Restriction map for
pF1KSDB0050
(Circular) (Six-base) MAPSORT of: pF1KSDB0050.seq Check: 8877 from: 1 to: 7007
pF1KSDB0050 7007 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2037 2037
Size: 7007
AccI GT'mk_AC
Cuts at: 2999 4045 2999
Size: 1046 5961
Acc65I G'GTAC_C
Cuts at: 2129 4023 2129
Size: 1894 5113
AclI AA'CG_TT
Cuts at: 6847 6847
Size: 7007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 2960 3112 3694 3777 4832 5264 6011 6279
Size: 2284 152 582 83 1055 432 747 268
Cuts at: 6279 676
Size: 1404
Fragments arranged by size:
2284 1404 1055 747 582 432 268 152
83
AflII C'TTAA_G
Cuts at: 1489 1489
Size: 7007
AflIII A'CryG_T
Cuts at: 518 1133 3062 3620 4359 5463 6440 518
Size: 615 1929 558 739 1104 977 1085
Fragments arranged by size:
1929 1104 1085 977 739 615 558
AgeI A'CCGG_T
Cuts at: 5395 5395
Size: 7007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1085 1771 1085
Size: 686 6321
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 652
Size: 32 6975
AlwNI CAG_nnn'CTG
Cuts at: 2092 3177 3302 4312 5879 2092
Size: 1085 125 1010 1567 3220
Fragments arranged by size:
3220 1567 1085 1010 125
ApaI G_GGCC'C
Cuts at: 167 2445 3417 3960 167
Size: 2278 972 543 3214
Fragments arranged by size:
3214 2278 972 543
ApaLI G'TGCA_C
Cuts at: 5777 5777
Size: 7007
ApoI r'AATT_y
Cuts at: 466 3583 3636 4011 6360 6616 466
Size: 3117 53 375 2349 256 857
Fragments arranged by size:
3117 2349 857 375 256 53
AscI GG'CGCG_CC
Cuts at: 170 170
Size: 7007
AseI AT'TA_AT
Cuts at: 20 20
Size: 7007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7007
AvaI C'yCGr_G
Cuts at: 211 262 3857 3971 4027 211
Size: 51 3595 114 56 3191
Fragments arranged by size:
3595 3191 114 56 51
AvrII C'CTAG_G
Cuts at: 2727 2727
Size: 7007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1935 1968 1935
Size: 33 6974
BamHI G'GATC_C
Cuts at: 4032 4032
Size: 7007
BanI G'GyrC_C
Cuts at: 133 220 319 564 1548 2049 2129 4023 4437
Size: 87 99 245 984 501 80 1894 414
Cuts at: 4437 4685 4720 133
Size: 248 35 2420
Fragments arranged by size:
2420 1894 984 501 414 248 245 99
87 80 35
BanII G_rGCy'C
Cuts at: 167 1472 1730 1860 2445 2984 3417 3823 3858
Size: 1305 258 130 585 539 433 406 35
Cuts at: 3858 3960 4021 5051 167
Size: 102 61 1030 2123
Fragments arranged by size:
2123 1305 1030 585 539 433 406 258
130 102 61 35
BbeI G_GCGC'C
Cuts at: 137 323 4441 4689 137
Size: 186 4118 248 2455
Fragments arranged by size:
4118 2455 248 186
BbsI GAAGACnn'nnnn_
Cuts at: 277 385 808 2805 3511 3623 3682 277
Size: 108 423 1997 706 112 59 3602
Fragments arranged by size:
3602 1997 706 423 112 108 59
BbvCI CC'TCA_GC
Cuts at: 1526 2249 1526
Size: 723 6284
BciVI GTATCCnnnnn_n'
Cuts at: 546 1652 1963 3676 4905 5666 7007 546
Size: 1106 311 1713 1229 761 1341 546
Fragments arranged by size:
1713 1341 1229 1106 761 546 311
BclI T'GATC_A
Cuts at: 3743 4527 3743
Size: 784 6223
BglI GCCn_nnn'nGGC
Cuts at: 1333 1333
Size: 7007
BglII A'GATC_T
Cuts at: 1454 1673 4522 6559 1454
Size: 219 2849 2037 1902
Fragments arranged by size:
2849 2037 1902 219
BlpI GC'TnA_GC
Cuts at: 484 767 958 1820 2159 2426 4119 484
Size: 283 191 862 339 267 1693 3372
Fragments arranged by size:
3372 1693 862 339 283 267 191
Bme1580I G_kGCm'C
Cuts at: 167 223 1271 2054 2445 3417 3960 4632 4725
Size: 56 1048 783 391 972 543 672 93
Cuts at: 4725 5781 167
Size: 1056 1393
Fragments arranged by size:
1393 1056 1048 972 783 672 543 391
93 56
BmrI ACTGGGnnnn_n'
Cuts at: 1342 2898 3655 4219 4300 4635 6779 1342
Size: 1556 757 564 81 335 2144 1570
Fragments arranged by size:
2144 1570 1556 757 564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1776 3750 3837 5374 1776
Size: 1974 87 1537 3409
Fragments arranged by size:
3409 1974 1537 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 748 922 1143 1636 2428 2710 2998 3400
Size: 438 174 221 493 792 282 288 402
Cuts at: 3400 3497 3745 310
Size: 97 248 3572
Fragments arranged by size:
3572 792 493 438 402 288 282 248
221 174 97
Bpu10I CC'TnA_GC
Cuts at: 1526 2249 3647 1526
Size: 723 1398 4886
Fragments arranged by size:
4886 1398 723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5062 5554 5852 4183
Size: 879 492 298 5338
Fragments arranged by size:
5338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 965 1684 1843 1885 6711 965
Size: 719 159 42 4826 1261
Fragments arranged by size:
4826 1261 719 159 42
BsaAI yAC'GTr
Cuts at: 308 1136 2661 3099 3312 3999 4360 308
Size: 828 1525 438 213 687 361 2955
Fragments arranged by size:
2955 1525 828 687 438 361 213
BsaBI GATnn'nnATC
Cuts at: 6 401 623 1504 2776 4521 6558 6
Size: 395 222 881 1272 1745 2037 455
Fragments arranged by size:
2037 1745 1272 881 455 395 222
BsaHI Gr'CG_yC
Cuts at: 134 182 320 1809 2830 2927 4438 4686 5388
Size: 48 138 1489 1021 97 1511 248 702
Cuts at: 5388 6884 134
Size: 1496 257
Fragments arranged by size:
1511 1496 1489 1021 702 257 248 138
97 48
BsaWI w'CCGG_w
Cuts at: 596 1852 4196 4717 5395 5669 5816 6227 596
Size: 1256 2344 521 678 274 147 411 1376
Fragments arranged by size:
2344 1376 1256 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1132 1141 1171 1102
Size: 30 9 30 6938
Fragments arranged by size:
6938 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1006 1110 1113 1426 1558 1702 1735 1738 2413
Size: 104 3 313 132 144 33 3 675
Cuts at: 2413 2890 1006
Size: 477 5123
Fragments arranged by size:
5123 675 477 313 144 132 104 33
3 3
BseYI C'CCAG_C
Cuts at: 414 844 1229 1385 1422 1972 3408 4432 5190
Size: 430 385 156 37 550 1436 1024 758
Cuts at: 5190 5767 6263 414
Size: 577 496 1158
Fragments arranged by size:
1436 1158 1024 758 577 550 496 430
385 156 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 964 1291 1741 2528 178
Size: 786 327 450 787 4657
Fragments arranged by size:
4657 787 786 450 327
BsiEI CG_ry'CG
Cuts at: 104 427 856 4549 4595 5803 104
Size: 323 429 3693 46 1208 1308
Fragments arranged by size:
3693 1308 1208 429 323 46
BsiHKAI G_wGCw'C
Cuts at: 2154 2165 2708 2984 4021 4799 4989 5781 2154
Size: 11 543 276 1037 778 190 792 3380
Fragments arranged by size:
3380 1037 792 778 543 276 190 11
BsmBI CGTCTCn'nnnn_
Cuts at: 286 286
Size: 7007
Bsp1286I G_dGCh'C
Cuts at: 167 223 1271 1472 1730 1860 2054 2154 2165
Size: 56 1048 201 258 130 194 100 11
Cuts at: 2165 2445 2708 2984 3417 3823 3858 3960 4021
Size: 280 263 276 433 406 35 102 61
Cuts at: 4021 4632 4725 4799 4989 5051 5781 167
Size: 611 93 74 190 62 730 1393
Fragments arranged by size:
1393 1048 730 611 433 406 280 276
263 258 201 194 190 130 102 100
93 74 62 61 56 35 11
BspEI T'CCGG_A
Cuts at: 596 6227 596
Size: 5631 1376
BspHI T'CATG_A
Cuts at: 227 3287 6183 227
Size: 3060 2896 1051
Fragments arranged by size:
3060 2896 1051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2037 4057 4271 4573 4954 2037
Size: 2020 214 302 381 4090
Fragments arranged by size:
4090 2020 381 302 214
BsrBI CCG'CTC
Cuts at: 50 267 714 852 3816 5299 5353 6837 7002
Size: 217 447 138 2964 1483 54 1484 165
Cuts at: 7002 50
Size: 55
Fragments arranged by size:
2964 1484 1483 447 217 165 138 55
54
BsrDI GCAATG_nn'
Cuts at: 438 556 1379 2265 4919 438
Size: 118 823 886 2654 2526
Fragments arranged by size:
2654 2526 886 823 118
BsrFI r'CCGG_y
Cuts at: 2760 2801 3005 5005 5186 5395 2760
Size: 41 204 2000 181 209 4372
Fragments arranged by size:
4372 2000 209 204 181 41
BsrGI T'GTAC_A
Cuts at: 788 788
Size: 7007
BssHII G'CGCG_C
Cuts at: 170 170
Size: 7007
BssSI C'ACGA_G
Cuts at: 1876 2113 2183 5278 5636 1876
Size: 237 70 3095 358 3247
Fragments arranged by size:
3247 3095 358 237 70
BstAPI GCAn_nnn'nTGC
Cuts at: 4384 4384
Size: 7007
BstBI TT'CG_AA
Cuts at: 110 5368 110
Size: 5258 1749
BstEII G'GTnAC_C
Cuts at: 3778 3778
Size: 7007
BstXI CCAn_nnnn'nTGG
Cuts at: 1449 2826 1449
Size: 1377 5630
BstYI r'GATC_y
Cuts at: 1 365 1115 1454 1499 1673 2900 3212 4032
Size: 364 750 339 45 174 1227 312 820
Cuts at: 4032 4499 4522 4857 5103 6115 6201 6213 6559
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6559 1
Size: 449
Fragments arranged by size:
1227 1012 820 750 467 449 364 346
339 335 312 246 174 86 45 23
12
Bsu36I CC'TnA_GG
Cuts at: 2786 3831 2786
Size: 1045 5962
BtgI C'CryG_G
Cuts at: 125 1263 1448 1779 2681 3372 125
Size: 1138 185 331 902 691 3760
Fragments arranged by size:
3760 1138 902 691 331 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1800 4957 5138 5301 1800
Size: 3157 181 163 3506
Fragments arranged by size:
3506 3157 181 163
BtsI GCAGTG_nn'
Cuts at: 1371 1539 1704 1944 3698 4250 1371
Size: 168 165 240 1754 552 4128
Fragments arranged by size:
4128 1754 552 240 168 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2642 2677 2642
Size: 35 6972
DraI TTT'AAA
Cuts at: 4006 4006
Size: 7007
DrdI GACnn_nn'nnGTC
Cuts at: 2005 4713 5571 2005
Size: 2708 858 3441
Fragments arranged by size:
3441 2708 858
EaeI y'GGCC_r
Cuts at: 424 853 3435 3544 3849 4592 4766 5157 5184
Size: 429 2582 109 305 743 174 391 27
Cuts at: 5184 6529 424
Size: 1345 902
Fragments arranged by size:
2582 1345 902 743 429 391 305 174
109 27
EagI C'GGCC_G
Cuts at: 424 853 4592 424
Size: 429 3739 2839
Fragments arranged by size:
3739 2839 429
EarI CTCTTCn'nnn_
Cuts at: 2660 2812 3020 3289 5030 5240 2660
Size: 152 208 269 1741 210 4427
Fragments arranged by size:
4427 1741 269 210 208 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 1941 5215 5525 5671 6817 358
Size: 1583 3274 310 146 1146 548
Fragments arranged by size:
3274 1583 1146 548 310 146
EcoICRI GAG'CTC
Cuts at: 2982 4019 2982
Size: 1037 5970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 676 748 922 1143 1636 2428 2710 2960
Size: 366 72 174 221 493 792 282 250
Cuts at: 2960 2998 3112 3400 3497 3694 3745 3777 4832
Size: 38 114 288 97 197 51 32 1055
Cuts at: 4832 5264 6011 6279 310
Size: 432 747 268 1038
Fragments arranged by size:
1055 1038 792 747 493 432 366 288
282 268 250 221 197 174 114 97
72 51 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2449 3646 2449
Size: 1197 5810
EcoO109I rG'GnC_Cy
Cuts at: 411 651 687 1620 1976 2192 2441 2442 3279
Size: 240 36 933 356 216 249 1 837
Cuts at: 3279 3413 3720 3956 3957 4146 411
Size: 134 307 236 1 189 3272
Fragments arranged by size:
3272 933 837 356 307 249 240 236
216 189 134 36 1 1
EcoRI G'AATT_C
Cuts at: 4011 4011
Size: 7007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2007 5026 2007
Size: 3019 3988
FspI TGC'GCA
Cuts at: 4788 4788
Size: 7007
HaeII r_GCGC'y
Cuts at: 137 323 797 1827 2573 4441 4689 5711 6690
Size: 186 474 1030 746 1868 248 1022 979
Cuts at: 6690 137
Size: 454
Fragments arranged by size:
1868 1030 1022 979 746 474 454 248
186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 657 1141 1173 1191 1223 3052 3084 3131
Size: 32 484 32 18 32 1829 32 47
Cuts at: 3131 3163 4849 4879 4881 4911 625
Size: 32 1686 30 2 30 2721
Fragments arranged by size:
2721 1829 1686 484 47 32 32 32
32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 678 2331 2676 4046 678
Size: 1653 345 1370 3639
Fragments arranged by size:
3639 1653 1370 345
KasI G'GCGC_C
Cuts at: 133 319 4437 4685 133
Size: 186 4118 248 2455
Fragments arranged by size:
4118 2455 248 186
KpnI G_GTAC'C
Cuts at: 2133 4027 2133
Size: 1894 5113
MluI A'CGCG_T
Cuts at: 6440 6440
Size: 7007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1202 3212 5678 5862 1202
Size: 2010 2466 184 2347
Fragments arranged by size:
2466 2347 2010 184
MscI TGG'CCA
Cuts at: 3546 4768 3546
Size: 1222 5785
MslI CAynn'nnrTG
Cuts at: 226 1763 2170 2270 2824 4996 5123 226
Size: 1537 407 100 554 2172 127 2110
Fragments arranged by size:
2172 2110 1537 554 407 127 100
MspA1I CmG'CkG
Cuts at: 740 771 1065 2425 2640 3374 3840 4118 4432
Size: 31 294 1360 215 734 466 278 314
Cuts at: 4432 4792 5805 6050 740
Size: 360 1013 245 1697
Fragments arranged by size:
1697 1360 1013 734 466 360 314 294
278 245 215 31
NaeI GCC'GGC
Cuts at: 2762 3007 5188 2762
Size: 245 2181 4581
Fragments arranged by size:
4581 2181 245
NarI GG'CG_CC
Cuts at: 134 320 4438 4686 134
Size: 186 4118 248 2455
Fragments arranged by size:
4118 2455 248 186
NcoI C'CATG_G
Cuts at: 125 1263 1779 125
Size: 1138 516 5353
Fragments arranged by size:
5353 1138 516
NdeI CA'TA_TG
Cuts at: 2229 2229
Size: 7007
NgoMIV G'CCGG_C
Cuts at: 2760 3005 5186 2760
Size: 245 2181 4581
Fragments arranged by size:
4581 2181 245
NspI r_CATG'y
Cuts at: 522 666 699 1327 2169 3066 3624 4060 4560
Size: 144 33 628 842 897 558 436 500
Cuts at: 4560 5467 522
Size: 907 2062
Fragments arranged by size:
2062 907 897 842 628 558 500 436
144 33
PasI CC'CwG_GG
Cuts at: 373 373
Size: 7007
PciI A'CATG_T
Cuts at: 518 3062 3620 5463 518
Size: 2544 558 1843 2062
Fragments arranged by size:
2544 2062 1843 558
PflMI CCAn_nnn'nTGG
Cuts at: 2586 3177 3433 2586
Size: 591 256 6160
Fragments arranged by size:
6160 591 256
PfoI T'CCnGG_A
Cuts at: 1007 3074 1007
Size: 2067 4940
PmeI GTTT'AAAC
Cuts at: 4006 4006
Size: 7007
PmlI CAC'GTG
Cuts at: 308 308
Size: 7007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1759 1791 6146 6178 1759
Size: 32 4355 32 2588
Fragments arranged by size:
4355 2588 32 32
PpuMI rG'GwC_Cy
Cuts at: 411 651 687 1620 1976 3720 411
Size: 240 36 933 356 1744 3698
Fragments arranged by size:
3698 1744 933 356 240 36
PshAI GACnn'nnGTC
Cuts at: 1808 3785 3973 1808
Size: 1977 188 4842
Fragments arranged by size:
4842 1977 188
PspOMI G'GGCC_C
Cuts at: 163 2441 3413 3956 163
Size: 2278 972 543 3214
Fragments arranged by size:
3214 2278 972 543
PspXI vC'TCGA_Gb
Cuts at: 211 211
Size: 7007
PstI C_TGCA'G
Cuts at: 581 1378 2885 3146 3320 4054 4739 581
Size: 797 1507 261 174 734 685 2849
Fragments arranged by size:
2849 1507 797 734 685 261 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7007
PvuII CAG'CTG
Cuts at: 771 1065 2425 2640 3840 4432 4792 771
Size: 294 1360 215 1200 592 360 2986
Fragments arranged by size:
2986 1360 1200 592 360 294 215
RsrII CG'GwC_CG
Cuts at: 5202 5202
Size: 7007
SacI G_AGCT'C
Cuts at: 2984 4021 2984
Size: 1037 5970
SacII CC_GC'GG
Cuts at: 3375 3375
Size: 7007
SalI G'TCGA_C
Cuts at: 4044 4044
Size: 7007
SanDI GG'GwC_CC
Cuts at: 687 1976 687
Size: 1289 5718
SapI GCTCTTCn'nnn_
Cuts at: 2812 5030 5240 2812
Size: 2218 210 4579
Fragments arranged by size:
4579 2218 210
SbfI CC_TGCA'GG
Cuts at: 4054 4054
Size: 7007
ScaI AGT'ACT
Cuts at: 834 1840 6524 834
Size: 1006 4684 1317
Fragments arranged by size:
4684 1317 1006
SfcI C'TryA_G
Cuts at: 33 577 1374 2881 3142 3316 3711 4050 4735
Size: 544 797 1507 261 174 395 339 685
Cuts at: 4735 5728 5919 33
Size: 993 191 1121
Fragments arranged by size:
1507 1121 993 797 685 544 395 339
261 191 174
SfoI GGC'GCC
Cuts at: 135 321 4439 4687 135
Size: 186 4118 248 2455
Fragments arranged by size:
4118 2455 248 186
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7007
SmaI CCC'GGG
Cuts at: 3859 4029 3859
Size: 170 6837
SmlI C'TyrA_G
Cuts at: 211 1489 4162 5077 5569 5831 211
Size: 1278 2673 915 492 262 1387
Fragments arranged by size:
2673 1387 1278 915 492 262
SnaBI TAC'GTA
Cuts at: 3999 3999
Size: 7007
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7007
SphI G_CATG'C
Cuts at: 1327 4060 4560 1327
Size: 2733 500 3774
Fragments arranged by size:
3774 2733 500
StuI AGG'CCT
Cuts at: 3343 3671 3343
Size: 328 6679
StyI C'CwwG_G
Cuts at: 125 647 1172 1263 1730 1779 2727 3083 3164
Size: 522 525 91 467 49 948 356 81
Cuts at: 3164 3767 4141 4495 125
Size: 603 374 354 2637
Fragments arranged by size:
2637 948 603 525 522 467 374 356
354 91 81 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 582 1494 2880 4590 4723 5182 5391 582
Size: 912 1386 1710 133 459 209 2198
Fragments arranged by size:
2198 1710 1386 912 459 209 133
TatI w'GTAC_w
Cuts at: 788 832 1565 1838 3131 6522 788
Size: 44 733 273 1293 3391 1273
Fragments arranged by size:
3391 1293 1273 733 273 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6524 6556 6524
Size: 32 6975
Tth111I GACn'n_nGTC
Cuts at: 4804 4804
Size: 7007
XbaI T'CTAG_A
Cuts at: 4038 4038
Size: 7007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3844 4502 3844
Size: 658 6349
XhoI C'TCGA_G
Cuts at: 211 211
Size: 7007
XmaI C'CCGG_G
Cuts at: 3857 4027 3857
Size: 170 6837
XmnI GAAnn'nnTTC
Cuts at: 274 1774 274
Size: 1500 5507
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PsiI PsrI SexAI SfiI SgrAI SrfI SspI SwaI
ZraI