Restriction map for
pF1KA0015
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AlwI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KasI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MunI NarI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1090 GACGTC GACGT^C
AccIII 485 TCCGGA T^CCGGA
AcsI 280 GAATTC [GA]^AATT[CT]
AcyI 1090 GACGTC G[GA]^CG[CT]C
Alw44I 637 GTGCAC G^TGCAC
ApaLI 637 GTGCAC G^TGCAC
ApoI 280 GAATTC [GA]^AATT[CT]
BbvCI 46 GCTGAGG CCTCAGC(-5/-2)
BsaHI 1090 GACGTC G[GA]^CG[CT]C
BsaWI 485 TCCGGA [AT]^CCGG[AT]
BseAI 485 TCCGGA T^CCGGA
BsgI 799 CTGCAC GTGCAG(16/14)
BsmBI 969 CGTCTC CGTCTC(1/5)
BspEI 485 TCCGGA T^CCGGA
BsrDI 783 CATTGC GCAATG(2/0)
BssHII 901 GCGCGC G^CGCGC
BstAPI 1308 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 280 GAATTC G^AATTC
HaeII 1038 GGCGCT [GA]GCGC^[CT]
Hin1I 1090 GACGTC G[GA]^CG[CT]C
HincII 952 GTCAAC GT[CT]^[GA]AC
HindII 952 GTCAAC GT[CT]^[GA]AC
HphI 855 GGTGA GGTGA(8/7)
MroI 485 TCCGGA T^CCGGA
MseI 389 TTAA T^TAA
NaeI 409 GCCGGC GCC^GGC
NgoMIV 409 GCCGGC G^CCGGC
PflMI 366 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 950 GAGTC GAGTC(4/5)
PstI 751 CTGCAG CTGCA^G
PvuII 256 CAGCTG CAG^CTG
TfiI 820 GATTC G^A[AT]TC
Tru9I 389 TTAA T^TAA
Tsp509I 281 AATT ^AATT
Van91I 366 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccII 571 CGCG CG^CG
902 CGCG
AflIII 518 ACGTGT A^C[GA][CT]GT
1007 ACGTGT
AocI 679 CCTGAGG CC^T[ACGT]AGG
1236 CCTCAGG
AspHI 142 GTGCTC G[AT]GC[AT]^C
637 GTGCAC
BalI 35 TGGCCA TGG^CCA
182 TGGCCA
BanI 429 GGTGCC G^G[CT][GA]CC
760 GGCACC
BbrPI 517 CACGTG CAC^GTG
634 CACGTG
BbsI 991 GTCTTC GAAGAC(2/6)
1231 GTCTTC
BlpI 144 GCTCAGC GC^T[ACGT]AGC
175 GCTGAGC
Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC
175 GCTGAGC
BpuAI 991 GTCTTC GAAGAC(2/6)
1231 GTCTTC
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
637 GTGCAC
BspMI 1061 ACCTGC ACCTGC(4/8)
426 GCAGGT
BsrFI 409 GCCGGC [GA]^CCGG[CT]
512 ACCGGC
BsrI 38 CCAGT ACTGG(1/-1)
414 CCAGT
BstUI 571 CGCG CG^CG
902 CGCG
Bsu36I 679 CCTGAGG CC^T[ACGT]AGG
1236 CCTCAGG
CelII 144 GCTCAGC GC^T[ACGT]AGC
175 GCTGAGC
Cfr10I 409 GCCGGC [GA]^CCGG[CT]
512 ACCGGC
DsaI 121 CCATGG C^C[GA][CT]GG
185 CCATGG
Eco81I 679 CCTGAGG CC^T[ACGT]AGG
1236 CCTCAGG
EcoT14I 121 CCATGG C^C[AT][AT]GG
185 CCATGG
HinfI 820 GATTC G^A[ACGT]TC
950 GAGTC
MaeIII 349 GTGAC ^GT[ACGT]AC
596 GTCAC
MluNI 35 TGGCCA TGG^CCA
182 TGGCCA
MscI 35 TGGCCA TGG^CCA
182 TGGCCA
MslI 765 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1218 CACGGTCATG
MvnI 571 CGCG CG^CG
902 CGCG
NcoI 121 CCATGG C^CATGG
185 CCATGG
PmaCI 517 CACGTG CAC^GTG
634 CACGTG
PmlI 517 CACGTG CAC^GTG
634 CACGTG
SapI 21 GAAGAGC GCTCTTC(1/4)
543 GCTCTTC
SfcI 262 CTACAG C^T[GA][CT]AG
751 CTGCAG
StyI 121 CCATGG C^C[AT][AT]GG
185 CCATGG
Tsp45I 349 GTGAC ^GT[GC]AC
596 GTCAC
XcmI 185 CCATGGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1104 CCAGGAAGTTGTTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I MaeII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SfaNI SmaI
TseI TspRI XmaCI XmaI