Restriction map for
pF1KSDA0939
(Circular) (Six-base) MAPSORT of: pF1KSDA0939.seq Check: 7039 from: 1 to: 4880
pF1KSDA0939 4880 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 1592 1592
Size: 4880
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 866 1003 2705 3137 3884 4152 300
Size: 566 137 1702 432 747 268 1028
Fragments arranged by size:
1702 1028 747 566 432 268 137
AfeI AGC'GCT
Cuts at: 944 944
Size: 4880
AflII C'TTAA_G
Cuts at: 1696 1696
Size: 4880
AflIII A'CryG_T
Cuts at: 497 1204 2232 3336 4313 497
Size: 707 1028 1104 977 1064
Fragments arranged by size:
1104 1064 1028 977 707
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1543 1543
Size: 4880
AlwNI CAG_nnn'CTG
Cuts at: 1863 2185 3752 1863
Size: 322 1567 2991
Fragments arranged by size:
2991 1567 322
ApaI G_GGCC'C
Cuts at: 1834 1834
Size: 4880
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 641 1259 1338 1884 4233 4489 641
Size: 618 79 546 2349 256 1032
Fragments arranged by size:
2349 1032 618 546 256 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 1900 1900
Size: 4880
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 1471 1896 2310 2558 2593 1471
Size: 425 414 248 35 3758
Fragments arranged by size:
3758 425 414 248 35
BanII G_rGCy'C
Cuts at: 1448 1652 1834 1894 2924 1448
Size: 204 182 60 1030 3404
Fragments arranged by size:
3404 1030 204 182 60
BbeI G_GCGC'C
Cuts at: 2314 2562 2314
Size: 248 4632
BbvCI CC'TCA_GC
Cuts at: 1597 1597
Size: 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 207 241 207
Size: 34 4846
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 1031 2400 1031
Size: 1369 3511
BglI GCCn_nnn'nGGC
Cuts at: 1056 1838 1056
Size: 782 4098
BglII A'GATC_T
Cuts at: 2395 4432 2395
Size: 2037 2843
BlpI GC'TnA_GC
Cuts at: 1992 1992
Size: 4880
Bme1580I G_kGCm'C
Cuts at: 1834 2505 2598 3654 1834
Size: 671 93 1056 3060
Fragments arranged by size:
3060 1056 671 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 2092 2173 2508 4652 1141
Size: 951 81 335 2144 1369
Fragments arranged by size:
2144 1369 951 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 1462
Size: 4880
Bpu10I CC'TnA_GC
Cuts at: 1423 1597 1423
Size: 174 4706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1017 2056 2935 3427 3725 1017
Size: 1039 879 492 298 2172
Fragments arranged by size:
2172 1039 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4584 4584
Size: 4880
BsaAI yAC'GTr
Cuts at: 1872 2233 1872
Size: 361 4519
BsaBI GATnn'nnATC
Cuts at: 6 1378 2394 4431 6
Size: 1372 1016 2037 455
Fragments arranged by size:
2037 1372 1016 455
BsaHI Gr'CG_yC
Cuts at: 1559 1589 1718 2311 2559 3261 4757 1559
Size: 30 129 593 248 702 1496 1682
Fragments arranged by size:
1682 1496 702 593 248 129 30
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 212 242 1627 1648 1657 1678 212
Size: 30 1385 21 9 21 3414
Fragments arranged by size:
3414 1385 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 301 499 1672 1675 1831 163
Size: 138 198 1173 3 156 3212
Fragments arranged by size:
3212 1173 198 156 138 3
BseYI C'CCAG_C
Cuts at: 278 1512 2305 3063 3640 4136 278
Size: 1234 793 758 577 496 1022
Fragments arranged by size:
1234 1022 793 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 1753 280
Size: 1473 3407
BsiEI CG_ry'CG
Cuts at: 104 2422 2468 3676 104
Size: 2318 46 1208 1308
Fragments arranged by size:
2318 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 779 1496 1640 1652 1894 2672 2862 3654
Size: 549 717 144 12 242 778 190 792
Cuts at: 3654 230
Size: 1456
Fragments arranged by size:
1456 792 778 717 549 242 190 144
12
Bsp1286I G_dGCh'C
Cuts at: 230 779 1448 1496 1640 1652 1834 1894 2505
Size: 549 669 48 144 12 182 60 611
Cuts at: 2505 2598 2672 2862 2924 3654 230
Size: 93 74 190 62 730 1456
Fragments arranged by size:
1456 730 669 611 549 190 182 144
93 74 62 60 48 12
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 171 4056 171
Size: 3885 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1775 1930 2144 2446 2827 1775
Size: 155 214 302 381 3828
Fragments arranged by size:
3828 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 1306 3172 3226 4710 4875 50
Size: 1256 1866 54 1484 165 55
Fragments arranged by size:
1866 1484 1256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2792 2792
Size: 4880
BsrFI r'CCGG_y
Cuts at: 2878 3059 3268 2878
Size: 181 209 4490
Fragments arranged by size:
4490 209 181
BssSI C'ACGA_G
Cuts at: 3151 3509 3151
Size: 358 4522
BstAPI GCAn_nnn'nTGC
Cuts at: 1863 2257 1863
Size: 394 4486
BstBI TT'CG_AA
Cuts at: 110 3241 110
Size: 3131 1749
BstYI r'GATC_y
Cuts at: 1 1157 1905 2372 2395 2730 2976 3988 4074
Size: 1156 748 467 23 335 246 1012 86
Cuts at: 4074 4086 4432 1
Size: 12 346 449
Fragments arranged by size:
1156 1012 748 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1184 1448 1773 125
Size: 1059 264 325 3232
Fragments arranged by size:
3232 1059 325 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2830 3011 3174 2830
Size: 181 163 4536
Fragments arranged by size:
4536 181 163
BtsI GCAGTG_nn'
Cuts at: 2123 2123
Size: 4880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 189 224 189
Size: 35 4845
DraI TTT'AAA
Cuts at: 453 1879 453
Size: 1426 3454
DrdI GACnn_nn'nnGTC
Cuts at: 2586 3444 2586
Size: 858 4022
EaeI y'GGCC_r
Cuts at: 734 1091 2465 2639 3030 3057 4402 734
Size: 357 1374 174 391 27 1345 1212
Fragments arranged by size:
1374 1345 1212 391 357 174 27
EagI C'GGCC_G
Cuts at: 2465 2465
Size: 4880
EarI CTCTTCn'nnn_
Cuts at: 140 1501 2903 3113 140
Size: 1361 1402 210 1907
Fragments arranged by size:
1907 1402 1361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 3088 3398 3544 4690 157
Size: 2931 310 146 1146 347
Fragments arranged by size:
2931 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 1650 1892 1650
Size: 242 4638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 866 1003 1462 2705 3137 3884 4152 300
Size: 566 137 459 1243 432 747 268 1028
Fragments arranged by size:
1243 1028 747 566 459 432 268 137
EcoO109I rG'GnC_Cy
Cuts at: 1692 1764 1830 1831 2019 1692
Size: 72 66 1 188 4553
Fragments arranged by size:
4553 188 72 66 1
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 2899 579
Size: 2320 2560
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 946 2314 2562 3584 4563 946
Size: 1368 248 1022 979 1263
Fragments arranged by size:
1368 1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 655 687 805 837 1627 1659 1779
Size: 32 346 32 118 32 790 32 120
Cuts at: 1779 1811 2722 2752 2754 2784 277
Size: 32 911 30 2 30 2373
Fragments arranged by size:
2373 911 790 346 120 118 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1593 1919 1593
Size: 326 4554
KasI G'GCGC_C
Cuts at: 2310 2558 2310
Size: 248 4632
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1613 1625 3551 3735 1613
Size: 12 1926 184 2758
Fragments arranged by size:
2758 1926 184 12
MscI TGG'CCA
Cuts at: 736 1093 2641 736
Size: 357 1548 2975
Fragments arranged by size:
2975 1548 357
MslI CAynn'nnrTG
Cuts at: 349 1114 1786 2869 2996 349
Size: 765 672 1083 127 2233
Fragments arranged by size:
2233 1083 765 672 127
MspA1I CmG'CkG
Cuts at: 299 844 1991 2305 2665 3678 3923 299
Size: 545 1147 314 360 1013 245 1256
Fragments arranged by size:
1256 1147 1013 545 360 314 245
NaeI GCC'GGC
Cuts at: 3061 3061
Size: 4880
NarI GG'CG_CC
Cuts at: 2311 2559 2311
Size: 248 4632
NcoI C'CATG_G
Cuts at: 125 1448 125
Size: 1323 3557
NgoMIV G'CCGG_C
Cuts at: 3059 3059
Size: 4880
NspI r_CATG'y
Cuts at: 501 786 1208 1933 2433 3340 501
Size: 285 422 725 500 907 2041
Fragments arranged by size:
2041 907 725 500 422 285
PciI A'CATG_T
Cuts at: 497 1204 3336 497
Size: 707 2132 2041
Fragments arranged by size:
2132 2041 707
PflMI CCAn_nnn'nTGG
Cuts at: 1808 1808
Size: 4880
PfoI T'CCnGG_A
Cuts at: 1346 1346
Size: 4880
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 139 171 4019 4051 139
Size: 32 3848 32 968
Fragments arranged by size:
3848 968 32 32
PpuMI rG'GwC_Cy
Cuts at: 1692 1764 1692
Size: 72 4808
PsiI TTA'TAA
Cuts at: 436 436
Size: 4880
PspOMI G'GGCC_C
Cuts at: 1830 1830
Size: 4880
PstI C_TGCA'G
Cuts at: 942 1927 2612 942
Size: 985 685 3210
Fragments arranged by size:
3210 985 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 844 2305 2665 844
Size: 1461 360 3059
Fragments arranged by size:
3059 1461 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 1652 1894 1652
Size: 242 4638
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 1501 2903 3113 1501
Size: 1402 210 3268
Fragments arranged by size:
3268 1402 210
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SfcI C'TryA_G
Cuts at: 33 938 1923 2608 3601 3792 33
Size: 905 985 685 993 191 1121
Fragments arranged by size:
1121 993 985 905 685 191
SfoI GGC'GCC
Cuts at: 2312 2560 2312
Size: 248 4632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 1902 1902
Size: 4880
SmlI C'TyrA_G
Cuts at: 996 1696 2035 2950 3442 3704 996
Size: 700 339 915 492 262 2172
Fragments arranged by size:
2172 915 700 492 339 262
SnaBI TAC'GTA
Cuts at: 1872 1872
Size: 4880
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
SspI AAT'ATT
Cuts at: 576 576
Size: 4880
StyI C'CwwG_G
Cuts at: 125 1013 1448 1562 2014 2368 125
Size: 888 435 114 452 354 2637
Fragments arranged by size:
2637 888 452 435 354 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 872 1168 2463 2596 3055 3264 872
Size: 296 1295 133 459 209 2488
Fragments arranged by size:
2488 1295 459 296 209 133
TatI w'GTAC_w
Cuts at: 1290 4395 1290
Size: 3105 1775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4397 4429 4397
Size: 32 4848
Tth111I GACn'n_nGTC
Cuts at: 2677 2677
Size: 4880
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1509 2375 1509
Size: 866 4014
XmaI C'CCGG_G
Cuts at: 1900 1900
Size: 4880
XmnI GAAnn'nnTTC
Cuts at: 485 803 1342 1735 485
Size: 318 539 393 3630
Fragments arranged by size:
3630 539 393 318
ZraI GAC'GTC
Cuts at: 1590 1590
Size: 4880
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AloI AscI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI