545 GTTGAC
HindII 327 GTTAAC GT[CT]^[GA]AC
2545 GTTGAC
HindIII 1273 AAGCTT A^AGCTT
2336 AAGCTT
HphI 80 TCACC GGTGA(8/7)
2270 TCACC
Ksp632I 467 CTCTTC CTCTTC(1/4)
2262 CTCTTC
MvaI 720 CCAGG CC^[AT]GG
2254 CCTGG
NlaIV 107 GGAGCC GG[ACGT]^[ACGT]CC
2257 GGACCC
NspI 303 GCATGT [GA]CATG^[CT]
1542 ACATGT
PshBI 765 ATTAAT AT^TAAT
1707 ATTAAT
RcaI 1506 TCATGA T^CATGA
1598 TCATGA
ScrFI 720 CCAGG CC^[ACGT]GG
2254 CCTGG
SmlI 112 CTCAAG C^T[CT][GA]AG
999 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BstF5I Cac8I Cfr13I DdeI
DpnII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MwoI NdeII NlaIII
NsiI PstI RsaI Sau3AI Sau96I
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI