Restriction map for
pF1KA1489
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII CfoI
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MflI MroI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 501 CGCG CG^CG
AclI 353 AACGTT AA^CGTT
AgeI 1676 ACCGGT A^CCGGT
AhdI 1675 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 626 GATCC GGATC(4/5)
AlwNI 1487 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 219 CCTGAGG CC^T[ACGT]AGG
Asp700I 574 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1675 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 469 GAGCAC G[AT]GC[AT]^C
BalI 1355 TGGCCA TGG^CCA
BsaI 1682 GGTCTC GGTCTC(1/5)
BsaWI 1676 ACCGGT [AT]^CCGG[AT]
BseRI 1157 GAGGAG GAGGAG(10/8)
BsgI 1265 GTGCAG GTGCAG(16/14)
BsiHKAI 469 GAGCAC G[AT]GC[AT]^C
BsiYI 775 CCAAGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 775 CCAAGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 213 TGTACA T^GTACA
BspHI 1281 TCATGA T^CATGA
BspLU11I 151 ACATGT A^CATGT
BsrFI 1676 ACCGGT [GA]^CCGG[CT]
BsrGI 213 TGTACA T^GTACA
BstUI 501 CGCG CG^CG
Bsu36I 219 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1676 ACCGGT [GA]^CCGG[CT]
DraI 555 TTTAAA TTT^AAA
DraII 1375 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1368 GACTAGTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1783 CCATGG C^C[GA][CT]GG
EaeI 1355 TGGCCA [CT]^GGCC[GA]
Eam1105I 1675 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 219 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1375 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 121 GAATTC G^AATTC
EcoRV 949 GATATC GAT^ATC
EcoT22I 263 ATGCAT ATGCA^T
HgaI 499 GACGC GACGC(5/10)
MfeI 1151 CAATTG C^AATTG
MluI 500 ACGCGT A^CGCGT
MluNI 1355 TGGCCA TGG^CCA
MscI 1355 TGGCCA TGG^CCA
MunI 1151 CAATTG C^AATTG
MvnI 501 CGCG CG^CG
NcoI 1783 CCATGG C^CATGG
NlaIV 925 GGGACC GG[ACGT]^[ACGT]CC
NsiI 263 ATGCAT ATGCA^T
NspI 151 ACATGT [GA]CATG^[CT]
PacI 370 TTAATTAA TTAAT^TAA
PflMI 775 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1676 ACCGGT A^CCGGT
PpuMI 1375 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 353 AACGTT AA^CGTT
PstI 1391 CTGCAG CTGCA^G
RcaI 1281 TCATGA T^CATGA
SexAI 1075 ACCTGGT A^CC[AT]GGT
SmlI 8 CTCAAG C^T[CT][GA]AG
SpeI 1369 ACTAGT A^CTAGT
SspBI 213 TGTACA T^GTACA
SspI 365 AATATT AAT^ATT
SwaI 554 ATTTAAAT ATTT^AAAT
TfiI 619 GAATC G^A[AT]TC
Van91I 775 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 574 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 1107 CCGC CCGC(-3/-1)
1689 GCGG
BbsI 639 GTCTTC GAAGAC(2/6)
1482 GTCTTC
BfaI 604 CTAG C^TAG
1370 CTAG
BmyI 114 GGGCAC G[AGT]GC[ACT]^C
469 GAGCAC
BpuAI 639 GTCTTC GAAGAC(2/6)
1482 GTCTTC
BsaJI 1330 CCAAGG C^C[ACGT][ACGT]GG
1783 CCATGG
Bsp1286I 114 GGGCAC G[AGT]GC[ACT]^C
469 GAGCAC
BsrDI 283 GCAATG GCAATG(2/0)
1257 GCAATG
BsrI 1836 ACTGG ACTGG(1/-1)
630 CCAGT
BssKI 1076 CCTGG ^CC[ACGT]GG
1665 CCTGG
BstNI 1076 CCTGG CC^[AT]GG
1665 CCTGG
Cac8I 322 GCTTGC GC[ACGT]^[ACGT]GC
1635 GCGAGC
EcoRII 1076 CCTGG ^CC[AT]GG
1665 CCTGG
EcoT14I 1330 CCAAGG C^C[AT][AT]GG
1783 CCATGG
HaeIII 171 GGCC GG^CC
1356 GGCC
HapII 1448 CCGG C^CGG
1677 CCGG
HpaII 1448 CCGG C^CGG
1677 CCGG
HphI 1402 GGTGA GGTGA(8/7)
1649 TCACC
MaeI 604 CTAG C^TAG
1370 CTAG
MaeII 354 ACGT A^CGT
1707 ACGT
MspI 1448 CCGG C^CGG
1677 CCGG
MvaI 1076 CCTGG CC^[AT]GG
1665 CCTGG
MwoI 1130 GCTGTGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1384 GCTTCAGCTGC
ScrFI 1076 CCTGG CC^[ACGT]GG
1665 CCTGG
StyI 1330 CCAAGG C^C[AT][AT]GG
1783 CCATGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AvaII BbvI BsmAI BsmFI BstF5I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HinfI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MslI
MspA1I NdeII NlaIII PleI PvuII
RsaI Sau3AI Sau96I SfaNI SfcI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I