Restriction map for
pF1KSDA0932
(Circular) (Six-base) MAPSORT of: pF1KSDA0932.seq Check: 9298 from: 1 to: 6182
pF1KSDA0932 6182 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2061 2384 3052 2061
Size: 323 668 5191
Fragments arranged by size:
5191 668 323
AatII G_ACGT'C
Cuts at: 2141 2141
Size: 6182
AccI GT'mk_AC
Cuts at: 3220 3220
Size: 6182
Acc65I G'GTAC_C
Cuts at: 1235 3198 1235
Size: 1963 4219
AclI AA'CG_TT
Cuts at: 6022 6022
Size: 6182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2089 2283 2824 2831 4007 4439 5186 5454 2089
Size: 194 541 7 1176 432 747 268 2817
Fragments arranged by size:
2817 1176 747 541 432 268 194 7
AflIII A'CryG_T
Cuts at: 909 3534 4638 5615 909
Size: 2625 1104 977 1476
Fragments arranged by size:
2625 1476 1104 977
AgeI A'CCGG_T
Cuts at: 4570 4570
Size: 6182
AleI CACnn'nnGTG
Cuts at: 698 700 1739 698
Size: 2 1039 5141
Fragments arranged by size:
5141 1039 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2269 2269
Size: 6182
AlwNI CAG_nnn'CTG
Cuts at: 270 371 758 2207 3487 5054 270
Size: 101 387 1449 1280 1567 1398
Fragments arranged by size:
1567 1449 1398 1280 387 101
ApaI G_GGCC'C
Cuts at: 153 2307 153
Size: 2154 4028
ApaLI G'TGCA_C
Cuts at: 2453 2776 4952 2453
Size: 323 2176 3683
Fragments arranged by size:
3683 2176 323
ApoI r'AATT_y
Cuts at: 3186 5535 5791 3186
Size: 2349 256 3577
Fragments arranged by size:
3577 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
AvaI C'yCGr_G
Cuts at: 131 202 560 2865 3202 131
Size: 71 358 2305 337 3111
Fragments arranged by size:
3111 2305 358 337 71
BamHI G'GATC_C
Cuts at: 3207 3207
Size: 6182
BanI G'GyrC_C
Cuts at: 190 469 782 1235 3198 3612 3860 3895 190
Size: 279 313 453 1963 414 248 35 2477
Fragments arranged by size:
2477 1963 453 414 313 279 248 35
BanII G_rGCy'C
Cuts at: 153 988 1424 1777 2307 2494 2831 3196 4226
Size: 835 436 353 530 187 337 365 1030
Cuts at: 4226 153
Size: 2109
Fragments arranged by size:
2109 1030 835 530 436 365 353 337
187
BbeI G_GCGC'C
Cuts at: 194 786 3616 3864 194
Size: 592 2830 248 2512
Fragments arranged by size:
2830 2512 592 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1657 1691 1657
Size: 34 6148
BciVI GTATCCnnnnn_n'
Cuts at: 2054 4080 4841 6182 2054
Size: 2026 761 1341 2054
Fragments arranged by size:
2054 2026 1341 761
BclI T'GATC_A
Cuts at: 3702 3702
Size: 6182
BglII A'GATC_T
Cuts at: 3697 5734 3697
Size: 2037 4145
BlpI GC'TnA_GC
Cuts at: 3003 3294 3003
Size: 291 5891
Bme1580I G_kGCm'C
Cuts at: 153 259 1725 2307 2457 2780 3807 3900 4956
Size: 106 1466 582 150 323 1027 93 1056
Cuts at: 4956 153
Size: 1379
Fragments arranged by size:
1466 1379 1056 1027 582 323 150 106
93
BmrI ACTGGGnnnn_n'
Cuts at: 653 693 2838 2895 3394 3475 3810 5954 653
Size: 40 2145 57 499 81 335 2144 881
Fragments arranged by size:
2145 2144 881 499 335 81 57 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 4549 2587
Size: 1962 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 626 1281 1392 1620 292
Size: 334 655 111 228 4854
Fragments arranged by size:
4854 655 334 228 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 1029 1130 3358 4237 4729 5027 567
Size: 462 101 2228 879 492 298 1722
Fragments arranged by size:
2228 1722 879 492 462 298 101
BsaI GGTCTCn'nnnn_
Cuts at: 896 1177 5886 896
Size: 281 4709 1192
Fragments arranged by size:
4709 1192 281
BsaAI yAC'GTr
Cuts at: 1350 1629 3174 3535 1350
Size: 279 1545 361 3997
Fragments arranged by size:
3997 1545 361 279
BsaBI GATnn'nnATC
Cuts at: 6 3696 5733 6
Size: 3690 2037 455
Fragments arranged by size:
3690 2037 455
BsaHI Gr'CG_yC
Cuts at: 191 218 783 1094 2138 2186 2221 3613 3861
Size: 27 565 311 1044 48 35 1392 248
Cuts at: 3861 4563 6059 191
Size: 702 1496 314
Fragments arranged by size:
1496 1392 1044 702 565 314 311 248
48 35 27
BsaWI w'CCGG_w
Cuts at: 1566 2093 2930 3371 3892 4570 4844 4991 5402
Size: 527 837 441 521 678 274 147 411
Cuts at: 5402 1566
Size: 2346
Fragments arranged by size:
2346 837 678 527 521 441 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 540 570 2083 2113 2254 2269 2284 2299 2514
Size: 30 1513 30 141 15 15 15 215
Cuts at: 2514 2544 540
Size: 30 4178
Fragments arranged by size:
4178 1513 215 141 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 623 803 806 1064 2962 2965 265
Size: 358 180 3 258 1898 3 3482
Fragments arranged by size:
3482 1898 358 258 180 3 3
BseYI C'CCAG_C
Cuts at: 855 973 1170 1911 2515 3607 4365 4942 5438
Size: 118 197 741 604 1092 758 577 496
Cuts at: 5438 855
Size: 1599
Fragments arranged by size:
1599 1092 758 741 604 577 496 197
118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 499 1199 2179 2412 2418 2773 2836 3142
Size: 374 700 980 233 6 355 63 306
Cuts at: 3142 125
Size: 3165
Fragments arranged by size:
3165 980 700 374 355 306 233 63
6
BsiEI CG_ry'CG
Cuts at: 104 3021 3724 3770 4978 104
Size: 2917 703 46 1208 1308
Fragments arranged by size:
2917 1308 1208 703 46
BsiHKAI G_wGCw'C
Cuts at: 988 1777 2457 2780 2831 3196 3974 4164 4956
Size: 789 680 323 51 365 778 190 792
Cuts at: 4956 988
Size: 2214
Fragments arranged by size:
2214 792 789 778 680 365 323 190
51
BsiWI C'GTAC_G
Cuts at: 1502 1502
Size: 6182
BsmBI CGTCTCn'nnnn_
Cuts at: 1434 2212 2289 1434
Size: 778 77 5327
Fragments arranged by size:
5327 778 77
Bsp1286I G_dGCh'C
Cuts at: 153 259 988 1424 1725 1777 2307 2457 2494
Size: 106 729 436 301 52 530 150 37
Cuts at: 2494 2780 2831 3196 3807 3900 3974 4164 4226
Size: 286 51 365 611 93 74 190 62
Cuts at: 4226 4956 153
Size: 730 1379
Fragments arranged by size:
1379 730 729 611 530 436 365 301
286 190 150 106 93 74 62 52
51 37
BspEI T'CCGG_A
Cuts at: 1566 2930 5402 1566
Size: 1364 2472 2346
Fragments arranged by size:
2472 2346 1364
BspHI T'CATG_A
Cuts at: 5358 5358
Size: 6182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1385 1522 2061 2192 2384 3052 3232 3446 3748
Size: 137 539 131 192 668 180 214 302
Cuts at: 3748 4129 1385
Size: 381 3438
Fragments arranged by size:
3438 668 539 381 302 214 192 180
137 131
BsrBI CCG'CTC
Cuts at: 50 2086 4474 4528 6012 6177 50
Size: 2036 2388 54 1484 165 55
Fragments arranged by size:
2388 2036 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 317 2139 4094 317
Size: 1822 1955 2405
Fragments arranged by size:
2405 1955 1822
BsrFI r'CCGG_y
Cuts at: 1442 1540 4180 4361 4570 1442
Size: 98 2640 181 209 3054
Fragments arranged by size:
3054 2640 209 181 98
BsrGI T'GTAC_A
Cuts at: 1160 1160
Size: 6182
BssSI C'ACGA_G
Cuts at: 850 2419 4453 4811 850
Size: 1569 2034 358 2221
Fragments arranged by size:
2221 2034 1569 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2207 2789 3559 2207
Size: 582 770 4830
Fragments arranged by size:
4830 770 582
BstBI TT'CG_AA
Cuts at: 110 4543 110
Size: 4433 1749
BstEII G'GTnAC_C
Cuts at: 1272 1272
Size: 6182
BstXI CCAn_nnnn'nTGG
Cuts at: 459 868 1741 2425 459
Size: 409 873 684 4216
Fragments arranged by size:
4216 873 684 409
BstYI r'GATC_y
Cuts at: 1 1265 1412 2096 3207 3674 3697 4032 4278
Size: 1264 147 684 1111 467 23 335 246
Cuts at: 4278 5290 5376 5388 5734 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1264 1111 1012 684 467 449 346 335
246 147 86 23 12
BtgI C'CryG_G
Cuts at: 458 1310 1433 1709 2699 458
Size: 852 123 276 990 3941
Fragments arranged by size:
3941 990 852 276 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 4132 4313 4476 1879
Size: 2253 181 163 3585
Fragments arranged by size:
3585 2253 181 163
BtsI GCAGTG_nn'
Cuts at: 160 1251 2475 2780 3425 160
Size: 1091 1224 305 645 2917
Fragments arranged by size:
2917 1224 1091 645 305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 271 306 271
Size: 35 6147
DraI TTT'AAA
Cuts at: 1323 3181 1323
Size: 1858 4324
DraIII CAC_nnn'GTG
Cuts at: 2565 2789 2565
Size: 224 5958
DrdI GACnn_nn'nnGTC
Cuts at: 910 1093 2281 3888 4746 910
Size: 183 1188 1607 858 2346
Fragments arranged by size:
2346 1607 1188 858 183
EaeI y'GGCC_r
Cuts at: 492 892 1110 1731 1870 1952 2032 3018 3767
Size: 400 218 621 139 82 80 986 749
Cuts at: 3767 3941 4332 4359 5704 492
Size: 174 391 27 1345 970
Fragments arranged by size:
1345 986 970 749 621 400 391 218
174 139 82 80 27
EagI C'GGCC_G
Cuts at: 3018 3767 3018
Size: 749 5433
EarI CTCTTCn'nnn_
Cuts at: 416 564 1709 2550 4205 4415 416
Size: 148 1145 841 1655 210 2183
Fragments arranged by size:
2183 1655 1145 841 210 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4390 4700 4846 5992 4390
Size: 310 146 1146 4580
Fragments arranged by size:
4580 1146 310 146
EcoICRI GAG'CTC
Cuts at: 986 1775 2829 3194 986
Size: 789 1054 365 3974
Fragments arranged by size:
3974 1054 789 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 626 1281 1392 1620 2089 2283 2824 2831
Size: 334 655 111 228 469 194 541 7
Cuts at: 2831 4007 4439 5186 5454 292
Size: 1176 432 747 268 1020
Fragments arranged by size:
1176 1020 747 655 541 469 432 334
268 228 194 111 7
EcoO109I rG'GnC_Cy
Cuts at: 149 150 527 2303 2480 3321 149
Size: 1 377 1776 177 841 3010
Fragments arranged by size:
3010 1776 841 377 177 1
EcoRI G'AATT_C
Cuts at: 3186 3186
Size: 6182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1402 1509 2169 2313 4201 1402
Size: 107 660 144 1888 3383
Fragments arranged by size:
3383 1888 660 144 107
FspI TGC'GCA
Cuts at: 2167 3963 2167
Size: 1796 4386
HaeII r_GCGC'y
Cuts at: 194 213 786 3010 3616 3864 4886 5865 194
Size: 19 573 2224 606 248 1022 979 511
Fragments arranged by size:
2224 1022 979 606 573 511 248 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 540 572 599 631 1539 1571 1753
Size: 32 93 32 27 32 908 32 182
Cuts at: 1753 1785 2254 2286 4024 4054 4056 4086 415
Size: 32 469 32 1738 30 2 30 2511
Fragments arranged by size:
2511 1738 908 469 182 93 32 32
32 32 32 32 30 30 27 2
HincII GTy'rAC
Cuts at: 2370 3221 2370
Size: 851 5331
HindIII A'AGCT_T
Cuts at: 732 1646 732
Size: 914 5268
KasI G'GCGC_C
Cuts at: 190 782 3612 3860 190
Size: 592 2830 248 2512
Fragments arranged by size:
2830 2512 592 248
KpnI G_GTAC'C
Cuts at: 1239 3202 1239
Size: 1963 4219
MluI A'CGCG_T
Cuts at: 5615 5615
Size: 6182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1802 2297 4853 5037 1802
Size: 495 2556 184 2947
Fragments arranged by size:
2947 2556 495 184
MscI TGG'CCA
Cuts at: 1112 1872 3943 1112
Size: 760 2071 3351
Fragments arranged by size:
3351 2071 760
MslI CAynn'nnrTG
Cuts at: 457 698 700 1309 1739 1898 2257 2423 4171
Size: 241 2 609 430 159 359 166 1748
Cuts at: 4171 4298 457
Size: 127 2341
Fragments arranged by size:
2341 1748 609 430 359 241 166 159
127 2
MspA1I CmG'CkG
Cuts at: 180 270 301 1673 2171 2879 3293 3607 3967
Size: 90 31 1372 498 708 414 314 360
Cuts at: 3967 4980 5225 180
Size: 1013 245 1137
Fragments arranged by size:
1372 1137 1013 708 498 414 360 314
245 90 31
NaeI GCC'GGC
Cuts at: 1444 4363 1444
Size: 2919 3263
NarI GG'CG_CC
Cuts at: 191 783 3613 3861 191
Size: 592 2830 248 2512
Fragments arranged by size:
2830 2512 592 248
NcoI C'CATG_G
Cuts at: 458 1310 458
Size: 852 5330
NdeI CA'TA_TG
Cuts at: 1680 1680
Size: 6182
NgoMIV G'CCGG_C
Cuts at: 1442 4361 1442
Size: 2919 3263
NspI r_CATG'y
Cuts at: 538 913 2256 3235 3735 4642 538
Size: 375 1343 979 500 907 2078
Fragments arranged by size:
2078 1343 979 907 500 375
PasI CC'CwG_GG
Cuts at: 523 1279 2026 523
Size: 756 747 4679
Fragments arranged by size:
4679 756 747
PciI A'CATG_T
Cuts at: 909 4638 909
Size: 3729 2453
PflMI CCAn_nnn'nTGG
Cuts at: 371 1108 1177 371
Size: 737 69 5376
Fragments arranged by size:
5376 737 69
PfoI T'CCnGG_A
Cuts at: 1358 1415 1697 3146 1358
Size: 57 282 1449 4394
Fragments arranged by size:
4394 1449 282 57
PmeI GTTT'AAAC
Cuts at: 3181 3181
Size: 6182
PmlI CAC'GTG
Cuts at: 1629 1629
Size: 6182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1027 1059 5321 5353 1027
Size: 32 4262 32 1856
Fragments arranged by size:
4262 1856 32 32
PpuMI rG'GwC_Cy
Cuts at: 527 2480 527
Size: 1953 4229
PspOMI G'GGCC_C
Cuts at: 149 2303 149
Size: 2154 4028
PstI C_TGCA'G
Cuts at: 1193 2556 3063 3229 3914 1193
Size: 1363 507 166 685 3461
Fragments arranged by size:
3461 1363 685 507 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6182
PvuII CAG'CTG
Cuts at: 270 1673 3607 3967 270
Size: 1403 1934 360 2485
Fragments arranged by size:
2485 1934 1403 360
RsrII CG'GwC_CG
Cuts at: 4377 4377
Size: 6182
SacI G_AGCT'C
Cuts at: 988 1777 2831 3196 988
Size: 789 1054 365 3974
Fragments arranged by size:
3974 1054 789 365
SalI G'TCGA_C
Cuts at: 3219 3219
Size: 6182
SanDI GG'GwC_CC
Cuts at: 2480 2480
Size: 6182
SapI GCTCTTCn'nnn_
Cuts at: 416 564 4205 4415 416
Size: 148 3641 210 2183
Fragments arranged by size:
3641 2183 210 148
SbfI CC_TGCA'GG
Cuts at: 1193 3229 1193
Size: 2036 4146
ScaI AGT'ACT
Cuts at: 5699 5699
Size: 6182
SexAI A'CCwGG_T
Cuts at: 1218 1218
Size: 6182
SfcI C'TryA_G
Cuts at: 33 1189 1575 2552 3059 3225 3910 4903 5094
Size: 1156 386 977 507 166 685 993 191
Cuts at: 5094 33
Size: 1121
Fragments arranged by size:
1156 1121 993 977 685 507 386 191
166
SfoI GGC'GCC
Cuts at: 192 784 3614 3862 192
Size: 592 2830 248 2512
Fragments arranged by size:
2830 2512 592 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
SmaI CCC'GGG
Cuts at: 133 562 3204 133
Size: 429 2642 3111
Fragments arranged by size:
3111 2642 429
SmlI C'TyrA_G
Cuts at: 582 1044 1145 3337 4252 4744 5006 582
Size: 462 101 2192 915 492 262 1758
Fragments arranged by size:
2192 1758 915 492 462 262 101
SnaBI TAC'GTA
Cuts at: 3174 3174
Size: 6182
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6182
SphI G_CATG'C
Cuts at: 538 3235 3735 538
Size: 2697 500 2985
Fragments arranged by size:
2985 2697 500
StyI C'CwwG_G
Cuts at: 458 473 1310 1586 2336 3316 3670 458
Size: 15 837 276 750 980 354 2970
Fragments arranged by size:
2970 980 837 750 354 276 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 1440 2018 2877 3765 3898 4357 4566 1260
Size: 180 578 859 888 133 459 209 2876
Fragments arranged by size:
2876 888 859 578 459 209 180 133
TatI w'GTAC_w
Cuts at: 1160 5697 1160
Size: 4537 1645
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 810 842 1056 1088 1208 1240 5699 5731 810
Size: 32 214 32 120 32 4459 32 1261
Fragments arranged by size:
4459 1261 214 120 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3979 3979
Size: 6182
XbaI T'CTAG_A
Cuts at: 3213 3213
Size: 6182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 2845 3677 384
Size: 2461 832 2889
Fragments arranged by size:
2889 2461 832
XmaI C'CCGG_G
Cuts at: 131 560 3202 131
Size: 429 2642 3111
Fragments arranged by size:
3111 2642 429
XmnI GAAnn'nnTTC
Cuts at: 1039 1209 2764 1039
Size: 170 1555 4457
Fragments arranged by size:
4457 1555 170
ZraI GAC'GTC
Cuts at: 2139 2139
Size: 6182
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BbsI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsmI BssHII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SfiI SgrAI SrfI SspI StuI
SwaI XhoI