Restriction map for
pF1KA0447
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaWI BseAI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 835 GACGTC GACGT^C
AccII 218 CGCG CG^CG
AcyI 835 GACGTC G[GA]^CG[CT]C
Alw44I 636 GTGCAC G^TGCAC
ApaI 1160 GGGCCC GGGCC^C
ApaLI 636 GTGCAC G^TGCAC
Asp700I 861 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 907 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 818 CCCGGG C^[CT]CG[GA]G
BalI 800 TGGCCA TGG^CCA
BclI 551 TGATCA T^GATCA
BfaI 319 CTAG C^TAG
BsaHI 835 GACGTC G[GA]^CG[CT]C
BsaI 178 GAGACC GGTCTC(1/5)
BseRI 852 GAGGAG GAGGAG(10/8)
BsiWI 941 CGTACG C^GTACG
BsmBI 517 GAGACG CGTCTC(1/5)
BsoBI 818 CCCGGG C^[CT]CG[GA]G
BspMI 68 GCAGGT ACCTGC(4/8)
BsrBI 855 GAGCGG CCGCTC(-3/-3)
BstUI 218 CGCG CG^CG
BstXI 692 CCAACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 237 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 551 TGATCA T^GATCA
HgaI 980 GCGTC GACGC(5/10)
Hin1I 835 GACGTC G[GA]^CG[CT]C
HincII 595 GTCAAC GT[CT]^[GA]AC
HindII 595 GTCAAC GT[CT]^[GA]AC
MaeI 319 CTAG C^TAG
MluNI 800 TGGCCA TGG^CCA
MscI 800 TGGCCA TGG^CCA
MslI 103 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 218 CGCG CG^CG
PflFI 907 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 879 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 180 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 81 CAGCTG CAG^CTG
SmaI 818 CCCGGG CCC^GGG
SphI 314 GCATGC GCATG^C
TaqI 191 TCGA T^CGA
TfiI 567 GATTC G^A[AT]TC
Tth111I 907 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 191 TCGA T^CGA
Van91I 879 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 689 CCACCAACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 818 CCCGGG C^CCGGG
XmaI 818 CCCGGG C^CCGGG
XmnI 861 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaII 1089 GGTCC G^G[AT]CC
1197 GGACC
BlpI 735 GCTCAGC GC^T[ACGT]AGC
1017 GCTCAGC
Bpu1102I 735 GCTCAGC GC^T[ACGT]AGC
1017 GCTCAGC
BsgI 261 CTGCAC GTGCAG(16/14)
328 GTGCAG
BsiEI 677 CGGCCG CG[GA][CT]^CG
1058 CGGCCG
BstEII 100 GGTCACC G^GT[ACGT]ACC
1077 GGTGACC
BstPI 100 GGTCACC G^GT[ACGT]ACC
1077 GGTGACC
CelII 735 GCTCAGC GC^T[ACGT]AGC
1017 GCTCAGC
DsaI 182 CCACGG C^C[GA][CT]GG
344 CCACGG
EagI 677 CGGCCG C^GGCCG
1058 CGGCCG
EclXI 677 CGGCCG C^GGCCG
1058 CGGCCG
Eco52I 677 CGGCCG C^GGCCG
1058 CGGCCG
EcoO65I 100 GGTCACC G^GT[ACGT]ACC
1077 GGTGACC
NspI 314 GCATGC [GA]CATG^[CT]
999 ACATGC
PleI 193 GAGTC GAGTC(4/5)
911 GAGTC
PpuMI 1088 GGGTCCT [GA]G^G[AT]CC[CT]
1196 AGGACCC
SacI 213 GAGCTC GAGCT^C
528 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
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AciI AfaI AluI AlwNI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI