Restriction map for
pF1KSDF0258
(Circular) (Six-base) MAPSORT of: pF1KSDF0258.seq Check: 338 from: 1 to: 5443
pF1KSDF0258 5443 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 1363 1363
Size: 5443
AccI GT'mk_AC
Cuts at: 2479 2479
Size: 5443
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5443
AclI AA'CG_TT
Cuts at: 5283 5283
Size: 5443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1291 1465 1548 2095 2209 3266 3698 4447
Size: 222 174 83 547 114 1057 432 749
Cuts at: 4447 4715 1069
Size: 268 1797
Fragments arranged by size:
1797 1057 749 547 432 268 222 174
114 83
AfeI AGC'GCT
Cuts at: 2212 2212
Size: 5443
AflII C'TTAA_G
Cuts at: 649 649
Size: 5443
AflIII A'CryG_T
Cuts at: 2793 3899 4876 2793
Size: 1106 977 3360
Fragments arranged by size:
3360 1106 977
AgeI A'CCGG_T
Cuts at: 3831 3831
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1779 1779
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 144 255 870 1177 2226 2746 4315 144
Size: 111 615 307 1049 520 1569 1272
Fragments arranged by size:
1569 1272 1049 615 520 307 111
ApaI G_GGCC'C
Cuts at: 323 1547 1854 323
Size: 1224 307 3912
Fragments arranged by size:
3912 1224 307
ApaLI G'TGCA_C
Cuts at: 4213 4213
Size: 5443
ApoI r'AATT_y
Cuts at: 349 2445 4796 5052 349
Size: 2096 2351 256 740
Fragments arranged by size:
2351 2096 740 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5443
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5443
AvaI C'yCGr_G
Cuts at: 315 1533 1636 1787 1834 2461 315
Size: 1218 103 151 47 627 3297
Fragments arranged by size:
3297 1218 627 151 103 47
BamHI G'GATC_C
Cuts at: 1568 2466 1568
Size: 898 4545
BanI G'GyrC_C
Cuts at: 1436 2457 2871 3119 3154 1436
Size: 1021 414 248 35 3725
Fragments arranged by size:
3725 1021 414 248 35
BanII G_rGCy'C
Cuts at: 167 323 404 439 658 730 1279 1547 1663
Size: 156 81 35 219 72 549 268 116
Cuts at: 1663 1787 1835 1854 2252 2362 2455 3485 167
Size: 124 48 19 398 110 93 1030 2125
Fragments arranged by size:
2125 1030 549 398 268 219 156 124
116 110 93 81 72 48 35 19
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5195
BbsI GAAGACnn'nnnn_
Cuts at: 1342 2431 1342
Size: 1089 4354
BbvCI CC'TCA_GC
Cuts at: 202 1088 2347 202
Size: 886 1259 3298
Fragments arranged by size:
3298 1259 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1960 1994 1960
Size: 34 5409
BciVI GTATCCnnnnn_n'
Cuts at: 915 3339 4102 5443 915
Size: 2424 763 1341 915
Fragments arranged by size:
2424 1341 915 763
BclI T'GATC_A
Cuts at: 1233 2961 1233
Size: 1728 3715
BglII A'GATC_T
Cuts at: 2956 4995 2956
Size: 2039 3404
BlpI GC'TnA_GC
Cuts at: 178 2553 178
Size: 2375 3068
Bme1580I G_kGCm'C
Cuts at: 323 947 1547 1854 3066 3159 4217 323
Size: 624 600 307 1212 93 1058 1549
Fragments arranged by size:
1549 1212 1058 624 600 307 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 2653 2734 3069 5215 1108
Size: 1545 81 335 2146 1336
Fragments arranged by size:
2146 1545 1336 335 81
BmtI G_CTAG'C
Cuts at: 430 430
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 1677 3808 780
Size: 897 2131 2415
Fragments arranged by size:
2415 2131 897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1647 1917 2032 2203 166
Size: 1481 270 115 171 3406
Fragments arranged by size:
3406 1481 270 171 115
Bpu10I CC'TnA_GC
Cuts at: 202 422 1088 1781 2347 202
Size: 220 666 693 566 3298
Fragments arranged by size:
3298 693 666 566 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2617 3496 3990 4288 2617
Size: 879 494 298 3772
Fragments arranged by size:
3772 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 285 1219 1702 5147 285
Size: 934 483 3445 581
Fragments arranged by size:
3445 934 581 483
BsaAI yAC'GTr
Cuts at: 942 1698 2433 2794 942
Size: 756 735 361 3591
Fragments arranged by size:
3591 756 735 361
BsaBI GATnn'nnATC
Cuts at: 6 2955 4994 6
Size: 2949 2039 455
Fragments arranged by size:
2949 2039 455
BsaHI Gr'CG_yC
Cuts at: 1360 2872 3120 3824 5320 1360
Size: 1512 248 704 1496 1483
Fragments arranged by size:
1512 1496 1483 704 248
BsaWI w'CCGG_w
Cuts at: 1674 2630 3151 3831 4105 4252 4663 1674
Size: 956 521 680 274 147 411 2454
Fragments arranged by size:
2454 956 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 868 898 1085 1115 1858 1888 538
Size: 30 300 30 187 30 743 30 4093
Fragments arranged by size:
4093 743 300 187 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 727 772 1054 1387 1390 1843 1863 1987
Size: 3 45 282 333 3 453 20 124
Cuts at: 1987 2179 2370 724
Size: 192 191 3797
Fragments arranged by size:
3797 453 333 282 192 191 124 45
20 3 3
BseYI C'CCAG_C
Cuts at: 322 403 1732 2866 3624 4203 4699 322
Size: 81 1329 1134 758 579 496 1066
Fragments arranged by size:
1329 1134 1066 758 579 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 1669
Size: 5443
BsiEI CG_ry'CG
Cuts at: 104 458 2983 3029 4239 104
Size: 354 2525 46 1210 1308
Fragments arranged by size:
2525 1308 1210 354 46
BsiHKAI G_wGCw'C
Cuts at: 167 658 2455 3233 3423 4217 167
Size: 491 1797 778 190 794 1393
Fragments arranged by size:
1797 1393 794 778 491 190
BsmI GAATG_Cn'
Cuts at: 790 790
Size: 5443
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2020
Size: 5443
Bsp1286I G_dGCh'C
Cuts at: 167 323 404 439 658 730 947 1279 1547
Size: 156 81 35 219 72 217 332 268
Cuts at: 1547 1663 1787 1835 1854 2252 2362 2455 3066
Size: 116 124 48 19 398 110 93 611
Cuts at: 3066 3159 3233 3423 3485 4217 167
Size: 93 74 190 62 732 1393
Fragments arranged by size:
1393 732 611 398 332 268 219 217
190 156 124 116 110 93 93 81
74 72 62 48 35 19
BspEI T'CCGG_A
Cuts at: 1674 4663 1674
Size: 2989 2454
BspHI T'CATG_A
Cuts at: 4619 4619
Size: 5443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 934 1361 1493 1899 2491 2705 3007 3388
Size: 464 427 132 406 592 214 302 381
Cuts at: 3388 470
Size: 2525
Fragments arranged by size:
2525 592 464 427 406 381 302 214
132
BsrBI CCG'CTC
Cuts at: 50 474 3733 3787 5273 5438 50
Size: 424 3259 54 1486 165 55
Fragments arranged by size:
3259 1486 424 165 55 54
BsrDI GCAATG_nn'
Cuts at: 683 3353 683
Size: 2670 2773
BsrFI r'CCGG_y
Cuts at: 956 1580 3439 3620 3831 956
Size: 624 1859 181 211 2568
Fragments arranged by size:
2568 1859 624 211 181
BssSI C'ACGA_G
Cuts at: 193 970 3712 4072 193
Size: 777 2742 360 1564
Fragments arranged by size:
2742 1564 777 360
BstAPI GCAn_nnn'nTGC
Cuts at: 255 975 2818 255
Size: 720 1843 2880
Fragments arranged by size:
2880 1843 720
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 330 774 330
Size: 444 4999
BstYI r'GATC_y
Cuts at: 1 1108 1568 1759 2466 2933 2956 3291 3537
Size: 1107 460 191 707 467 23 335 246
Cuts at: 3537 4551 4637 4649 4995 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1107 1014 707 467 460 449 346 335
246 191 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 494 494
Size: 5443
BtgI C'CryG_G
Cuts at: 125 233 2129 125
Size: 108 1896 3439
Fragments arranged by size:
3439 1896 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1491 3391 3572 3735 1491
Size: 1900 181 163 3199
Fragments arranged by size:
3199 1900 181 163
BtsI GCAGTG_nn'
Cuts at: 835 1015 1887 2684 835
Size: 180 872 797 3594
Fragments arranged by size:
3594 872 797 180
DraI TTT'AAA
Cuts at: 2440 2440
Size: 5443
DraIII CAC_nnn'GTG
Cuts at: 777 777
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 1909 3147 4007 1909
Size: 1238 860 3345
Fragments arranged by size:
3345 1238 860
EaeI y'GGCC_r
Cuts at: 230 455 1215 1343 1592 2132 3026 3200 3591
Size: 225 760 128 249 540 894 174 391
Cuts at: 3591 3618 4965 230
Size: 27 1347 708
Fragments arranged by size:
1347 894 760 708 540 391 249 225
174 128 27
EagI C'GGCC_G
Cuts at: 455 3026 455
Size: 2571 2872
EarI CTCTTCn'nnn_
Cuts at: 503 701 718 1246 2059 2347 3464 3674 503
Size: 198 17 528 813 288 1117 210 2272
Fragments arranged by size:
2272 1117 813 528 288 210 198 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3961 4107 5253 3649
Size: 312 146 1146 3839
Fragments arranged by size:
3839 1146 312 146
EcoICRI GAG'CTC
Cuts at: 165 656 2453 165
Size: 491 1797 3155
Fragments arranged by size:
3155 1797 491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1069 1291 1465 1548 1647 1917 2032 2095
Size: 903 222 174 83 99 270 115 63
Cuts at: 2095 2203 2209 3266 3698 4447 4715 166
Size: 108 6 1057 432 749 268 894
Fragments arranged by size:
1057 903 894 749 432 270 268 222
174 115 108 99 83 63 6
EcoO109I rG'GnC_Cy
Cuts at: 319 513 526 1101 1544 1850 2580 319
Size: 194 13 575 443 306 730 3182
Fragments arranged by size:
3182 730 575 443 306 194 13
EcoRI G'AATT_C
Cuts at: 2445 2445
Size: 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 532 2229 3460 532
Size: 1697 1231 2515
Fragments arranged by size:
2515 1697 1231
FspI TGC'GCA
Cuts at: 1576 3222 1576
Size: 1646 3797
HaeII r_GCGC'y
Cuts at: 1684 1842 2172 2214 2875 3123 4147 5126 1684
Size: 158 330 42 661 248 1024 979 2001
Fragments arranged by size:
2001 1024 979 661 330 248 158 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1085 1100 1117 1132 1218 1250 3283
Size: 32 755 15 17 15 86 32 2033
Cuts at: 3283 3313 3315 3345 298
Size: 30 2 30 2396
Fragments arranged by size:
2396 2033 755 86 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 2331 2480 2331
Size: 149 5294
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5195
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5443
MluI A'CGCG_T
Cuts at: 4876 4876
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 2402 4114 4298 1112
Size: 1290 1712 184 2257
Fragments arranged by size:
2257 1712 1290 184
MscI TGG'CCA
Cuts at: 1594 2134 3202 1594
Size: 540 1068 3835
Fragments arranged by size:
3835 1068 540
MslI CAynn'nnrTG
Cuts at: 940 3430 3557 940
Size: 2490 127 2826
Fragments arranged by size:
2826 2490 127
MspA1I CmG'CkG
Cuts at: 153 843 2552 2866 3226 4241 4486 153
Size: 690 1709 314 360 1015 245 1110
Fragments arranged by size:
1709 1110 1015 690 360 314 245
NaeI GCC'GGC
Cuts at: 958 3622 958
Size: 2664 2779
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5195
NcoI C'CATG_G
Cuts at: 125 125
Size: 5443
NgoMIV G'CCGG_C
Cuts at: 956 3620 956
Size: 2664 2779
NheI G'CTAG_C
Cuts at: 426 426
Size: 5443
NruI TCG'CGA
Cuts at: 3804 3804
Size: 5443
NspI r_CATG'y
Cuts at: 384 2494 2994 3903 384
Size: 2110 500 909 1924
Fragments arranged by size:
2110 1924 909 500
PasI CC'CwG_GG
Cuts at: 987 2244 987
Size: 1257 4186
PciI A'CATG_T
Cuts at: 3899 3899
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 144 1899 2411 144
Size: 1755 512 3176
Fragments arranged by size:
3176 1755 512
PfoI T'CCnGG_A
Cuts at: 185 185
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4582 4614 4582
Size: 32 5411
PpuMI rG'GwC_Cy
Cuts at: 526 1101 526
Size: 575 4868
PshAI GACnn'nnGTC
Cuts at: 1625 1625
Size: 5443
PspOMI G'GGCC_C
Cuts at: 319 1543 1850 319
Size: 1224 307 3912
Fragments arranged by size:
3912 1224 307
PstI C_TGCA'G
Cuts at: 251 260 571 931 1022 1358 1882 2488 3173
Size: 9 311 360 91 336 524 606 685
Cuts at: 3173 251
Size: 2521
Fragments arranged by size:
2521 685 606 524 360 336 311 91
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5443
PvuII CAG'CTG
Cuts at: 153 843 2866 3226 153
Size: 690 2023 360 2370
Fragments arranged by size:
2370 2023 690 360
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5443
SacI G_AGCT'C
Cuts at: 167 658 2455 167
Size: 491 1797 3155
Fragments arranged by size:
3155 1797 491
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5443
SanDI GG'GwC_CC
Cuts at: 526 526
Size: 5443
SapI GCTCTTCn'nnn_
Cuts at: 718 1246 3464 3674 718
Size: 528 2218 210 2487
Fragments arranged by size:
2487 2218 528 210
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5443
ScaI AGT'ACT
Cuts at: 4960 4960
Size: 5443
SexAI A'CCwGG_T
Cuts at: 773 773
Size: 5443
SfcI C'TryA_G
Cuts at: 33 247 256 567 927 1018 1354 1878 2395
Size: 214 9 311 360 91 336 524 517
Cuts at: 2395 2484 3169 4164 4355 33
Size: 89 685 995 191 1121
Fragments arranged by size:
1121 995 685 524 517 360 336 311
214 191 91 89 9
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5195
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5443
SmaI CCC'GGG
Cuts at: 1535 2463 1535
Size: 928 4515
SmlI C'TyrA_G
Cuts at: 649 2596 3511 4005 4267 649
Size: 1947 915 494 262 1825
Fragments arranged by size:
1947 1825 915 494 262
SnaBI TAC'GTA
Cuts at: 2433 2433
Size: 5443
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5443
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4943
StuI AGG'CCT
Cuts at: 763 763
Size: 5443
StyI C'CwwG_G
Cuts at: 125 887 947 1212 2382 2405 2575 2929 125
Size: 762 60 265 1170 23 170 354 2639
Fragments arranged by size:
2639 1170 762 354 265 170 60 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 146 1000 1177 1183 1568 3024 3157 3616 3827
Size: 854 177 6 385 1456 133 459 211
Cuts at: 3827 146
Size: 1762
Fragments arranged by size:
1762 1456 854 459 385 211 177 133
6
TatI w'GTAC_w
Cuts at: 545 4958 545
Size: 4413 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1420 1452 1858 1890 2134 2166 4960 4992 1420
Size: 32 406 32 244 32 2794 32 1871
Fragments arranged by size:
2794 1871 406 244 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3238 3238
Size: 5443
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 2936
Size: 5443
XmaI C'CCGG_G
Cuts at: 1533 2461 1533
Size: 928 4515
XmnI GAAnn'nnTTC
Cuts at: 2084 2084
Size: 5443
ZraI GAC'GTC
Cuts at: 1361 1361
Size: 5443
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AloI AscI AvrII BaeI BfrBI
BglI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NsiI PacI PmlI PsiI PspXI PsrI
SacII SfiI SgrAI SrfI SspI SwaI XhoI