Restriction map for
pF1KSDA1155
(Circular) (Six-base) MAPSORT of: pF1KSDA1155.seq Check: 9177 from: 1 to: 3508
pF1KSDA1155 3508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3508
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3508
AclI AA'CG_TT
Cuts at: 3348 3348
Size: 3508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1331 1763 2512 2780 449
Size: 882 432 749 268 1177
Fragments arranged by size:
1177 882 749 432 268
AflIII A'CryG_T
Cuts at: 227 858 1964 2941 227
Size: 631 1106 977 794
Fragments arranged by size:
1106 977 794 631
AgeI A'CCGG_T
Cuts at: 1896 1896
Size: 3508
AhdI GACnn_n'nnGTC
Cuts at: 406 406
Size: 3508
AlwNI CAG_nnn'CTG
Cuts at: 811 2380 811
Size: 1569 1939
ApaLI G'TGCA_C
Cuts at: 2278 2278
Size: 3508
ApoI r'AATT_y
Cuts at: 510 2861 3117 510
Size: 2351 256 901
Fragments arranged by size:
2351 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3508
AvaI C'yCGr_G
Cuts at: 526 526
Size: 3508
BamHI G'GATC_C
Cuts at: 133 531 133
Size: 398 3110
BanI G'GyrC_C
Cuts at: 522 936 1184 1219 522
Size: 414 248 35 2811
Fragments arranged by size:
2811 414 248 35
BanII G_rGCy'C
Cuts at: 520 1550 520
Size: 1030 2478
BbeI G_GCGC'C
Cuts at: 940 1188 940
Size: 248 3260
BbsI GAAGACnn'nnnn_
Cuts at: 335 335
Size: 3508
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2167 3508 1404
Size: 763 1341 1404
Fragments arranged by size:
1404 1341 763
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3508
BglII A'GATC_T
Cuts at: 1021 3060 1021
Size: 2039 1469
BlpI GC'TnA_GC
Cuts at: 618 618
Size: 3508
Bme1580I G_kGCm'C
Cuts at: 197 1131 1224 2282 197
Size: 934 93 1058 1423
Fragments arranged by size:
1423 1058 934 93
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3280 718
Size: 81 335 2146 946
Fragments arranged by size:
2146 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2055 2353 682
Size: 879 494 298 1837
Fragments arranged by size:
1837 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 350 3212 350
Size: 2862 646
BsaAI yAC'GTr
Cuts at: 498 859 498
Size: 361 3147
BsaBI GATnn'nnATC
Cuts at: 6 1020 3059 6
Size: 1014 2039 455
Fragments arranged by size:
2039 1014 455
BsaHI Gr'CG_yC
Cuts at: 937 1185 1889 3385 937
Size: 248 704 1496 1060
Fragments arranged by size:
1496 1060 704 248
BsaWI w'CCGG_w
Cuts at: 695 1216 1896 2170 2317 2728 695
Size: 521 680 274 147 411 1475
Fragments arranged by size:
1475 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 283 283
Size: 3508
BseYI C'CCAG_C
Cuts at: 931 1689 2268 2764 931
Size: 758 579 496 1675
Fragments arranged by size:
1675 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1048 1094 2304 104
Size: 944 46 1210 1308
Fragments arranged by size:
1308 1210 944 46
BsiHKAI G_wGCw'C
Cuts at: 520 1298 1488 2282 520
Size: 778 190 794 1746
Fragments arranged by size:
1746 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 520 1131 1224 1298 1488 1550 2282 197
Size: 323 611 93 74 190 62 732 1423
Fragments arranged by size:
1423 732 611 323 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2728 2728
Size: 3508
BspHI T'CATG_A
Cuts at: 420 2684 420
Size: 2264 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 556 770 1072 1453 368
Size: 188 214 302 381 2423
Fragments arranged by size:
2423 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3338 3503 50
Size: 1748 54 1486 165 55
Fragments arranged by size:
1748 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3508
BsrFI r'CCGG_y
Cuts at: 1504 1685 1896 1504
Size: 181 211 3116
Fragments arranged by size:
3116 211 181
BssSI C'ACGA_G
Cuts at: 353 1777 2137 353
Size: 1424 360 1724
Fragments arranged by size:
1724 1424 360
BstAPI GCAn_nnn'nTGC
Cuts at: 883 883
Size: 3508
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3508
BstYI r'GATC_y
Cuts at: 1 133 531 998 1021 1356 1602 2616 2702
Size: 132 398 467 23 335 246 1014 86
Cuts at: 2702 2714 3060 1
Size: 12 346 449
Fragments arranged by size:
1014 467 449 398 346 335 246 132
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 365 365
Size: 3508
BtgI C'CryG_G
Cuts at: 371 371
Size: 3508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3164
Fragments arranged by size:
3164 181 163
BtsI GCAGTG_nn'
Cuts at: 749 749
Size: 3508
DraI TTT'AAA
Cuts at: 505 505
Size: 3508
DraIII CAC_nnn'GTG
Cuts at: 160 160
Size: 3508
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2072 1212
Size: 860 2648
EaeI y'GGCC_r
Cuts at: 1091 1265 1656 1683 3030 1091
Size: 174 391 27 1347 1569
Fragments arranged by size:
1569 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1091 1091
Size: 3508
EarI CTCTTCn'nnn_
Cuts at: 239 1529 1739 239
Size: 1290 210 2008
Fragments arranged by size:
2008 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1714 2026 2172 3318 1714
Size: 312 146 1146 1904
Fragments arranged by size:
1904 1146 312 146
EcoICRI GAG'CTC
Cuts at: 518 518
Size: 3508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1331 1763 2512 2780 449
Size: 882 432 749 268 1177
Fragments arranged by size:
1177 882 749 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 364 364
Size: 3508
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3508
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1525 1525
Size: 3508
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3508
HaeII r_GCGC'y
Cuts at: 940 1188 2212 3191 940
Size: 248 1024 979 1257
Fragments arranged by size:
1257 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 392 424 1348 1378 1380 1410 392
Size: 32 924 30 2 30 2490
Fragments arranged by size:
2490 924 32 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3508
KasI G'GCGC_C
Cuts at: 936 1184 936
Size: 248 3260
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3508
MluI A'CGCG_T
Cuts at: 2941 2941
Size: 3508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2179 2363 2179
Size: 184 3324
MscI TGG'CCA
Cuts at: 1267 1267
Size: 3508
MslI CAynn'nnrTG
Cuts at: 232 1495 1622 232
Size: 1263 127 2118
Fragments arranged by size:
2118 1263 127
MspA1I CmG'CkG
Cuts at: 302 617 931 1291 2306 2551 302
Size: 315 314 360 1015 245 1259
Fragments arranged by size:
1259 1015 360 315 314 245
NaeI GCC'GGC
Cuts at: 1687 1687
Size: 3508
NarI GG'CG_CC
Cuts at: 937 1185 937
Size: 248 3260
NcoI C'CATG_G
Cuts at: 371 371
Size: 3508
NgoMIV G'CCGG_C
Cuts at: 1685 1685
Size: 3508
NruI TCG'CGA
Cuts at: 1869 1869
Size: 3508
NspI r_CATG'y
Cuts at: 231 559 1059 1968 231
Size: 328 500 909 1771
Fragments arranged by size:
1771 909 500 328
PciI A'CATG_T
Cuts at: 227 1964 227
Size: 1737 1771
PfoI T'CCnGG_A
Cuts at: 480 480
Size: 3508
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2679 2647
Size: 32 3476
PstI C_TGCA'G
Cuts at: 281 553 1238 281
Size: 272 685 2551
Fragments arranged by size:
2551 685 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3508
PvuII CAG'CTG
Cuts at: 931 1291 931
Size: 360 3148
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3508
SacI G_AGCT'C
Cuts at: 520 520
Size: 3508
SalI G'TCGA_C
Cuts at: 543 543
Size: 3508
SapI GCTCTTCn'nnn_
Cuts at: 1529 1739 1529
Size: 210 3298
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3508
ScaI AGT'ACT
Cuts at: 493 3025 493
Size: 2532 976
SfcI C'TryA_G
Cuts at: 33 277 549 1234 2229 2420 33
Size: 244 272 685 995 191 1121
Fragments arranged by size:
1121 995 685 272 244 191
SfoI GGC'GCC
Cuts at: 938 1186 938
Size: 248 3260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3508
SmaI CCC'GGG
Cuts at: 528 528
Size: 3508
SmlI C'TyrA_G
Cuts at: 661 1576 2070 2332 661
Size: 915 494 262 1837
Fragments arranged by size:
1837 915 494 262
SnaBI TAC'GTA
Cuts at: 498 498
Size: 3508
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3508
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3008
StyI C'CwwG_G
Cuts at: 371 467 640 994 371
Size: 96 173 354 2885
Fragments arranged by size:
2885 354 173 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1089 1222 1681 1892 1089
Size: 133 459 211 2705
Fragments arranged by size:
2705 459 211 133
TatI w'GTAC_w
Cuts at: 224 491 3023 224
Size: 267 2532 709
Fragments arranged by size:
2532 709 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3025 3057 3025
Size: 32 3476
Tth111I GACn'n_nGTC
Cuts at: 1303 1303
Size: 3508
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 1001 187
Size: 814 2694
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3508
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI