Restriction map for
pF1KSDA1051
(Circular) (Six-base) MAPSORT of: pF1KSDA1051.seq Check: 2163 from: 1 to: 4210
pF1KSDA1051 4210 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 638 1246 638
Size: 608 3602
Acc65I G'GTAC_C
Cuts at: 1034 1106 1122 1224 1034
Size: 72 16 102 4020
Fragments arranged by size:
4020 102 72 16
AclI AA'CG_TT
Cuts at: 4050 4050
Size: 4210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2465 3214 3482 2033
Size: 432 749 268 2761
Fragments arranged by size:
2761 749 432 268
AflIII A'CryG_T
Cuts at: 708 1560 2666 3643 708
Size: 852 1106 977 1275
Fragments arranged by size:
1275 1106 977 852
AgeI A'CCGG_T
Cuts at: 2598 2598
Size: 4210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 800 832 800
Size: 32 4178
AlwNI CAG_nnn'CTG
Cuts at: 1513 3082 1513
Size: 1569 2641
ApaI G_GGCC'C
Cuts at: 304 304
Size: 4210
ApaLI G'TGCA_C
Cuts at: 2980 2980
Size: 4210
ApoI r'AATT_y
Cuts at: 233 918 955 1212 3563 3819 233
Size: 685 37 257 2351 256 624
Fragments arranged by size:
2351 685 624 257 256 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4210
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4210
AvaI C'yCGr_G
Cuts at: 1228 1228
Size: 4210
AvrII C'CTAG_G
Cuts at: 402 402
Size: 4210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1090 1114 1123 1147 1090
Size: 24 9 24 4153
Fragments arranged by size:
4153 24 24 9
BamHI G'GATC_C
Cuts at: 1233 1233
Size: 4210
BanI G'GyrC_C
Cuts at: 284 1034 1106 1122 1224 1638 1886 1921 284
Size: 750 72 16 102 414 248 35 2573
Fragments arranged by size:
2573 750 414 248 102 72 35 16
BanII G_rGCy'C
Cuts at: 304 813 870 1222 2252 304
Size: 509 57 352 1030 2262
Fragments arranged by size:
2262 1030 509 352 57
BbeI G_GCGC'C
Cuts at: 288 1642 1890 288
Size: 1354 248 2608
Fragments arranged by size:
2608 1354 248
BbsI GAAGACnn'nnnn_
Cuts at: 932 932
Size: 4210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 202 203 236 169
Size: 33 1 33 4143
Fragments arranged by size:
4143 33 33 1
BciVI GTATCCnnnnn_n'
Cuts at: 562 908 970 2106 2869 4210 562
Size: 346 62 1136 763 1341 562
Fragments arranged by size:
1341 1136 763 562 346 62
BclI T'GATC_A
Cuts at: 207 1085 1728 207
Size: 878 643 2689
Fragments arranged by size:
2689 878 643
BglII A'GATC_T
Cuts at: 1723 3762 1723
Size: 2039 2171
BlpI GC'TnA_GC
Cuts at: 418 1320 418
Size: 902 3308
Bme1580I G_kGCm'C
Cuts at: 304 1833 1926 2984 304
Size: 1529 93 1058 1530
Fragments arranged by size:
1530 1529 1058 93
BmgBI CAC'GTC
Cuts at: 636 636
Size: 4210
BmrI ACTGGGnnnn_n'
Cuts at: 298 695 1012 1033 1420 1501 1836 3982 298
Size: 397 317 21 387 81 335 2146 526
Fragments arranged by size:
2146 526 397 387 335 317 81 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 605 704 805 115
Size: 490 99 101 3520
Fragments arranged by size:
3520 490 101 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 2263 2757 3055 1384
Size: 879 494 298 2539
Fragments arranged by size:
2539 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3914 3914
Size: 4210
BsaAI yAC'GTr
Cuts at: 1200 1561 1200
Size: 361 3849
BsaBI GATnn'nnATC
Cuts at: 6 1722 3761 6
Size: 1716 2039 455
Fragments arranged by size:
2039 1716 455
BsaHI Gr'CG_yC
Cuts at: 285 1639 1887 2591 4087 285
Size: 1354 248 704 1496 408
Fragments arranged by size:
1496 1354 704 408 248
BsaWI w'CCGG_w
Cuts at: 1119 1397 1918 2598 2872 3019 3430 1119
Size: 278 521 680 274 147 411 1899
Fragments arranged by size:
1899 680 521 411 278 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 366 396 506 536 800 830 366
Size: 30 110 30 264 30 3746
Fragments arranged by size:
3746 264 110 30 30 30
BseYI C'CCAG_C
Cuts at: 1633 2391 2970 3466 1633
Size: 758 579 496 2377
Fragments arranged by size:
2377 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3006 104
Size: 1646 46 1210 1308
Fragments arranged by size:
1646 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 424 451 635 690 870 1222 2000 2190 2984
Size: 27 184 55 180 352 778 190 794
Cuts at: 2984 424
Size: 1650
Fragments arranged by size:
1650 794 778 352 190 184 180 55
27
BsmBI CGTCTCn'nnnn_
Cuts at: 1043 1043
Size: 4210
Bsp1286I G_dGCh'C
Cuts at: 304 424 451 635 690 813 870 1222 1833
Size: 120 27 184 55 123 57 352 611
Cuts at: 1833 1926 2000 2190 2252 2984 304
Size: 93 74 190 62 732 1530
Fragments arranged by size:
1530 732 611 352 190 184 123 120
93 74 62 57 55 27
BspEI T'CCGG_A
Cuts at: 3430 3430
Size: 4210
BspHI T'CATG_A
Cuts at: 3386 3386
Size: 4210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1258 1472 1774 2155 1258
Size: 214 302 381 3313
Fragments arranged by size:
3313 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4040 4205 50
Size: 2450 54 1486 165 55
Fragments arranged by size:
2450 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2120 2120
Size: 4210
BsrFI r'CCGG_y
Cuts at: 2206 2387 2598 2206
Size: 181 211 3818
Fragments arranged by size:
3818 211 181
BsrGI T'GTAC_A
Cuts at: 614 614
Size: 4210
BssSI C'ACGA_G
Cuts at: 2479 2839 2479
Size: 360 3850
BstAPI GCAn_nnn'nTGC
Cuts at: 1585 1585
Size: 4210
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4210
BstYI r'GATC_y
Cuts at: 1 1233 1700 1723 2058 2304 3318 3404 3416
Size: 1232 467 23 335 246 1014 86 12
Cuts at: 3416 3762 1
Size: 346 449
Fragments arranged by size:
1232 1014 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 503 2158 2339 2502 503
Size: 1655 181 163 2211
Fragments arranged by size:
2211 1655 181 163
BtsI GCAGTG_nn'
Cuts at: 489 1451 489
Size: 962 3248
ClaI AT'CG_AT
Cuts at: 179 179
Size: 4210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 248 283 248
Size: 35 4175
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4210
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2774 1914
Size: 860 3350
EaeI y'GGCC_r
Cuts at: 256 1793 1967 2358 2385 3732 256
Size: 1537 174 391 27 1347 734
Fragments arranged by size:
1537 1347 734 391 174 27
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4210
EarI CTCTTCn'nnn_
Cuts at: 749 823 2231 2441 749
Size: 74 1408 210 2518
Fragments arranged by size:
2518 1408 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2728 2874 4020 2416
Size: 312 146 1146 2606
Fragments arranged by size:
2606 1146 312 146
EcoICRI GAG'CTC
Cuts at: 868 1220 868
Size: 352 3858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 605 704 805 2033 2465 3214 3482 115
Size: 490 99 101 1228 432 749 268 843
Fragments arranged by size:
1228 843 749 490 432 268 101 99
EcoO109I rG'GnC_Cy
Cuts at: 1347 1347
Size: 4210
EcoRI G'AATT_C
Cuts at: 233 955 1212 233
Size: 722 257 3231
Fragments arranged by size:
3231 722 257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4210
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4210
HaeII r_GCGC'y
Cuts at: 288 1642 1890 2914 3893 288
Size: 1354 248 1024 979 605
Fragments arranged by size:
1354 1024 979 605 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 346 366 378 398 446 478 489
Size: 32 153 20 12 20 48 32 11
Cuts at: 489 521 2050 2080 2082 2112 161
Size: 32 1529 30 2 30 2259
Fragments arranged by size:
2259 1529 153 48 32 32 32 30
30 20 20 12 11 2
HincII GTy'rAC
Cuts at: 336 660 1247 336
Size: 324 587 3299
Fragments arranged by size:
3299 587 324
KasI G'GCGC_C
Cuts at: 284 1638 1886 284
Size: 1354 248 2608
Fragments arranged by size:
2608 1354 248
KpnI G_GTAC'C
Cuts at: 1038 1110 1126 1228 1038
Size: 72 16 102 4020
Fragments arranged by size:
4020 102 72 16
MfeI C'AATT_G
Cuts at: 275 275
Size: 4210
MluI A'CGCG_T
Cuts at: 3643 3643
Size: 4210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 814 2881 3065 814
Size: 2067 184 1959
Fragments arranged by size:
2067 1959 184
MscI TGG'CCA
Cuts at: 258 1969 258
Size: 1711 2499
MslI CAynn'nnrTG
Cuts at: 215 673 2197 2324 215
Size: 458 1524 127 2101
Fragments arranged by size:
2101 1524 458 127
MspA1I CmG'CkG
Cuts at: 1319 1633 1993 3008 3253 1319
Size: 314 360 1015 245 2276
Fragments arranged by size:
2276 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4210
NarI GG'CG_CC
Cuts at: 285 1639 1887 285
Size: 1354 248 2608
Fragments arranged by size:
2608 1354 248
NdeI CA'TA_TG
Cuts at: 996 996
Size: 4210
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4210
NruI TCG'CGA
Cuts at: 2571 2571
Size: 4210
NspI r_CATG'y
Cuts at: 712 1261 1761 2670 712
Size: 549 500 909 2252
Fragments arranged by size:
2252 909 549 500
PciI A'CATG_T
Cuts at: 708 2666 708
Size: 1958 2252
PflMI CCAn_nnn'nTGG
Cuts at: 565 565
Size: 4210
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 800 832 3349 3381 800
Size: 32 2517 32 1629
Fragments arranged by size:
2517 1629 32 32
PspOMI G'GGCC_C
Cuts at: 300 300
Size: 4210
PstI C_TGCA'G
Cuts at: 1255 1940 1255
Size: 685 3525
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4210
PvuII CAG'CTG
Cuts at: 1633 1993 1633
Size: 360 3850
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4210
SacI G_AGCT'C
Cuts at: 870 1222 870
Size: 352 3858
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4210
SapI GCTCTTCn'nnn_
Cuts at: 749 2231 2441 749
Size: 1482 210 2518
Fragments arranged by size:
2518 1482 210
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4210
ScaI AGT'ACT
Cuts at: 3727 3727
Size: 4210
SfcI C'TryA_G
Cuts at: 33 1251 1936 2931 3122 33
Size: 1218 685 995 191 1121
Fragments arranged by size:
1218 1121 995 685 191
SfoI GGC'GCC
Cuts at: 286 1640 1888 286
Size: 1354 248 2608
Fragments arranged by size:
2608 1354 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4210
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4210
SmlI C'TyrA_G
Cuts at: 1363 2278 2772 3034 1363
Size: 915 494 262 2539
Fragments arranged by size:
2539 915 494 262
SnaBI TAC'GTA
Cuts at: 1200 1200
Size: 4210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4210
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3710
StyI C'CwwG_G
Cuts at: 402 1342 1696 402
Size: 940 354 2916
Fragments arranged by size:
2916 940 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1791 1924 2383 2594 1791
Size: 133 459 211 3407
Fragments arranged by size:
3407 459 211 133
TatI w'GTAC_w
Cuts at: 614 3725 614
Size: 3111 1099
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 414 446 506 538 1080 1112 3727 3759 414
Size: 32 60 32 542 32 2615 32 865
Fragments arranged by size:
2615 865 542 60 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 378 2005 378
Size: 1627 2583
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4210
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4210
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NruI NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
BbvCI BfrBI BglI BmtI Bpu10I BseRI BsgI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I BtgI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NheI
NotI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI