Restriction map for
pF1KSDA0992
(Circular) (Six-base) MAPSORT of: pF1KSDA0992.seq Check: 8211 from: 1 to: 5155
pF1KSDA0992 5155 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 795 1909 2191 795
Size: 1114 282 3759
Fragments arranged by size:
3759 1114 282
Acc65I G'GTAC_C
Cuts at: 1736 2169 1736
Size: 433 4722
AclI AA'CG_TT
Cuts at: 4995 4995
Size: 5155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 1374 2978 3410 4159 4427 396
Size: 978 1604 432 749 268 1124
Fragments arranged by size:
1604 1124 978 749 432 268
AfeI AGC'GCT
Cuts at: 558 558
Size: 5155
AflIII A'CryG_T
Cuts at: 443 1054 1612 2505 3611 4588 443
Size: 611 558 893 1106 977 1010
Fragments arranged by size:
1106 1010 977 893 611 558
AgeI A'CCGG_T
Cuts at: 3543 3543
Size: 5155
AlwNI CAG_nnn'CTG
Cuts at: 2458 4027 2458
Size: 1569 3586
ApaI G_GGCC'C
Cuts at: 227 227
Size: 5155
ApaLI G'TGCA_C
Cuts at: 1561 3925 1561
Size: 2364 2791
ApoI r'AATT_y
Cuts at: 680 2157 4508 4764 680
Size: 1477 2351 256 1071
Fragments arranged by size:
2351 1477 1071 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5155
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5155
AvaI C'yCGr_G
Cuts at: 220 355 477 483 2173 220
Size: 135 122 6 1690 3202
Fragments arranged by size:
3202 1690 135 122 6
BamHI G'GATC_C
Cuts at: 2178 2178
Size: 5155
BanI G'GyrC_C
Cuts at: 435 990 1736 2169 2583 2831 2866 435
Size: 555 746 433 414 248 35 2724
Fragments arranged by size:
2724 746 555 433 414 248 35
BanII G_rGCy'C
Cuts at: 227 356 373 484 2167 3197 227
Size: 129 17 111 1683 1030 2185
Fragments arranged by size:
2185 1683 1030 129 111 17
BbeI G_GCGC'C
Cuts at: 2587 2835 2587
Size: 248 4907
BbsI GAAGACnn'nnnn_
Cuts at: 402 402
Size: 5155
BbvCI CC'TCA_GC
Cuts at: 553 2022 553
Size: 1469 3686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2027 2061 2027
Size: 34 5121
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3814 5155 3051
Size: 763 1341 3051
Fragments arranged by size:
3051 1341 763
BclI T'GATC_A
Cuts at: 1128 1571 2673 1128
Size: 443 1102 3610
Fragments arranged by size:
3610 1102 443
BglII A'GATC_T
Cuts at: 1025 1085 1112 1149 1419 1485 2668 4707 1025
Size: 60 27 37 270 66 1183 2039 1473
Fragments arranged by size:
2039 1473 1183 270 66 60 37 27
BlpI GC'TnA_GC
Cuts at: 350 2265 350
Size: 1915 3240
Bme1580I G_kGCm'C
Cuts at: 227 440 1565 2778 2871 3929 227
Size: 213 1125 1213 93 1058 1453
Fragments arranged by size:
1453 1213 1125 1058 213 93
BmgBI CAC'GTC
Cuts at: 444 1589 444
Size: 1145 4010
BmrI ACTGGGnnnn_n'
Cuts at: 1735 1968 2365 2446 2781 4927 1735
Size: 233 397 81 335 2146 1963
Fragments arranged by size:
2146 1963 397 335 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 5155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 861 1436 1675 237
Size: 624 575 239 3717
Fragments arranged by size:
3717 624 575 239
Bpu10I CC'TnA_GC
Cuts at: 553 2022 553
Size: 1469 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 3208 3702 4000 2329
Size: 879 494 298 3484
Fragments arranged by size:
3484 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 932 1307 4859 932
Size: 375 3552 1228
Fragments arranged by size:
3552 1228 375
BsaAI yAC'GTr
Cuts at: 1594 2145 2506 1594
Size: 551 361 4243
Fragments arranged by size:
4243 551 361
BsaBI GATnn'nnATC
Cuts at: 6 2667 4706 6
Size: 2661 2039 455
Fragments arranged by size:
2661 2039 455
BsaHI Gr'CG_yC
Cuts at: 295 493 538 2584 2832 3536 5032 295
Size: 198 45 2046 248 704 1496 418
Fragments arranged by size:
2046 1496 704 418 248 198 45
BsaWI w'CCGG_w
Cuts at: 2342 2863 3543 3817 3964 4375 2342
Size: 521 680 274 147 411 3122
Fragments arranged by size:
3122 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 458 488 859 889 458
Size: 30 371 30 4724
Fragments arranged by size:
4724 371 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 363 366 363
Size: 3 5152
BseYI C'CCAG_C
Cuts at: 259 1900 2578 3336 3915 4411 259
Size: 1641 678 758 579 496 1003
Fragments arranged by size:
1641 1003 758 678 579 496
BsiEI CG_ry'CG
Cuts at: 104 312 590 2695 2741 3951 104
Size: 208 278 2105 46 1210 1308
Fragments arranged by size:
2105 1308 1210 278 208 46
BsiHKAI G_wGCw'C
Cuts at: 1533 1565 1999 2167 2945 3135 3929 1533
Size: 32 434 168 778 190 794 2759
Fragments arranged by size:
2759 794 778 434 190 168 32
BsiWI C'GTAC_G
Cuts at: 1515 1515
Size: 5155
BsmI GAATG_Cn'
Cuts at: 1043 1043
Size: 5155
BsmBI CGTCTCn'nnnn_
Cuts at: 1352 1352
Size: 5155
Bsp1286I G_dGCh'C
Cuts at: 227 356 373 440 484 1533 1565 1999 2167
Size: 129 17 67 44 1049 32 434 168
Cuts at: 2167 2778 2871 2945 3135 3197 3929 227
Size: 611 93 74 190 62 732 1453
Fragments arranged by size:
1453 1049 732 611 434 190 168 129
93 74 67 62 44 32 17
BspEI T'CCGG_A
Cuts at: 4375 4375
Size: 5155
BspHI T'CATG_A
Cuts at: 4331 4331
Size: 5155
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 2203 2417 2719 3100 1171
Size: 1032 214 302 381 3226
Fragments arranged by size:
3226 1032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 146 474 3445 3499 4985 5150 50
Size: 96 328 2971 54 1486 165 55
Fragments arranged by size:
2971 1486 328 165 96 55 54
BsrDI GCAATG_nn'
Cuts at: 3065 3065
Size: 5155
BsrFI r'CCGG_y
Cuts at: 246 582 1600 3151 3332 3543 246
Size: 336 1018 1551 181 211 1858
Fragments arranged by size:
1858 1551 1018 336 211 181
BssHII G'CGCG_C
Cuts at: 131 187 131
Size: 56 5099
BssSI C'ACGA_G
Cuts at: 3424 3784 3424
Size: 360 4795
BstAPI GCAn_nnn'nTGC
Cuts at: 2530 2530
Size: 5155
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5155
BstEII G'GTnAC_C
Cuts at: 1472 1472
Size: 5155
BstYI r'GATC_y
Cuts at: 1 1025 1085 1112 1149 1419 1485 2178 2645
Size: 1024 60 27 37 270 66 693 467
Cuts at: 2645 2668 3003 3249 4263 4349 4361 4707 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1024 1014 693 467 449 346 335 270
246 86 66 60 37 27 23 12
BstZ17I GTA'TAC
Cuts at: 1910 1910
Size: 5155
Bsu36I CC'TnA_GG
Cuts at: 1232 1308 2084 1232
Size: 76 776 4303
Fragments arranged by size:
4303 776 76
BtgI C'CryG_G
Cuts at: 237 364 419 528 542 1854 237
Size: 127 55 109 14 1312 3538
Fragments arranged by size:
3538 1312 127 109 55 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 189 3103 3284 3447 189
Size: 2914 181 163 1897
Fragments arranged by size:
2914 1897 181 163
BtsI GCAGTG_nn'
Cuts at: 451 498 2396 451
Size: 47 1898 3210
Fragments arranged by size:
3210 1898 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1449 1484 1449
Size: 35 5120
DraI TTT'AAA
Cuts at: 1098 2152 1098
Size: 1054 4101
DraIII CAC_nnn'GTG
Cuts at: 1976 1976
Size: 5155
DrdI GACnn_nn'nnGTC
Cuts at: 1462 2859 3719 1462
Size: 1397 860 2898
Fragments arranged by size:
2898 1397 860
EaeI y'GGCC_r
Cuts at: 309 525 531 587 756 2738 2912 3303 3330
Size: 216 6 56 169 1982 174 391 27
Cuts at: 3330 4677 309
Size: 1347 787
Fragments arranged by size:
1982 1347 787 391 216 174 169 56
27 6
EagI C'GGCC_G
Cuts at: 309 587 2738 309
Size: 278 2151 2726
Fragments arranged by size:
2726 2151 278
EarI CTCTTCn'nnn_
Cuts at: 839 3176 3386 839
Size: 2337 210 2608
Fragments arranged by size:
2608 2337 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 138 3361 3673 3819 4965 138
Size: 3223 312 146 1146 328
Fragments arranged by size:
3223 1146 328 312 146
EcoICRI GAG'CTC
Cuts at: 2165 2165
Size: 5155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 396 861 1374 1436 1675 2978 3410 4159
Size: 159 465 513 62 239 1303 432 749
Cuts at: 4159 4427 237
Size: 268 965
Fragments arranged by size:
1303 965 749 513 465 432 268 239
159 62
EcoO109I rG'GnC_Cy
Cuts at: 360 741 1160 2136 2292 360
Size: 381 419 976 156 3223
Fragments arranged by size:
3223 976 419 381 156
EcoRI G'AATT_C
Cuts at: 2157 2157
Size: 5155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 5155
FspI TGC'GCA
Cuts at: 2934 2934
Size: 5155
HaeII r_GCGC'y
Cuts at: 350 560 2587 2835 3859 4838 350
Size: 210 2027 248 1024 979 667
Fragments arranged by size:
2027 1024 979 667 248 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2048 2080 2995 3025 3027 3057 2048
Size: 32 915 30 2 30 4146
Fragments arranged by size:
4146 915 32 30 30 2
HincII GTy'rAC
Cuts at: 594 770 796 1278 2192 594
Size: 176 26 482 914 3557
Fragments arranged by size:
3557 914 482 176 26
HpaI GTT'AAC
Cuts at: 770 770
Size: 5155
KasI G'GCGC_C
Cuts at: 2583 2831 2583
Size: 248 4907
KpnI G_GTAC'C
Cuts at: 1740 2173 1740
Size: 433 4722
MluI A'CGCG_T
Cuts at: 4588 4588
Size: 5155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2103 3826 4010 2103
Size: 1723 184 3248
Fragments arranged by size:
3248 1723 184
MscI TGG'CCA
Cuts at: 758 2914 758
Size: 2156 2999
MslI CAynn'nnrTG
Cuts at: 1319 3142 3269 1319
Size: 1823 127 3205
Fragments arranged by size:
3205 1823 127
MspA1I CmG'CkG
Cuts at: 544 881 2264 2578 2938 3953 4198 544
Size: 337 1383 314 360 1015 245 1501
Fragments arranged by size:
1501 1383 1015 360 337 314 245
NaeI GCC'GGC
Cuts at: 248 584 1602 3334 248
Size: 336 1018 1732 2069
Fragments arranged by size:
2069 1732 1018 336
NarI GG'CG_CC
Cuts at: 2584 2832 2584
Size: 248 4907
NgoMIV G'CCGG_C
Cuts at: 246 582 1600 3332 246
Size: 336 1018 1732 2069
Fragments arranged by size:
2069 1732 1018 336
NruI TCG'CGA
Cuts at: 3516 3516
Size: 5155
NspI r_CATG'y
Cuts at: 1058 1616 1842 2206 2706 3615 1058
Size: 558 226 364 500 909 2598
Fragments arranged by size:
2598 909 558 500 364 226
PasI CC'CwG_GG
Cuts at: 600 600
Size: 5155
PciI A'CATG_T
Cuts at: 1054 1612 3611 1054
Size: 558 1999 2598
Fragments arranged by size:
2598 1999 558
PfoI T'CCnGG_A
Cuts at: 1413 1413
Size: 5155
PmeI GTTT'AAAC
Cuts at: 2152 2152
Size: 5155
PmlI CAC'GTG
Cuts at: 1594 1594
Size: 5155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 878 910 4294 4326 878
Size: 32 3384 32 1707
Fragments arranged by size:
3384 1707 32 32
PpuMI rG'GwC_Cy
Cuts at: 741 1160 2136 741
Size: 419 976 3760
Fragments arranged by size:
3760 976 419
PshAI GACnn'nnGTC
Cuts at: 492 492
Size: 5155
PspOMI G'GGCC_C
Cuts at: 223 223
Size: 5155
PstI C_TGCA'G
Cuts at: 1450 2200 2885 1450
Size: 750 685 3720
Fragments arranged by size:
3720 750 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5155
PvuII CAG'CTG
Cuts at: 881 2578 2938 881
Size: 1697 360 3098
Fragments arranged by size:
3098 1697 360
RsrII CG'GwC_CG
Cuts at: 3348 3348
Size: 5155
SacI G_AGCT'C
Cuts at: 2167 2167
Size: 5155
SacII CC_GC'GG
Cuts at: 545 545
Size: 5155
SalI G'TCGA_C
Cuts at: 794 2190 794
Size: 1396 3759
SapI GCTCTTCn'nnn_
Cuts at: 3176 3386 3176
Size: 210 4945
SbfI CC_TGCA'GG
Cuts at: 2200 2200
Size: 5155
ScaI AGT'ACT
Cuts at: 4672 4672
Size: 5155
SfcI C'TryA_G
Cuts at: 33 1446 2196 2881 3876 4067 33
Size: 1413 750 685 995 191 1121
Fragments arranged by size:
1413 1121 995 750 685 191
SfoI GGC'GCC
Cuts at: 2585 2833 2585
Size: 248 4907
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5155
SgrAI Cr'CCGG_yG
Cuts at: 582 582
Size: 5155
SmaI CCC'GGG
Cuts at: 485 2175 485
Size: 1690 3465
SmlI C'TyrA_G
Cuts at: 2308 3223 3717 3979 2308
Size: 915 494 262 3484
Fragments arranged by size:
3484 915 494 262
SnaBI TAC'GTA
Cuts at: 2145 2145
Size: 5155
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5155
SphI G_CATG'C
Cuts at: 1842 2206 2706 1842
Size: 364 500 4291
Fragments arranged by size:
4291 500 364
SspI AAT'ATT
Cuts at: 831 831
Size: 5155
StuI AGG'CCT
Cuts at: 1332 1332
Size: 5155
StyI C'CwwG_G
Cuts at: 611 2120 2287 2641 611
Size: 1509 167 354 3125
Fragments arranged by size:
3125 1509 354 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1844 1892 1987 2736 2869 3328 3539 1844
Size: 48 95 749 133 459 211 3460
Fragments arranged by size:
3460 749 459 211 133 95 48
TatI w'GTAC_w
Cuts at: 911 1249 1948 4670 911
Size: 338 699 2722 1396
Fragments arranged by size:
2722 1396 699 338
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 458 490 4672 4704 458
Size: 32 4182 32 909
Fragments arranged by size:
4182 909 32 32
Tth111I GACn'n_nGTC
Cuts at: 2950 2950
Size: 5155
XbaI T'CTAG_A
Cuts at: 1339 2184 1339
Size: 845 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1088 1490 2648 132
Size: 956 402 1158 2639
Fragments arranged by size:
2639 1158 956 402
XmaI C'CCGG_G
Cuts at: 483 2173 483
Size: 1690 3465
XmnI GAAnn'nnTTC
Cuts at: 1373 1645 1373
Size: 272 4883
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmtI BsgI BsrGI BstXI
ClaI EcoNI EcoRV FseI FspAI HindIII MfeI NcoI
NdeI NheI NotI NsiI PacI PflMI PsiI PspXI
PsrI SanDI SexAI SfiI SrfI SwaI XhoI ZraI