Restriction map for
pF1KA0348
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AviII
AvrII BciVI BfrI BglII BlnI
BsaBI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI MamI NheI NruI NsiI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I RsrII SalI SapI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspBI SwaI TfiI Tth111I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1462 GACGTC GACGT^C
Acc65I 3993 GGTACC G^GTACC
AccI 1465 GTCTAC GT^[AC][GT]AC
AflIII 176 ACGCGT A^C[GA][CT]GT
AgeI 3343 ACCGGT A^CCGGT
AocI 4412 CCTTAGG CC^T[ACGT]AGG
Asp718I 3993 GGTACC G^GTACC
BbrPI 499 CACGTG CAC^GTG
BcgI 2607 CGATGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2354 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2354 GCTCAGC GC^T[ACGT]AGC
BsaAI 499 CACGTG [CT]AC^GT[GA]
BsiWI 179 CGTACG C^GTACG
BsrBI 2083 GAGCGG CCGCTC(-3/-3)
BssHII 73 GCGCGC G^CGCGC
BstEII 564 GGTCACC G^GT[ACGT]ACC
BstPI 564 GGTCACC G^GT[ACGT]ACC
Bsu36I 4412 CCTTAGG CC^T[ACGT]AGG
CelII 2354 GCTCAGC GC^T[ACGT]AGC
Eco81I 4412 CCTTAGG CC^T[ACGT]AGG
EcoO65I 564 GGTCACC G^GT[ACGT]ACC
KpnI 3993 GGTACC GGTAC^C
KspI 4118 CCGCGG CCGC^GG
MfeI 2771 CAATTG C^AATTG
MluI 176 ACGCGT A^CGCGT
MunI 2771 CAATTG C^AATTG
NaeI 106 GCCGGC GCC^GGC
NcoI 3396 CCATGG C^CATGG
NdeI 2043 CATATG CA^TATG
NgoMIV 106 GCCGGC G^CCGGC
NotI 77 GCGGCCGC GC^GGCCGC
NspI 1496 GCATGC [GA]CATG^[CT]
PinAI 3343 ACCGGT A^CCGGT
PmaCI 499 CACGTG CAC^GTG
PmlI 499 CACGTG CAC^GTG
PvuI 2579 CGATCG CGAT^CG
SacII 4118 CCGCGG CCGC^GG
SbfI 4035 CCTGCAGG CCTGCA^GG
ScaI 1798 AGTACT AGT^ACT
SphI 1496 GCATGC GCATG^C
Sse8387I 4035 CCTGCAGG CCTGCA^GG
SspI 1783 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccIII 1589 TCCGGA T^CCGGA
1634 TCCGGA
ApaI 2655 GGGCCC GGGCC^C
3796 GGGCCC
Asp700I 471 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1825 GAACAGCTTC
BamHI 1535 GGATCC G^GATCC
1586 GGATCC
BseAI 1589 TCCGGA T^CCGGA
1634 TCCGGA
BspEI 1589 TCCGGA T^CCGGA
1634 TCCGGA
BspHI 2151 TCATGA T^CATGA
4398 TCATGA
BstAPI 858 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4038 GCAGGTCCTGC
DrdI 421 GACCTGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2488 GACACCAAAGTC
EagI 78 CGGCCG C^GGCCG
2060 CGGCCG
EclXI 78 CGGCCG C^GGCCG
2060 CGGCCG
Eco52I 78 CGGCCG C^GGCCG
2060 CGGCCG
MroI 1589 TCCGGA T^CCGGA
1634 TCCGGA
PaeR7I 1204 CTCGAG C^TCGAG
3842 CTCGAG
RcaI 2151 TCATGA T^CATGA
4398 TCATGA
StuI 605 AGGCCT AGG^CCT
825 AGGCCT
XbaI 2466 TCTAGA T^CTAGA
2478 TCTAGA
XhoI 1204 CTCGAG C^TCGAG
3842 CTCGAG
XmnI 471 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1825 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII