Restriction map for
pF1KA1464
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1593 GAATTT [GA]^AATT[CT]
Aor51HI 944 AGCGCT AGC^GCT
ApoI 1593 GAATTT [GA]^AATT[CT]
BcgI 1169 GCAATGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 126 GTATCC GTATCC(6/5)
BfaI 1399 CTAG C^TAG
BlpI 102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
BsgI 1217 CTGCAC GTGCAG(16/14)
BsmBI 497 GAGACG CGTCTC(1/5)
BsmI 441 GCATTC GAATGC(1/-1)
BsrDI 1169 GCAATG GCAATG(2/0)
BssSI 793 CTCGTG CACGAG(-5/-1)
BstAPI 759 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 421 GGTTACC G^GT[ACGT]ACC
BstPI 421 GGTTACC G^GT[ACGT]ACC
BstYI 890 AGATCC [GA]^GATC[CT]
CelII 102 GCTGAGC GC^T[ACGT]AGC
DraII 1445 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 944 AGCGCT AGC^GCT
EcoNI 1773 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1445 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 421 GGTTACC G^GT[ACGT]ACC
KspI 131 CCGCGG CCGC^GG
MaeI 1399 CTAG C^TAG
MaeII 500 ACGT A^CGT
MflI 890 AGATCC [GA]^GATC[CT]
MseI 1424 TTAA T^TAA
NcoI 426 CCATGG C^CATGG
PflMI 894 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1445 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1161 CAGCTG CAG^CTG
SacI 1053 GAGCTC GAGCT^C
SacII 131 CCGCGG CCGC^GG
SmaI 21 CCCGGG CCC^GGG
TfiI 852 GATTC G^A[AT]TC
Tru9I 1424 TTAA T^TAA
Van91I 894 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 426 CCATGGTGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 890 AGATCC [GA]^GATC[CT]
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 1331 GTAC GT^AC
1584 GTAC
AlwI 891 GATCC GGATC(4/5)
1529 GGATC
BanI 532 GGCACC G^G[CT][GA]CC
1429 GGTGCC
BanII 171 GAGCCC G[GA]GC[CT]^C
1053 GAGCTC
BglI 914 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1661 GCCCAGTTGGC
BsrFI 107 GCCGGC [GA]^CCGG[CT]
1001 GCCGGC
BstXI 468 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
558 CCACAGCCTTGG
Cfr10I 107 GCCGGC [GA]^CCGG[CT]
1001 GCCGGC
DrdI 1033 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1455 GACGGATGAGTC
NaeI 107 GCCGGC GCC^GGC
1001 GCCGGC
NgoMIV 107 GCCGGC G^CCGGC
1001 GCCGGC
RsaI 1331 GTAC GT^AC
1584 GTAC
SmlI 371 CTCAAG C^T[CT][GA]AG
994 CTCAAG
StuI 612 AGGCCT AGG^CCT
1815 AGGCCT
TaqI 834 TCGA T^CGA
932 TCGA
Tsp509I 1279 AATT ^AATT
1594 AATT
TthHB8I 834 TCGA T^CGA
932 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI