Restriction map for
pF1KA0919
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiWI BsmBI
BsmI BspEI BspHI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SbfI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I StuI
StyI SwaI Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 846 GGTACC G^GTACC
AseI 399 ATTAAT AT^TAAT
AsnI 399 ATTAAT AT^TAAT
Asp718I 846 GGTACC G^GTACC
AvaI 920 CCCGGG C^[CT]CG[GA]G
AvaII 444 GGACC G^G[AT]CC
BamHI 59 GGATCC G^GATCC
BglII 1539 AGATCT A^GATCT
BsaI 1289 GAGACC GGTCTC(1/5)
BsaWI 1891 ACCGGA [AT]^CCGG[AT]
BseRI 257 GAGGAG GAGGAG(10/8)
BsgI 473 CTGCAC GTGCAG(16/14)
BsiEI 180 CGACCG CG[GA][CT]^CG
BsmFI 443 GGGAC GGGAC(10/14)
BsoBI 920 CCCGGG C^[CT]CG[GA]G
BssSI 52 CACGAG CACGAG(-5/-1)
DraII 853 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 853 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 809 GAATTC G^AATTC
FauI 6 GCGGG CCCGC(4/6)
HincII 359 GTCGAC GT[CT]^[GA]AC
HindII 359 GTCGAC GT[CT]^[GA]AC
KpnI 846 GGTACC GGTAC^C
MaeII 141 ACGT A^CGT
MslI 1740 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 858 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 399 ATTAAT AT^TAAT
PstI 1624 CTGCAG CTGCA^G
SalI 359 GTCGAC G^TCGAC
SapI 343 GAAGAGC GCTCTTC(1/4)
SexAI 1922 ACCTGGT A^CC[AT]GGT
SmaI 920 CCCGGG CCC^GGG
SmlI 515 CTCAAG C^T[CT][GA]AG
SpeI 781 ACTAGT A^CTAGT
Tsp45I 286 GTGAC ^GT[GC]AC
Van91I 858 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 495 TCTAGA T^CTAGA
XmaCI 920 CCCGGG C^CCGGG
XmaI 920 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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ApaI 100 GGGCCC GGGCC^C
854 GGGCCC
Asp700I 374 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1537 GAAGATCTTC
AspHI 49 GAGCAC G[AT]GC[AT]^C
78 GAGCAC
BanII 100 GGGCCC G[GA]GC[CT]^C
854 GGGCCC
BbsI 823 GAAGAC GAAGAC(2/6)
410 GTCTTC
BciVI 291 GGATAC GTATCC(6/5)
167 GTATCC
BcnI 920 CCCGG CC^[GC]GG
921 CCGGG
BpuAI 823 GAAGAC GAAGAC(2/6)
410 GTCTTC
BsaBI 1540 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1674 GATTATTATC
BsiHKAI 49 GAGCAC G[AT]GC[AT]^C
78 GAGCAC
Bsp1407I 428 TGTACA T^GTACA
1391 TGTACA
BspDI 530 ATCGAT AT^CGAT
887 ATCGAT
BspMI 509 ACCTGC ACCTGC(4/8)
1324 GCAGGT
BsrBI 4 GAGCGG CCGCTC(-3/-3)
55 GAGCGG
BsrDI 1935 CATTGC GCAATG(2/0)
337 GCAATG
BsrGI 428 TGTACA T^GTACA
1391 TGTACA
BstYI 59 GGATCC [GA]^GATC[CT]
1539 AGATCT
ClaI 530 ATCGAT AT^CGAT
887 ATCGAT
EcoT22I 746 ATGCAT ATGCA^T
997 ATGCAT
HapII 921 CCGG C^CGG
1892 CCGG
HindIII 154 AAGCTT A^AGCTT
565 AAGCTT
HpaII 921 CCGG C^CGG
1892 CCGG
MaeIII 286 GTGAC ^GT[ACGT]AC
1375 GTAAC
MamI 1540 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1674 GATTATTATC
MflI 59 GGATCC [GA]^GATC[CT]
1539 AGATCT
MspI 921 CCGG C^CGG
1892 CCGG
NciI 920 CCCGG CC^[GC]GG
921 CCGGG
NsiI 746 ATGCAT ATGCA^T
997 ATGCAT
PleI 229 GACTC GAGTC(4/5)
1608 GACTC
PvuII 1814 CAGCTG CAG^CTG
1867 CAGCTG
ScaI 778 AGTACT AGT^ACT
1712 AGTACT
SspBI 428 TGTACA T^GTACA
1391 TGTACA
SspI 376 AATATT AAT^ATT
1648 AATATT
XhoII 59 GGATCC [GA]^GATC[CT]
1539 AGATCT
XmnI 374 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1537 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BanI BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I