################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI StyI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 846 GGTACC G^GTACC AseI 399 ATTAAT AT^TAAT AsnI 399 ATTAAT AT^TAAT Asp718I 846 GGTACC G^GTACC AvaI 920 CCCGGG C^[CT]CG[GA]G AvaII 444 GGACC G^G[AT]CC BamHI 59 GGATCC G^GATCC BglII 1539 AGATCT A^GATCT BsaI 1289 GAGACC GGTCTC(1/5) BsaWI 1891 ACCGGA [AT]^CCGG[AT] BseRI 257 GAGGAG GAGGAG(10/8) BsgI 473 CTGCAC GTGCAG(16/14) BsiEI 180 CGACCG CG[GA][CT]^CG BsmFI 443 GGGAC GGGAC(10/14) BsoBI 920 CCCGGG C^[CT]CG[GA]G BssSI 52 CACGAG CACGAG(-5/-1) DraII 853 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 853 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 809 GAATTC G^AATTC FauI 6 GCGGG CCCGC(4/6) HincII 359 GTCGAC GT[CT]^[GA]AC HindII 359 GTCGAC GT[CT]^[GA]AC KpnI 846 GGTACC GGTAC^C MaeII 141 ACGT A^CGT MslI 1740 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 858 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 399 ATTAAT AT^TAAT PstI 1624 CTGCAG CTGCA^G SalI 359 GTCGAC G^TCGAC SapI 343 GAAGAGC GCTCTTC(1/4) SexAI 1922 ACCTGGT A^CC[AT]GGT SmaI 920 CCCGGG CCC^GGG SmlI 515 CTCAAG C^T[CT][GA]AG SpeI 781 ACTAGT A^CTAGT Tsp45I 286 GTGAC ^GT[GC]AC Van91I 858 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 495 TCTAGA T^CTAGA XmaCI 920 CCCGGG C^CCGGG XmaI 920 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### ApaI 100 GGGCCC GGGCC^C 854 GGGCCC Asp700I 374 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1537 GAAGATCTTC AspHI 49 GAGCAC G[AT]GC[AT]^C 78 GAGCAC BanII 100 GGGCCC G[GA]GC[CT]^C 854 GGGCCC BbsI 823 GAAGAC GAAGAC(2/6) 410 GTCTTC BciVI 291 GGATAC GTATCC(6/5) 167 GTATCC BcnI 920 CCCGG CC^[GC]GG 921 CCGGG BpuAI 823 GAAGAC GAAGAC(2/6) 410 GTCTTC BsaBI 1540 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1674 GATTATTATC BsiHKAI 49 GAGCAC G[AT]GC[AT]^C 78 GAGCAC Bsp1407I 428 TGTACA T^GTACA 1391 TGTACA BspDI 530 ATCGAT AT^CGAT 887 ATCGAT BspMI 509 ACCTGC ACCTGC(4/8) 1324 GCAGGT BsrBI 4 GAGCGG CCGCTC(-3/-3) 55 GAGCGG BsrDI 1935 CATTGC GCAATG(2/0) 337 GCAATG BsrGI 428 TGTACA T^GTACA 1391 TGTACA BstYI 59 GGATCC [GA]^GATC[CT] 1539 AGATCT ClaI 530 ATCGAT AT^CGAT 887 ATCGAT EcoT22I 746 ATGCAT ATGCA^T 997 ATGCAT HapII 921 CCGG C^CGG 1892 CCGG HindIII 154 AAGCTT A^AGCTT 565 AAGCTT HpaII 921 CCGG C^CGG 1892 CCGG MaeIII 286 GTGAC ^GT[ACGT]AC 1375 GTAAC MamI 1540 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1674 GATTATTATC MflI 59 GGATCC [GA]^GATC[CT] 1539 AGATCT MspI 921 CCGG C^CGG 1892 CCGG NciI 920 CCCGG CC^[GC]GG 921 CCGGG NsiI 746 ATGCAT ATGCA^T 997 ATGCAT PleI 229 GACTC GAGTC(4/5) 1608 GACTC PvuII 1814 CAGCTG CAG^CTG 1867 CAGCTG ScaI 778 AGTACT AGT^ACT 1712 AGTACT SspBI 428 TGTACA T^GTACA 1391 TGTACA SspI 376 AATATT AAT^ATT 1648 AATATT XhoII 59 GGATCC [GA]^GATC[CT] 1539 AGATCT XmnI 374 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1537 GAAGATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI BanI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I