Restriction map for
pF1KSDA0952
(Circular) (Six-base) MAPSORT of: pF1KSDA0952.seq Check: 3240 from: 1 to: 4703
pF1KSDA0952 4703 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 621 1180 1741 621
Size: 559 561 3583
Fragments arranged by size:
3583 561 559
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 1189 2528 2960 3707 3975 1025
Size: 164 1339 432 747 268 1753
Fragments arranged by size:
1753 1339 747 432 268 164
AfeI AGC'GCT
Cuts at: 1246 1246
Size: 4703
AflII C'TTAA_G
Cuts at: 1408 1408
Size: 4703
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 4703
AlwNI CAG_nnn'CTG
Cuts at: 758 2008 3575 758
Size: 1250 1567 1886
Fragments arranged by size:
1886 1567 1250
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 1707 4056 4312 1707
Size: 2349 256 2098
Fragments arranged by size:
2349 2098 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 1723 1723
Size: 4703
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 1719 2133 2381 2416 1719
Size: 414 248 35 4006
Fragments arranged by size:
4006 414 248 35
BanII G_rGCy'C
Cuts at: 565 1717 2747 565
Size: 1152 1030 2521
Fragments arranged by size:
2521 1152 1030
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 298 298
Size: 4703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 656 1096 1130 622
Size: 34 440 34 4195
Fragments arranged by size:
4195 440 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2601 3362 4703 2601
Size: 761 1341 2601
Fragments arranged by size:
2601 1341 761
BclI T'GATC_A
Cuts at: 2223 2223
Size: 4703
BglII A'GATC_T
Cuts at: 1026 2218 4255 1026
Size: 1192 2037 1474
Fragments arranged by size:
2037 1474 1192
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 2328 2421 3477 2328
Size: 93 1056 3554
Fragments arranged by size:
3554 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1008 1492 1658 1915 1996 2331 4475 1008
Size: 484 166 257 81 335 2144 1236
Fragments arranged by size:
2144 1236 484 335 257 166 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 4703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 793 1353 333
Size: 460 560 3683
Fragments arranged by size:
3683 560 460
Bpu10I CC'TnA_GC
Cuts at: 1199 1199
Size: 4703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1461 1879 2758 3250 3548 877
Size: 584 418 879 492 298 2032
Fragments arranged by size:
2032 879 584 492 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 769 1150 4407 769
Size: 381 3257 1065
Fragments arranged by size:
3257 1065 381
BsaAI yAC'GTr
Cuts at: 1695 2056 1695
Size: 361 4342
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1892 2413 3091 3365 3512 3923 1892
Size: 521 678 274 147 411 2672
Fragments arranged by size:
2672 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 799 847 1617 799
Size: 48 770 3885
Fragments arranged by size:
3885 770 48
BseYI C'CCAG_C
Cuts at: 848 857 1241 2128 2886 3463 3959 848
Size: 9 384 887 758 577 496 1592
Fragments arranged by size:
1592 887 758 577 496 384 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1522 1112
Size: 410 4293
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 565 809 1627 1717 2495 2685 3477 565
Size: 244 818 90 778 190 792 1791
Fragments arranged by size:
1791 818 792 778 244 190 90
BsmI GAATG_Cn'
Cuts at: 801 966 1018 801
Size: 165 52 4486
Fragments arranged by size:
4486 165 52
BsmBI CGTCTCn'nnnn_
Cuts at: 178 178
Size: 4703
Bsp1286I G_dGCh'C
Cuts at: 565 809 1627 1717 2328 2421 2495 2685 2747
Size: 244 818 90 611 93 74 190 62
Cuts at: 2747 3477 565
Size: 730 1791
Fragments arranged by size:
1791 818 730 611 244 190 93 90
74 62
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 645 1753 1967 2269 2650 645
Size: 1108 214 302 381 2698
Fragments arranged by size:
2698 1108 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1343 1557 2615 1343
Size: 214 1058 3431
Fragments arranged by size:
3431 1058 214
BsrFI r'CCGG_y
Cuts at: 2701 2882 3091 2701
Size: 181 209 4313
Fragments arranged by size:
4313 209 181
BsrGI T'GTAC_A
Cuts at: 489 489
Size: 4703
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 2080 1123
Size: 957 3746
BstBI TT'CG_AA
Cuts at: 110 3064 110
Size: 2954 1749
BstYI r'GATC_y
Cuts at: 1 1026 1667 1728 2195 2218 2553 2799 3811
Size: 1025 641 61 467 23 335 246 1012
Cuts at: 3811 3897 3909 4255 1
Size: 86 12 346 449
Fragments arranged by size:
1025 1012 641 467 449 346 335 246
86 61 23 12
BstZ17I GTA'TAC
Cuts at: 622 1181 622
Size: 559 4144
BtgI C'CryG_G
Cuts at: 125 125
Size: 4703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 472 592 2653 2834 2997 472
Size: 120 2061 181 163 2178
Fragments arranged by size:
2178 2061 181 163 120
BtsI GCAGTG_nn'
Cuts at: 1946 1946
Size: 4703
DraI TTT'AAA
Cuts at: 1702 1702
Size: 4703
DraIII CAC_nnn'GTG
Cuts at: 514 514
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 707 2288 2462 2853 2880 4225 707
Size: 1581 174 391 27 1345 1185
Fragments arranged by size:
1581 1345 1185 391 174 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 563 1715 563
Size: 1152 3551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 793 1025 1189 1353 2528 2960 3707 3975
Size: 460 232 164 164 1175 432 747 268
Cuts at: 3975 333
Size: 1061
Fragments arranged by size:
1175 1061 747 460 432 268 232 164
164
EcoNI CCTnn'n_nnAGG
Cuts at: 829 829
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 1230 1842 1230
Size: 612 4091
EcoRI G'AATT_C
Cuts at: 1707 1707
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4703
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 1248 1293 2137 2385 3407 4386 1248
Size: 45 844 248 1022 979 1565
Fragments arranged by size:
1565 1022 979 844 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1652 1684 2545 2575 2577 2607 1652
Size: 32 861 30 2 30 3748
Fragments arranged by size:
3748 861 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3374 3558 3374
Size: 184 4519
MscI TGG'CCA
Cuts at: 709 2464 709
Size: 1755 2948
MslI CAynn'nnrTG
Cuts at: 542 2692 2819 542
Size: 2150 127 2426
Fragments arranged by size:
2426 2150 127
MspA1I CmG'CkG
Cuts at: 1373 1814 2128 2488 3501 3746 1373
Size: 441 314 360 1013 245 2330
Fragments arranged by size:
2330 1013 441 360 314 245
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 125 125
Size: 4703
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 1756 2256 3163 1756
Size: 500 907 3296
Fragments arranged by size:
3296 907 500
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PflMI CCAn_nnn'nTGG
Cuts at: 514 514
Size: 4703
PfoI T'CCnGG_A
Cuts at: 770 770
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PstI C_TGCA'G
Cuts at: 1188 1386 1750 2435 1188
Size: 198 364 685 3456
Fragments arranged by size:
3456 685 364 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 1373 2128 2488 1373
Size: 755 360 3588
Fragments arranged by size:
3588 755 360
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 565 1717 565
Size: 1152 3551
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 1450 4220 1450
Size: 2770 1933
SexAI A'CCwGG_T
Cuts at: 510 510
Size: 4703
SfcI C'TryA_G
Cuts at: 33 1184 1382 1746 2431 3424 3615 33
Size: 1151 198 364 685 993 191 1121
Fragments arranged by size:
1151 1121 993 685 364 198 191
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 892 1408 1440 1858 2773 3265 3527 892
Size: 516 32 418 915 492 262 2068
Fragments arranged by size:
2068 915 516 492 418 262 32
SnaBI TAC'GTA
Cuts at: 1695 1695
Size: 4703
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
StyI C'CwwG_G
Cuts at: 125 1837 2191 125
Size: 1712 354 2637
Fragments arranged by size:
2637 1712 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2286 2419 2878 3087 2286
Size: 133 459 209 3902
Fragments arranged by size:
3902 459 209 133
TatI w'GTAC_w
Cuts at: 489 731 1448 4218 489
Size: 242 717 2770 974
Fragments arranged by size:
2770 974 717 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4220 4252 4220
Size: 32 4671
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 972 1539 2198 972
Size: 567 659 3477
Fragments arranged by size:
3477 659 567
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
XmnI GAAnn'nnTTC
Cuts at: 938 938
Size: 4703
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI ZraI