Restriction map for
pF1KA1124
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# NO CUT ENZYMES ( 73 enzymes) #
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Acc65I AccI AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AvrII BamHI BbeI BciVI
BclI BfrI BglII BlnI BsiEI
BsiWI BsmBI BsmI BspDI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FbaI
FseI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AatII 4453 GACGTC GACGT^C
AhdI 787 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 787 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 610 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 2644 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspHI 878 TCATGA T^CATGA
BspLU11I 2615 ACATGT A^CATGT
BstXI 4871 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 787 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4746 GATATC GAT^ATC
NcoI 4472 CCATGG C^CATGG
NdeI 3350 CATATG CA^TATG
PflFI 610 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3632 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 878 TCATGA T^CATGA
SexAI 4220 ACCTGGT A^CC[AT]GGT
SmaI 2492 CCCGGG CCC^GGG
SspI 1143 AATATT AAT^ATT
Tth111I 610 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3624 TCTAGA T^CTAGA
XmaCI 2492 CCCGGG C^CCGGG
XmaI 2492 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccIII 516 TCCGGA T^CCGGA
3966 TCCGGA
AflIII 2615 ACATGT A^C[GA][CT]GT
3191 ACGTGT
Asp700I 2458 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4080 GAAGCCATTC
AviII 4466 TGCGCA TGC^GCA
4643 TGCGCA
BbrPI 3190 CACGTG CAC^GTG
4771 CACGTG
BcgI 2481 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
207 GCAGCTTCATCG
BsaBI 1693 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3873 GATCGTAATC
BsaWI 516 TCCGGA [AT]^CCGG[AT]
3966 TCCGGA
BseAI 516 TCCGGA T^CCGGA
3966 TCCGGA
Bsp1407I 110 TGTACA T^GTACA
4433 TGTACA
BspEI 516 TCCGGA T^CCGGA
3966 TCCGGA
BsrDI 3423 CATTGC GCAATG(2/0)
307 GCAATG
BsrGI 110 TGTACA T^GTACA
4433 TGTACA
BssHII 3345 GCGCGC G^CGCGC
4367 GCGCGC
BssSI 856 CTCGTG CACGAG(-5/-1)
4917 CTCGTG
BstEII 2497 GGTTACC G^GT[ACGT]ACC
3798 GGTCACC
BstPI 2497 GGTTACC G^GT[ACGT]ACC
3798 GGTCACC
DrdI 94 GACGTGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1398 GACCGTGCAGTC
EcoO65I 2497 GGTTACC G^GT[ACGT]ACC
3798 GGTCACC
FspI 4466 TGCGCA TGC^GCA
4643 TGCGCA
HaeII 67 AGCGCC [GA]GCGC^[CT]
1545 GGCGCT
MamI 1693 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3873 GATCGTAATC
MroI 516 TCCGGA T^CCGGA
3966 TCCGGA
MslI 3321 CACGGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4800 CATGCAGGTG
NaeI 977 GCCGGC GCC^GGC
5118 GCCGGC
NgoMIV 977 GCCGGC G^CCGGC
5118 GCCGGC
PmaCI 3190 CACGTG CAC^GTG
4771 CACGTG
PmlI 3190 CACGTG CAC^GTG
4771 CACGTG
SbfI 741 CCTGCAGG CCTGCA^GG
1719 CCTGCAGG
SphI 1014 GCATGC GCATG^C
4799 GCATGC
Sse8387I 741 CCTGCAGG CCTGCA^GG
1719 CCTGCAGG
SspBI 110 TGTACA T^GTACA
4433 TGTACA
XmnI 2458 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4080 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII