Restriction map for
pF1KA1507
##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI BbrPI BcgI BciVI
BfrI BglII BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspMI BsrGI BssHII
BstAPI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SfiI SgrAI SnaBI SpeI SspBI
SspI SwaI TfiI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 1712 CGCG CG^CG
AccIII 1728 TCCGGA T^CCGGA
AflIII 1212 ACATGT A^C[GA][CT]GT
AhdI 991 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 290 GGGCCC GGGCC^C
Asp700I 1497 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 991 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 770 TGCGCA TGC^GCA
BbeI 750 GGCGCC GGCGC^C
BbsI 727 GAAGAC GAAGAC(2/6)
BclI 2330 TGATCA T^GATCA
BglI 1785 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 727 GAAGAC GAAGAC(2/6)
BseAI 1728 TCCGGA T^CCGGA
BspDI 1960 ATCGAT AT^CGAT
BspEI 1728 TCCGGA T^CCGGA
BspHI 2439 TCATGA T^CATGA
BspLU11I 1212 ACATGT A^CATGT
BsrBI 1786 CCGCTC CCGCTC(-3/-3)
BsrFI 1881 GCCGGC [GA]^CCGG[CT]
BssSI 616 CTCGTG CACGAG(-5/-1)
Bst1107I 2425 GTATAC GTA^TAC
BstBI 1577 TTCGAA TT^CGAA
BstEII 2500 GGTGACC G^GT[ACGT]ACC
BstPI 2500 GGTGACC G^GT[ACGT]ACC
BstUI 1712 CGCG CG^CG
BstZ17I 2425 GTATAC GTA^TAC
Cfr10I 1881 GCCGGC [GA]^CCGG[CT]
ClaI 1960 ATCGAT AT^CGAT
DraIII 2495 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 991 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2500 GGTGACC G^GT[ACGT]ACC
EcoT22I 2375 ATGCAT ATGCA^T
FbaI 2330 TGATCA T^GATCA
FspI 770 TGCGCA TGC^GCA
HaeII 750 GGCGCC [GA]GCGC^[CT]
KasI 750 GGCGCC G^GCGCC
MroI 1728 TCCGGA T^CCGGA
MvnI 1712 CGCG CG^CG
NaeI 1881 GCCGGC GCC^GGC
NarI 750 GGCGCC GG^CGCC
NcoI 2633 CCATGG C^CATGG
NgoMIV 1881 GCCGGC G^CCGGC
NsiI 2375 ATGCAT ATGCA^T
NspV 1577 TTCGAA TT^CGAA
PaeR7I 2147 CTCGAG C^TCGAG
RcaI 2439 TCATGA T^CATGA
SalI 1357 GTCGAC G^TCGAC
SapI 2133 GAAGAGC GCTCTTC(1/4)
SbfI 1298 CCTGCAGG CCTGCA^GG
ScaI 1664 AGTACT AGT^ACT
SfoI 750 GGCGCC GGC^GCC
SfuI 1577 TTCGAA TT^CGAA
Sse8387I 1298 CCTGCAGG CCTGCA^GG
XhoI 2147 CTCGAG C^TCGAG
XmnI 1497 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 778 GACGTC GACGT^C
2256 GACGTC
AccI 1357 GTCGAC GT^[AC][GT]AC
2425 GTATAC
AcsI 321 GAATTT [GA]^AATT[CT]
911 AAATTT
ApoI 321 GAATTT [GA]^AATT[CT]
911 AAATTT
AvrII 1803 CCTAGG C^CTAGG
2461 CCTAGG
BalI 74 TGGCCA TGG^CCA
2294 TGGCCA
BamHI 1262 GGATCC G^GATCC
2490 GGATCC
BlnI 1803 CCTAGG C^CTAGG
2461 CCTAGG
BlpI 1549 GCTGAGC GC^T[ACGT]AGC
1974 GCTGAGC
Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC
1974 GCTGAGC
BsaI 1749 GAGACC GGTCTC(1/5)
998 GGTCTC
BsaWI 1394 ACCGGA [AT]^CCGG[AT]
1728 TCCGGA
BsgI 2017 CTGCAC GTGCAG(16/14)
1615 GTGCAG
BsmI 1580 GAATGC GAATGC(1/-1)
15 GCATTC
BsrDI 78 CATTGC GCAATG(2/0)
2318 GCAATG
BstYI 1262 GGATCC [GA]^GATC[CT]
2490 GGATCC
CelII 1549 GCTGAGC GC^T[ACGT]AGC
1974 GCTGAGC
DrdI 684 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
730 GACAGCGTGGTC
EcoNI 1218 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1436 CCTACAGAAGG
MflI 1262 GGATCC [GA]^GATC[CT]
2490 GGATCC
MluNI 74 TGGCCA TGG^CCA
2294 TGGCCA
MscI 74 TGGCCA TGG^CCA
2294 TGGCCA
MseI 1016 TTAA T^TAA
1183 TTAA
PflMI 2193 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2211 CCACATCCTGG
SexAI 227 ACCAGGT A^CC[AT]GGT
2496 ACCTGGT
SmlI 1650 CTTGAG C^T[CT][GA]AG
2147 CTCGAG
SphI 1979 GCATGC GCATG^C
2373 GCATGC
Tru9I 1016 TTAA T^TAA
1183 TTAA
Van91I 2193 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2211 CCACATCCTGG
XhoII 1262 GGATCC [GA]^GATC[CT]
2490 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI