Restriction map for
pF1KSDA0980
(Circular) (Six-base) MAPSORT of: pF1KSDA0980.seq Check: 939 from: 1 to: 7285
pF1KSDA0980 7285 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1290 2321 1290
Size: 1031 6254
AatII G_ACGT'C
Cuts at: 3329 3329
Size: 7285
AccI GT'mk_AC
Cuts at: 170 4321 170
Size: 4151 3134
Acc65I G'GTAC_C
Cuts at: 1451 4299 1451
Size: 2848 4437
AclI AA'CG_TT
Cuts at: 7125 7125
Size: 7285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 897 1152 1627 1702 1732 1798 2500 3416 3913
Size: 255 475 75 30 66 702 916 497
Cuts at: 3913 4051 4093 4135 4141 5108 5540 6289 6557
Size: 138 42 42 6 967 432 749 268
Cuts at: 6557 897
Size: 1625
Fragments arranged by size:
1625 967 916 749 702 497 475 432
268 255 138 75 66 42 42 30
6
AfeI AGC'GCT
Cuts at: 3275 3275
Size: 7285
AflII C'TTAA_G
Cuts at: 235 235
Size: 7285
AflIII A'CryG_T
Cuts at: 501 2653 3348 4635 5741 6718 501
Size: 2152 695 1287 1106 977 1068
Fragments arranged by size:
2152 1287 1106 1068 977 695
AgeI A'CCGG_T
Cuts at: 5673 5673
Size: 7285
AleI CACnn'nnGTG
Cuts at: 2994 2994
Size: 7285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2283 2510 3260 2283
Size: 227 750 6308
Fragments arranged by size:
6308 750 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1747 1779 4084 4116 1747
Size: 32 2305 32 4916
Fragments arranged by size:
4916 2305 32 32
AlwNI CAG_nnn'CTG
Cuts at: 179 219 258 786 1107 1161 1281 1344 2280
Size: 40 39 528 321 54 120 63 936
Cuts at: 2280 2610 3675 3768 4588 6157 179
Size: 330 1065 93 820 1569 1307
Fragments arranged by size:
1569 1307 1065 936 820 528 330 321
120 93 63 54 40 39
ApaI G_GGCC'C
Cuts at: 1212 2585 1212
Size: 1373 5912
ApaLI G'TGCA_C
Cuts at: 6055 6055
Size: 7285
ApoI r'AATT_y
Cuts at: 319 895 4287 6638 6894 319
Size: 576 3392 2351 256 710
Fragments arranged by size:
3392 2351 710 576 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7285
AvaI C'yCGr_G
Cuts at: 823 950 2236 2536 2939 3122 4303 823
Size: 127 1286 300 403 183 1181 3805
Fragments arranged by size:
3805 1286 1181 403 300 183 127
BamHI G'GATC_C
Cuts at: 4308 4308
Size: 7285
BanI G'GyrC_C
Cuts at: 1451 1556 2259 3091 3133 3885 3950 4183 4299
Size: 105 703 832 42 752 65 233 116
Cuts at: 4299 4713 4961 4996 1451
Size: 414 248 35 3740
Fragments arranged by size:
3740 832 752 703 414 248 233 116
105 65 42 35
BanII G_rGCy'C
Cuts at: 682 815 926 1212 1226 1745 1793 1894 2171
Size: 133 111 286 14 519 48 101 277
Cuts at: 2171 2376 2585 2797 2940 3339 3834 4297 5327
Size: 205 209 212 143 399 495 463 1030
Cuts at: 5327 682
Size: 2640
Fragments arranged by size:
2640 1030 519 495 463 399 286 277
212 209 205 143 133 111 101 48
14
BbeI G_GCGC'C
Cuts at: 1560 3137 3954 4187 4717 4965 1560
Size: 1577 817 233 530 248 3880
Fragments arranged by size:
3880 1577 817 530 248 233
BbsI GAAGACnn'nnnn_
Cuts at: 341 386 848 935 3329 341
Size: 45 462 87 2394 4297
Fragments arranged by size:
4297 2394 462 87 45
BbvCI CC'TCA_GC
Cuts at: 1568 2336 3844 1568
Size: 768 1508 5009
Fragments arranged by size:
5009 1508 768
BciVI GTATCCnnnnn_n'
Cuts at: 5181 5944 7285 5181
Size: 763 1341 5181
Fragments arranged by size:
5181 1341 763
BclI T'GATC_A
Cuts at: 1098 4803 1098
Size: 3705 3580
BglI GCCn_nnn'nGGC
Cuts at: 1934 2648 2707 1934
Size: 714 59 6512
Fragments arranged by size:
6512 714 59
BglII A'GATC_T
Cuts at: 1148 4798 6837 1148
Size: 3650 2039 1596
Fragments arranged by size:
3650 2039 1596
BlpI GC'TnA_GC
Cuts at: 552 1282 1465 1835 3922 4395 552
Size: 730 183 370 2087 473 3442
Fragments arranged by size:
3442 2087 730 473 370 183
Bme1580I G_kGCm'C
Cuts at: 1212 2478 2585 2653 3094 3890 4908 5001 6059
Size: 1266 107 68 441 796 1018 93 1058
Cuts at: 6059 1212
Size: 2438
Fragments arranged by size:
2438 1266 1058 1018 796 441 107 93
68
BmgBI CAC'GTC
Cuts at: 1551 1551
Size: 7285
BmrI ACTGGGnnnn_n'
Cuts at: 3610 4495 4576 4911 7057 3610
Size: 885 81 335 2146 3838
Fragments arranged by size:
3838 2146 885 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3123 5650 3123
Size: 2527 4758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 393 583 799 1029 1047 1216 1366 1390
Size: 125 190 216 230 18 169 150 24
Cuts at: 1390 1429 1462 1561 1720 1744 1946 2053 2161
Size: 39 33 99 159 24 202 107 108
Cuts at: 2161 2182 2428 2482 2488 2944 2954 3102 3160
Size: 21 246 54 6 456 10 148 58
Cuts at: 3160 3187 3298 3361 3397 3697 268
Size: 27 111 63 36 300 3856
Fragments arranged by size:
3856 456 300 246 230 216 202 190
169 159 150 148 125 111 108 107
99 63 58 54 39 36 33 27
24 24 21 18 10 6
Bpu10I CC'TnA_GC
Cuts at: 160 470 772 1568 2243 2336 3844 160
Size: 310 302 796 675 93 1508 3601
Fragments arranged by size:
3601 1508 796 675 310 302 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1777 3823 4459 5338 5832 6130 910
Size: 867 2046 636 879 494 298 2065
Fragments arranged by size:
2065 2046 879 867 636 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 980 2029 2384 3256 6989 980
Size: 1049 355 872 3733 1276
Fragments arranged by size:
3733 1276 1049 872 355
BsaAI yAC'GTr
Cuts at: 2656 4128 4275 4636 2656
Size: 1472 147 361 5305
Fragments arranged by size:
5305 1472 361 147
BsaBI GATnn'nnATC
Cuts at: 6 4797 6836 6
Size: 4791 2039 455
Fragments arranged by size:
4791 2039 455
BsaHI Gr'CG_yC
Cuts at: 1557 2972 3134 3326 3951 4184 4714 4962 5666
Size: 1415 162 192 625 233 530 248 704
Cuts at: 5666 7162 1557
Size: 1496 1680
Fragments arranged by size:
1680 1496 1415 704 625 530 248 233
192 162
BsaWI w'CCGG_w
Cuts at: 4472 4993 5673 5947 6094 6505 4472
Size: 521 680 274 147 411 5252
Fragments arranged by size:
5252 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1384 1414 1435 1465 1747 1777 1898 1928 2533
Size: 30 21 30 282 30 121 30 605
Cuts at: 2533 2563 2569 2599 2645 2675 3133 3163 1384
Size: 30 6 30 46 30 458 30 5506
Fragments arranged by size:
5506 605 458 282 121 46 30 30
30 30 30 30 30 30 21 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1009 1585 1771 2179 2425 2554 2557 2560 2749
Size: 576 186 408 246 129 3 3 189
Cuts at: 2749 3652 3694 4039 4183 1009
Size: 903 42 345 144 4111
Fragments arranged by size:
4111 903 576 408 345 246 189 186
144 129 42 3 3
BseYI C'CCAG_C
Cuts at: 224 747 1929 2027 2367 2955 2984 3575 3860
Size: 523 1182 98 340 588 29 591 285
Cuts at: 3860 4708 5466 6045 6541 224
Size: 848 758 579 496 968
Fragments arranged by size:
1182 968 848 758 591 588 579 523
496 340 285 98 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1757 2253 3048 3844 1757
Size: 496 795 796 5198
Fragments arranged by size:
5198 796 795 496
BsiEI CG_ry'CG
Cuts at: 104 3020 4825 4871 6081 104
Size: 2916 1805 46 1210 1308
Fragments arranged by size:
2916 1805 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 607 1045 1226 1745 1793 2171 3075 3539 3834
Size: 438 181 519 48 378 904 464 295
Cuts at: 3834 4155 4297 5075 5265 6059 607
Size: 321 142 778 190 794 1833
Fragments arranged by size:
1833 904 794 778 519 464 438 378
321 295 190 181 142 48
BsmI GAATG_Cn'
Cuts at: 3748 3748
Size: 7285
BsmBI CGTCTCn'nnnn_
Cuts at: 563 866 2963 563
Size: 303 2097 4885
Fragments arranged by size:
4885 2097 303
Bsp1286I G_dGCh'C
Cuts at: 607 682 815 926 1045 1212 1226 1745 1793
Size: 75 133 111 119 167 14 519 48
Cuts at: 1793 1894 2171 2376 2478 2585 2653 2797 2940
Size: 101 277 205 102 107 68 144 143
Cuts at: 2940 3075 3094 3339 3539 3834 3890 4155 4297
Size: 135 19 245 200 295 56 265 142
Cuts at: 4297 4908 5001 5075 5265 5327 6059 607
Size: 611 93 74 190 62 732 1833
Fragments arranged by size:
1833 732 611 519 295 277 265 245
205 200 190 167 144 143 142 135
133 119 111 107 102 101 93 75
74 68 62 56 48 19 14
BspEI T'CCGG_A
Cuts at: 6505 6505
Size: 7285
BspHI T'CATG_A
Cuts at: 918 1226 6461 918
Size: 308 5235 1742
Fragments arranged by size:
5235 1742 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1290 1814 1986 2321 4115 4333 4547 4849 5230
Size: 524 172 335 1794 218 214 302 381
Cuts at: 5230 1290
Size: 3345
Fragments arranged by size:
3345 1794 524 381 335 302 218 214
172
BsrBI CCG'CTC
Cuts at: 50 1001 1761 2383 2691 4005 5575 5629 7115
Size: 951 760 622 308 1314 1570 54 1486
Cuts at: 7115 7280 50
Size: 165 55
Fragments arranged by size:
1570 1486 1314 951 760 622 308 165
55 54
BsrDI GCAATG_nn'
Cuts at: 5195 5195
Size: 7285
BsrFI r'CCGG_y
Cuts at: 464 1871 3015 5281 5462 5673 464
Size: 1407 1144 2266 181 211 2076
Fragments arranged by size:
2266 2076 1407 1144 211 181
BssHII G'CGCG_C
Cuts at: 2508 2508
Size: 7285
BssSI C'ACGA_G
Cuts at: 1030 2173 5554 5914 1030
Size: 1143 3381 360 2401
Fragments arranged by size:
3381 2401 1143 360
BstAPI GCAn_nnn'nTGC
Cuts at: 258 2610 3918 4660 258
Size: 2352 1308 742 2883
Fragments arranged by size:
2883 2352 1308 742
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7285
BstXI CCAn_nnnn'nTGG
Cuts at: 2702 2996 2702
Size: 294 6991
BstYI r'GATC_y
Cuts at: 1 725 1148 4308 4775 4798 5133 5379 6393
Size: 724 423 3160 467 23 335 246 1014
Cuts at: 6393 6479 6491 6837 1
Size: 86 12 346 449
Fragments arranged by size:
3160 1014 724 467 449 423 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2020 3010 3838 2020
Size: 990 828 5467
Fragments arranged by size:
5467 990 828
BtgI C'CryG_G
Cuts at: 125 188 2229 2562 2823 2989 3778 125
Size: 63 2041 333 261 166 789 3632
Fragments arranged by size:
3632 2041 789 333 261 166 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3898 5233 5414 5577 3898
Size: 1335 181 163 5606
Fragments arranged by size:
5606 1335 181 163
BtsI GCAGTG_nn'
Cuts at: 2668 3699 4526 2668
Size: 1031 827 5427
Fragments arranged by size:
5427 1031 827
DraI TTT'AAA
Cuts at: 3497 4282 3497
Size: 785 6500
DraIII CAC_nnn'GTG
Cuts at: 1735 2284 3165 4146 1735
Size: 549 881 981 4874
Fragments arranged by size:
4874 981 881 549
DrdI GACnn_nn'nnGTC
Cuts at: 1243 4989 5849 1243
Size: 3746 860 2679
Fragments arranged by size:
3746 2679 860
EaeI y'GGCC_r
Cuts at: 453 1109 2640 2692 3017 3902 4868 5042 5433
Size: 656 1531 52 325 885 966 174 391
Cuts at: 5433 5460 6807 453
Size: 27 1347 931
Fragments arranged by size:
1531 1347 966 931 885 656 391 325
174 52 27
EagI C'GGCC_G
Cuts at: 3017 4868 3017
Size: 1851 5434
EarI CTCTTCn'nnn_
Cuts at: 131 587 737 916 1063 1354 3059 3248 3995
Size: 456 150 179 147 291 1705 189 747
Cuts at: 3995 4007 5306 5516 131
Size: 12 1299 210 1900
Fragments arranged by size:
1900 1705 1299 747 456 291 210 189
179 150 147 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1507 2356 4033 5491 5803 5949 7095 1507
Size: 849 1677 1458 312 146 1146 1697
Fragments arranged by size:
1697 1677 1458 1146 849 312 146
EcoICRI GAG'CTC
Cuts at: 1224 1743 1791 2169 3832 4295 1224
Size: 519 48 378 1663 463 4214
Fragments arranged by size:
4214 1663 519 463 378 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 393 583 799 897 1029 1047 1152 1216
Size: 125 190 216 98 132 18 105 64
Cuts at: 1216 1366 1390 1429 1462 1561 1627 1702 1720
Size: 150 24 39 33 99 66 75 18
Cuts at: 1720 1732 1744 1798 1946 2053 2161 2182 2428
Size: 12 12 54 148 107 108 21 246
Cuts at: 2428 2482 2488 2500 2944 2954 3102 3160 3187
Size: 54 6 12 444 10 148 58 27
Cuts at: 3187 3298 3361 3397 3416 3697 3913 4051 4093
Size: 111 63 36 19 281 216 138 42
Cuts at: 4093 4135 4141 5108 5540 6289 6557 268
Size: 42 6 967 432 749 268 996
Fragments arranged by size:
996 967 749 444 432 281 268 246
216 216 190 150 148 148 138 132
125 111 108 107 105 99 98 75
66 64 63 58 54 54 42 42
39 36 33 27 24 21 19 18
18 12 12 12 10 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1627 1750 2539 2835 3679 3751 1627
Size: 123 789 296 844 72 5161
Fragments arranged by size:
5161 844 789 296 123 72
EcoO109I rG'GnC_Cy
Cuts at: 987 1104 1209 1341 1695 1803 2024 2199 2425
Size: 117 105 132 354 108 221 175 226
Cuts at: 2425 2582 2607 3185 4422 987
Size: 157 25 578 1237 3850
Fragments arranged by size:
3850 1237 578 354 226 221 175 157
132 117 108 105 25
EcoRI G'AATT_C
Cuts at: 895 4287 895
Size: 3392 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 904 5302 904
Size: 4398 2887
FseI GG_CCGG'CC
Cuts at: 3019 3019
Size: 7285
FspI TGC'GCA
Cuts at: 5064 5064
Size: 7285
HaeII r_GCGC'y
Cuts at: 552 1560 3137 3277 3954 4187 4717 4965 5989
Size: 1008 1577 140 677 233 530 248 1024
Cuts at: 5989 6968 552
Size: 979 869
Fragments arranged by size:
1577 1024 1008 979 869 677 530 248
233 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1384 1416 1898 1930 2569 2601 2827 2859 3556
Size: 32 482 32 639 32 226 32 697
Cuts at: 3556 3588 5125 5155 5157 5187 1384
Size: 32 1537 30 2 30 3482
Fragments arranged by size:
3482 1537 697 639 482 226 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 309 3665 4322 309
Size: 3356 657 3272
Fragments arranged by size:
3356 3272 657
HindIII A'AGCT_T
Cuts at: 238 1654 238
Size: 1416 5869
HpaI GTT'AAC
Cuts at: 309 3665 309
Size: 3356 3929
KasI G'GCGC_C
Cuts at: 1556 3133 3950 4183 4713 4961 1556
Size: 1577 817 233 530 248 3880
Fragments arranged by size:
3880 1577 817 530 248 233
KpnI G_GTAC'C
Cuts at: 1455 4303 1455
Size: 2848 4437
MluI A'CGCG_T
Cuts at: 501 6718 501
Size: 6217 1068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 439 2432 2531 4127 5956 6140 439
Size: 1993 99 1596 1829 184 1584
Fragments arranged by size:
1993 1829 1596 1584 184 99
MscI TGG'CCA
Cuts at: 1111 2642 5044 1111
Size: 1531 2402 3352
Fragments arranged by size:
3352 2402 1531
MslI CAynn'nnrTG
Cuts at: 1231 2994 3658 5272 5399 1231
Size: 1763 664 1614 127 3117
Fragments arranged by size:
3117 1763 1614 664 127
MspA1I CmG'CkG
Cuts at: 179 258 403 648 764 1311 2031 2214 2231
Size: 79 145 245 116 547 720 183 17
Cuts at: 2231 2460 2564 2917 3774 3966 3987 4394 4708
Size: 229 104 353 857 192 21 407 314
Cuts at: 4708 5068 6083 6328 179
Size: 360 1015 245 1136
Fragments arranged by size:
1136 1015 857 720 547 407 360 353
314 245 245 229 192 183 145 116
104 79 21 17
NaeI GCC'GGC
Cuts at: 466 3017 5464 466
Size: 2551 2447 2287
Fragments arranged by size:
2551 2447 2287
NarI GG'CG_CC
Cuts at: 1557 3134 3951 4184 4714 4962 1557
Size: 1577 817 233 530 248 3880
Fragments arranged by size:
3880 1577 817 530 248 233
NcoI C'CATG_G
Cuts at: 125 3778 125
Size: 3653 3632
NgoMIV G'CCGG_C
Cuts at: 464 3015 5462 464
Size: 2551 2447 2287
Fragments arranged by size:
2551 2447 2287
NruI TCG'CGA
Cuts at: 5646 5646
Size: 7285
NspI r_CATG'y
Cuts at: 2853 3352 4336 4836 5745 2853
Size: 499 984 500 909 4393
Fragments arranged by size:
4393 984 909 500 499
PciI A'CATG_T
Cuts at: 3348 5741 3348
Size: 2393 4892
PflMI CCAn_nnn'nTGG
Cuts at: 2719 2719
Size: 7285
PmeI GTTT'AAAC
Cuts at: 4282 4282
Size: 7285
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1747 1779 6424 6456 1747
Size: 32 4645 32 2576
Fragments arranged by size:
4645 2576 32 32
PpuMI rG'GwC_Cy
Cuts at: 987 1104 1341 1695 1803 2024 2425 3185 987
Size: 117 237 354 108 221 401 760 5087
Fragments arranged by size:
5087 760 401 354 237 221 117 108
PspOMI G'GGCC_C
Cuts at: 1208 2581 1208
Size: 1373 5912
PspXI vC'TCGA_Gb
Cuts at: 2536 2536
Size: 7285
PstI C_TGCA'G
Cuts at: 275 653 697 1565 1691 1811 2207 2219 2318
Size: 378 44 868 126 120 396 12 99
Cuts at: 2318 2489 3456 3773 4330 5015 275
Size: 171 967 317 557 685 2545
Fragments arranged by size:
2545 967 868 685 557 396 378 317
171 126 120 99 44 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7285
PvuII CAG'CTG
Cuts at: 179 258 403 648 1311 2031 2214 2460 3774
Size: 79 145 245 663 720 183 246 1314
Cuts at: 3774 3987 4708 5068 179
Size: 213 721 360 2396
Fragments arranged by size:
2396 1314 721 720 663 360 246 245
213 183 145 79
RsrII CG'GwC_CG
Cuts at: 5478 5478
Size: 7285
SacI G_AGCT'C
Cuts at: 1226 1745 1793 2171 3834 4297 1226
Size: 519 48 378 1663 463 4214
Fragments arranged by size:
4214 1663 519 463 378 48
SacII CC_GC'GG
Cuts at: 2232 2565 2232
Size: 333 6952
SalI G'TCGA_C
Cuts at: 4320 4320
Size: 7285
SanDI GG'GwC_CC
Cuts at: 2425 2425
Size: 7285
SapI GCTCTTCn'nnn_
Cuts at: 737 3059 3995 5306 5516 737
Size: 2322 936 1311 210 2506
Fragments arranged by size:
2506 2322 1311 936 210
SbfI CC_TGCA'GG
Cuts at: 1565 1811 2207 4330 1565
Size: 246 396 2123 4520
Fragments arranged by size:
4520 2123 396 246
ScaI AGT'ACT
Cuts at: 3462 6802 3462
Size: 3340 3945
SexAI A'CCwGG_T
Cuts at: 983 3818 4142 983
Size: 2835 324 4126
Fragments arranged by size:
4126 2835 324
SfcI C'TryA_G
Cuts at: 33 171 271 649 693 1561 1624 1687 1807
Size: 138 100 378 44 868 63 63 120
Cuts at: 1807 2203 2215 2314 2485 3049 3452 3769 4326
Size: 396 12 99 171 564 403 317 557
Cuts at: 4326 5011 6006 6197 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 868 685 564 557 403 396
378 317 191 171 138 120 100 99
63 63 44 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2707 2707
Size: 7285
SfoI GGC'GCC
Cuts at: 1558 3135 3952 4185 4715 4963 1558
Size: 1577 817 233 530 248 3880
Fragments arranged by size:
3880 1577 817 530 248 233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7285
SmaI CCC'GGG
Cuts at: 2941 3124 4305 2941
Size: 183 1181 5921
Fragments arranged by size:
5921 1181 183
SmlI C'TyrA_G
Cuts at: 235 823 889 1792 2536 3802 4438 5353 5847
Size: 588 66 903 744 1266 636 915 494
Cuts at: 5847 6109 235
Size: 262 1411
Fragments arranged by size:
1411 1266 915 903 744 636 588 494
262 66
SnaBI TAC'GTA
Cuts at: 4275 4275
Size: 7285
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7285
SphI G_CATG'C
Cuts at: 2853 4336 4836 2853
Size: 1483 500 5302
Fragments arranged by size:
5302 1483 500
SrfI GCCC'GGGC
Cuts at: 3124 3124
Size: 7285
StuI AGG'CCT
Cuts at: 1847 3106 3517 1847
Size: 1259 411 5615
Fragments arranged by size:
5615 1259 411
StyI C'CwwG_G
Cuts at: 125 2039 2399 3778 4417 4771 125
Size: 1914 360 1379 639 354 2639
Fragments arranged by size:
2639 1914 1379 639 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2287 3109 3137 4866 4999 5458 5669 2287
Size: 822 28 1729 133 459 211 3903
Fragments arranged by size:
3903 1729 822 459 211 133 28
TatI w'GTAC_w
Cuts at: 3460 6800 3460
Size: 3340 3945
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1834 1866 2645 2677 3133 3165 6802 6834 1834
Size: 32 779 32 456 32 3637 32 2285
Fragments arranged by size:
3637 2285 779 456 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3328 5080 3328
Size: 1752 5533
XbaI T'CTAG_A
Cuts at: 936 4314 936
Size: 3378 3907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3273 3951 4778 2281
Size: 992 678 827 4788
Fragments arranged by size:
4788 992 827 678
XhoI C'TCGA_G
Cuts at: 823 2536 823
Size: 1713 5572
XmaI C'CCGG_G
Cuts at: 2939 3122 4303 2939
Size: 183 1181 5921
Fragments arranged by size:
5921 1181 183
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 7285
ZraI GAC'GTC
Cuts at: 3327 3327
Size: 7285
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI
BsrGI BstEII BstZ17I ClaI CspCI EcoRV FspAI MfeI
NdeI NheI NotI NsiI PacI PasI PfoI PshAI
PsiI PsrI SgrAI SspI SwaI