Restriction map for
pF1KSDA1195
(Circular) (Six-base) MAPSORT of: pF1KSDA1195.seq Check: 9064 from: 1 to: 5266
pF1KSDA1195 5266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 251 372 386 251
Size: 121 14 5131
Fragments arranged by size:
5131 121 14
AccI GT'mk_AC
Cuts at: 2302 2302
Size: 5266
Acc65I G'GTAC_C
Cuts at: 666 2280 666
Size: 1614 3652
AclI AA'CG_TT
Cuts at: 5106 5106
Size: 5266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1250 1557 2137 3089 3521 4270 4538 522
Size: 728 307 580 952 432 749 268 1250
Fragments arranged by size:
1250 952 749 728 580 432 307 268
AflIII A'CryG_T
Cuts at: 266 1049 2616 3722 4699 266
Size: 783 1567 1106 977 833
Fragments arranged by size:
1567 1106 977 833 783
AgeI A'CCGG_T
Cuts at: 3654 3654
Size: 5266
AhdI GACnn_n'nnGTC
Cuts at: 1623 1623
Size: 5266
AlwNI CAG_nnn'CTG
Cuts at: 811 2569 4138 811
Size: 1758 1569 1939
Fragments arranged by size:
1939 1758 1569
ApaLI G'TGCA_C
Cuts at: 1060 4036 1060
Size: 2976 2290
ApoI r'AATT_y
Cuts at: 822 1567 1836 2268 4619 4875 822
Size: 745 269 432 2351 256 1213
Fragments arranged by size:
2351 1213 745 432 269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
AvaI C'yCGr_G
Cuts at: 2198 2284 2198
Size: 86 5180
BamHI G'GATC_C
Cuts at: 2289 2289
Size: 5266
BanI G'GyrC_C
Cuts at: 155 253 666 2014 2280 2694 2942 2977 155
Size: 98 413 1348 266 414 248 35 2444
Fragments arranged by size:
2444 1348 414 413 266 248 98 35
BanII G_rGCy'C
Cuts at: 1209 1502 1745 2278 3308 1209
Size: 293 243 533 1030 3167
Fragments arranged by size:
3167 1030 533 293 243
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 974 940
Size: 34 5232
BciVI GTATCCnnnnn_n'
Cuts at: 1254 3162 3925 5266 1254
Size: 1908 763 1341 1254
Fragments arranged by size:
1908 1341 1254 763
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5266
BglII A'GATC_T
Cuts at: 1305 2039 2779 4818 1305
Size: 734 740 2039 1753
Fragments arranged by size:
2039 1753 740 734
BlpI GC'TnA_GC
Cuts at: 1783 2376 1783
Size: 593 4673
Bme1580I G_kGCm'C
Cuts at: 158 391 1064 2889 2982 4040 158
Size: 233 673 1825 93 1058 1384
Fragments arranged by size:
1825 1384 1058 673 233 93
BmgBI CAC'GTC
Cuts at: 1359 1359
Size: 5266
BmrI ACTGGGnnnn_n'
Cuts at: 479 2476 2557 2892 5038 479
Size: 1997 81 335 2146 707
Fragments arranged by size:
2146 1997 707 335 81
BmtI G_CTAG'C
Cuts at: 736 736
Size: 5266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3631 3631
Size: 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 1309 1760 1879 2043 1162
Size: 147 451 119 164 4385
Fragments arranged by size:
4385 451 164 147 119
Bpu10I CC'TnA_GC
Cuts at: 1148 1148
Size: 5266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2440 3319 3813 4111 1729
Size: 711 879 494 298 2884
Fragments arranged by size:
2884 879 711 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 472 4970 472
Size: 4498 768
BsaAI yAC'GTr
Cuts at: 1494 2256 2617 1494
Size: 762 361 4143
Fragments arranged by size:
4143 762 361
BsaBI GATnn'nnATC
Cuts at: 6 2778 4817 6
Size: 2772 2039 455
Fragments arranged by size:
2772 2039 455
BsaHI Gr'CG_yC
Cuts at: 2695 2943 3647 5143 2695
Size: 248 704 1496 2818
Fragments arranged by size:
2818 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2453 2974 3654 3928 4075 4486 2453
Size: 521 680 274 147 411 3233
Fragments arranged by size:
3233 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 626 656 626
Size: 30 5236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 505 604 650 283
Size: 222 99 46 4899
Fragments arranged by size:
4899 222 99 46
BseYI C'CCAG_C
Cuts at: 1879 1907 2078 2689 3447 4026 4522 1879
Size: 28 171 611 758 579 496 2623
Fragments arranged by size:
2623 758 611 579 496 171 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 1579
Size: 5266
BsiEI CG_ry'CG
Cuts at: 104 2806 2852 4062 104
Size: 2702 46 1210 1308
Fragments arranged by size:
2702 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 287 803 1064 1209 1502 1745 2114 2147 2278
Size: 516 261 145 293 243 369 33 131
Cuts at: 2278 3056 3246 4040 287
Size: 778 190 794 1513
Fragments arranged by size:
1513 794 778 516 369 293 261 243
190 145 131 33
BsmBI CGTCTCn'nnnn_
Cuts at: 2253 2253
Size: 5266
Bsp1286I G_dGCh'C
Cuts at: 158 287 391 803 1064 1209 1502 1745 2114
Size: 129 104 412 261 145 293 243 369
Cuts at: 2114 2147 2278 2889 2982 3056 3246 3308 4040
Size: 33 131 611 93 74 190 62 732
Cuts at: 4040 158
Size: 1384
Fragments arranged by size:
1384 732 611 412 369 293 261 243
190 145 131 129 104 93 74 62
33
BspEI T'CCGG_A
Cuts at: 4486 4486
Size: 5266
BspHI T'CATG_A
Cuts at: 459 470 4442 459
Size: 11 3972 1283
Fragments arranged by size:
3972 1283 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 300 372 386 1301 2314 2528 2830 3211
Size: 49 72 14 915 1013 214 302 381
Cuts at: 3211 251
Size: 2306
Fragments arranged by size:
2306 1013 915 381 302 214 72 49
14
BsrBI CCG'CTC
Cuts at: 50 3556 3610 5096 5261 50
Size: 3506 54 1486 165 55
Fragments arranged by size:
3506 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 1990 3176 1874
Size: 116 1186 3964
Fragments arranged by size:
3964 1186 116
BsrFI r'CCGG_y
Cuts at: 3262 3443 3654 3262
Size: 181 211 4874
Fragments arranged by size:
4874 211 181
BsrGI T'GTAC_A
Cuts at: 1105 1678 1105
Size: 573 4693
BssSI C'ACGA_G
Cuts at: 2234 3535 3895 2234
Size: 1301 360 3605
Fragments arranged by size:
3605 1301 360
BstAPI GCAn_nnn'nTGC
Cuts at: 811 2641 811
Size: 1830 3436
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5266
BstYI r'GATC_y
Cuts at: 1 142 541 1305 2039 2289 2756 2779 3114
Size: 141 399 764 734 250 467 23 335
Cuts at: 3114 3360 4374 4460 4472 4818 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 764 734 467 449 399 346 335
250 246 141 86 23 12
BtgI C'CryG_G
Cuts at: 125 413 968 2007 125
Size: 288 555 1039 3384
Fragments arranged by size:
3384 1039 555 288
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3214 3395 3558 3214
Size: 181 163 4922
Fragments arranged by size:
4922 181 163
BtsI GCAGTG_nn'
Cuts at: 189 647 1262 2507 189
Size: 458 615 1245 2948
Fragments arranged by size:
2948 1245 615 458
ClaI AT'CG_AT
Cuts at: 1653 1653
Size: 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 148 183 966 1001 148
Size: 35 783 35 4413
Fragments arranged by size:
4413 783 35 35
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 2970 3830 2970
Size: 860 4406
EaeI y'GGCC_r
Cuts at: 368 430 771 885 2849 3023 3414 3441 4788
Size: 62 341 114 1964 174 391 27 1347
Cuts at: 4788 368
Size: 846
Fragments arranged by size:
1964 1347 846 391 341 174 114 62
27
EagI C'GGCC_G
Cuts at: 2849 2849
Size: 5266
EarI CTCTTCn'nnn_
Cuts at: 541 2065 3287 3497 541
Size: 1524 1222 210 2310
Fragments arranged by size:
2310 1524 1222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1086 3472 3784 3930 5076 1086
Size: 2386 312 146 1146 1276
Fragments arranged by size:
2386 1276 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1207 1500 1743 2276 1207
Size: 293 243 533 4197
Fragments arranged by size:
4197 533 293 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1162 1250 1309 1557 1760 1879 2043 2137
Size: 640 88 59 248 203 119 164 94
Cuts at: 2137 3089 3521 4270 4538 522
Size: 952 432 749 268 1250
Fragments arranged by size:
1250 952 749 640 432 268 248 203
164 119 94 88 59
EcoO109I rG'GnC_Cy
Cuts at: 531 1290 2403 531
Size: 759 1113 3394
Fragments arranged by size:
3394 1113 759
EcoRI G'AATT_C
Cuts at: 1836 2268 1836
Size: 432 4834
EcoRV GAT'ATC
Cuts at: 657 657
Size: 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 3283 552
Size: 2731 2535
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5266
HaeII r_GCGC'y
Cuts at: 914 2698 2946 3970 4949 914
Size: 1784 248 1024 979 1231
Fragments arranged by size:
1784 1231 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 641 673 1609 1641 1646 1678 1958 1990 3106
Size: 32 936 32 5 32 280 32 1116
Cuts at: 3106 3136 3138 3168 641
Size: 30 2 30 2739
Fragments arranged by size:
2739 1116 936 280 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 2303 2303
Size: 5266
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5018
KpnI G_GTAC'C
Cuts at: 670 2284 670
Size: 1614 3652
MluI A'CGCG_T
Cuts at: 4699 4699
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4121 3937
Size: 184 5082
MscI TGG'CCA
Cuts at: 370 432 773 887 3025 370
Size: 62 341 114 2138 2611
Fragments arranged by size:
2611 2138 341 114 62
MslI CAynn'nnrTG
Cuts at: 202 2101 3253 3380 202
Size: 1899 1152 127 2088
Fragments arranged by size:
2088 1899 1152 127
MspA1I CmG'CkG
Cuts at: 428 1027 1471 2375 2689 3049 4064 4309 428
Size: 599 444 904 314 360 1015 245 1385
Fragments arranged by size:
1385 1015 904 599 444 360 314 245
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 5266
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5018
NcoI C'CATG_G
Cuts at: 125 413 968 2007 125
Size: 288 555 1039 3384
Fragments arranged by size:
3384 1039 555 288
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 5266
NheI G'CTAG_C
Cuts at: 732 732
Size: 5266
NruI TCG'CGA
Cuts at: 3627 3627
Size: 5266
NspI r_CATG'y
Cuts at: 270 1053 2178 2317 2817 3726 270
Size: 783 1125 139 500 909 1810
Fragments arranged by size:
1810 1125 909 783 500 139
PasI CC'CwG_GG
Cuts at: 1480 1708 1480
Size: 228 5038
PciI A'CATG_T
Cuts at: 266 1049 3722 266
Size: 783 2673 1810
Fragments arranged by size:
2673 1810 783
PflMI CCAn_nnn'nTGG
Cuts at: 366 419 894 1812 366
Size: 53 475 918 3820
Fragments arranged by size:
3820 918 475 53
PfoI T'CCnGG_A
Cuts at: 2020 2020
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5266
PmlI CAC'GTG
Cuts at: 1494 1494
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4405 4437 4405
Size: 32 5234
PpuMI rG'GwC_Cy
Cuts at: 531 1290 531
Size: 759 4507
PstI C_TGCA'G
Cuts at: 383 521 2311 2996 383
Size: 138 1790 685 2653
Fragments arranged by size:
2653 1790 685 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5266
PvuII CAG'CTG
Cuts at: 1027 2689 3049 1027
Size: 1662 360 3244
Fragments arranged by size:
3244 1662 360
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5266
SacI G_AGCT'C
Cuts at: 1209 1502 1745 2278 1209
Size: 293 243 533 4197
Fragments arranged by size:
4197 533 293 243
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5266
SapI GCTCTTCn'nnn_
Cuts at: 3287 3497 3287
Size: 210 5056
SbfI CC_TGCA'GG
Cuts at: 383 521 2311 383
Size: 138 1790 3338
Fragments arranged by size:
3338 1790 138
ScaI AGT'ACT
Cuts at: 4783 4783
Size: 5266
SexAI A'CCwGG_T
Cuts at: 758 758
Size: 5266
SfcI C'TryA_G
Cuts at: 33 379 517 1731 2307 2992 3987 4178 33
Size: 346 138 1214 576 685 995 191 1121
Fragments arranged by size:
1214 1121 995 685 576 346 191 138
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5018
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
SmaI CCC'GGG
Cuts at: 2286 2286
Size: 5266
SmlI C'TyrA_G
Cuts at: 1744 2419 3334 3828 4090 1744
Size: 675 915 494 262 2920
Fragments arranged by size:
2920 915 675 494 262
SnaBI TAC'GTA
Cuts at: 2256 2256
Size: 5266
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5266
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4766
SspI AAT'ATT
Cuts at: 786 786
Size: 5266
StyI C'CwwG_G
Cuts at: 125 413 968 991 1112 2007 2398 2752 125
Size: 288 555 23 121 895 391 354 2639
Fragments arranged by size:
2639 895 555 391 354 288 121 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 634 1125 2847 2980 3439 3650 634
Size: 491 1722 133 459 211 2250
Fragments arranged by size:
2250 1722 491 459 211 133
TatI w'GTAC_w
Cuts at: 1105 1678 1871 4781 1105
Size: 573 193 2910 1590
Fragments arranged by size:
2910 1590 573 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 626 658 1478 1510 1929 1961 2104 2136 4783
Size: 32 820 32 419 32 143 32 2647
Cuts at: 4783 4815 626
Size: 32 1077
Fragments arranged by size:
2647 1077 820 419 143 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 760 1894 3061 760
Size: 1134 1167 2965
Fragments arranged by size:
2965 1167 1134
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 247 750 1854 2759 247
Size: 503 1104 905 2754
Fragments arranged by size:
2754 1104 905 503
XmaI C'CCGG_G
Cuts at: 2284 2284
Size: 5266
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BsiWI BsmI
BssHII BstEII BstXI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NotI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI StuI SwaI XhoI XmnI ZraI