Restriction map for
pF1KSDA0927
(Circular) (Six-base) MAPSORT of: pF1KSDA0927.seq Check: 2893 from: 1 to: 5986
pF1KSDA0927 5986 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 486 1610 1912 2491 486
Size: 1124 302 579 3981
Fragments arranged by size:
3981 1124 579 302
AatII G_ACGT'C
Cuts at: 2752 2752
Size: 5986
AccI GT'mk_AC
Cuts at: 1067 1232 3022 1067
Size: 165 1790 4031
Fragments arranged by size:
4031 1790 165
Acc65I G'GTAC_C
Cuts at: 3000 3000
Size: 5986
AclI AA'CG_TT
Cuts at: 5826 5826
Size: 5986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 3809 4241 4990 5258 332
Size: 3477 432 749 268 1060
Fragments arranged by size:
3477 1060 749 432 268
AfeI AGC'GCT
Cuts at: 209 209
Size: 5986
AflIII A'CryG_T
Cuts at: 3336 4442 5419 3336
Size: 1106 977 3903
Fragments arranged by size:
3903 1106 977
AgeI A'CCGG_T
Cuts at: 4374 4374
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1358 1358
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1975 1975
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 547 1596 2142 2241 3289 4858 547
Size: 1049 546 99 1048 1569 1675
Fragments arranged by size:
1675 1569 1049 1048 546 99
ApaI G_GGCC'C
Cuts at: 1936 2711 1936
Size: 775 5211
ApaLI G'TGCA_C
Cuts at: 1047 1492 4756 1047
Size: 445 3264 2277
Fragments arranged by size:
3264 2277 445
ApoI r'AATT_y
Cuts at: 2988 5339 5595 2988
Size: 2351 256 3379
Fragments arranged by size:
3379 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5986
AvaI C'yCGr_G
Cuts at: 229 787 1772 2059 2371 3004 229
Size: 558 985 287 312 633 3211
Fragments arranged by size:
3211 985 633 558 312 287
BamHI G'GATC_C
Cuts at: 3009 3009
Size: 5986
BanI G'GyrC_C
Cuts at: 3000 3414 3662 3697 3000
Size: 414 248 35 5289
Fragments arranged by size:
5289 414 248 35
BanII G_rGCy'C
Cuts at: 199 279 1091 1400 1463 1835 1936 1991 2021
Size: 80 812 309 63 372 101 55 30
Cuts at: 2021 2066 2654 2711 2998 4028 199
Size: 45 588 57 287 1030 2157
Fragments arranged by size:
2157 1030 812 588 372 309 287 101
80 63 57 55 45 30
BbeI G_GCGC'C
Cuts at: 3418 3666 3418
Size: 248 5738
BbvCI CC'TCA_GC
Cuts at: 520 2497 520
Size: 1977 4009
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 220 254 2732 2766 220
Size: 34 2478 34 3440
Fragments arranged by size:
3440 2478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2572 3882 4645 5986 2572
Size: 1310 763 1341 2572
Fragments arranged by size:
2572 1341 1310 763
BclI T'GATC_A
Cuts at: 3504 3504
Size: 5986
BfrBI ATG'CAT
Cuts at: 1424 1952 1424
Size: 528 5458
BglI GCCn_nnn'nGGC
Cuts at: 678 817 1388 1777 678
Size: 139 571 389 4887
Fragments arranged by size:
4887 571 389 139
BglII A'GATC_T
Cuts at: 588 3499 5538 588
Size: 2911 2039 1036
Fragments arranged by size:
2911 2039 1036
BlpI GC'TnA_GC
Cuts at: 1297 1470 1726 3096 1297
Size: 173 256 1370 4187
Fragments arranged by size:
4187 1370 256 173
Bme1580I G_kGCm'C
Cuts at: 674 1051 1496 1936 2711 3609 3702 4760 674
Size: 377 445 440 775 898 93 1058 1900
Fragments arranged by size:
1900 1058 898 775 445 440 377 93
BmgBI CAC'GTC
Cuts at: 1760 1760
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 332 887 3196 3277 3612 5758 332
Size: 555 2309 81 335 2146 560
Fragments arranged by size:
2309 2146 560 555 335 81
BmtI G_CTAG'C
Cuts at: 245 245
Size: 5986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 4351 2638
Size: 1713 4273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 990 1063 1075 1384 1467 1565 1945 2523
Size: 758 73 12 309 83 98 380 578
Cuts at: 2523 232
Size: 3695
Fragments arranged by size:
3695 758 578 380 309 98 83 73
12
Bpu10I CC'TnA_GC
Cuts at: 520 2497 520
Size: 1977 4009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 3160 4039 4533 4831 475
Size: 2685 879 494 298 1630
Fragments arranged by size:
2685 1630 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 898 5690 898
Size: 4792 1194
BsaAI yAC'GTr
Cuts at: 2070 2103 2976 3337 2070
Size: 33 873 361 4719
Fragments arranged by size:
4719 873 361 33
BsaBI GATnn'nnATC
Cuts at: 6 3498 5537 6
Size: 3492 2039 455
Fragments arranged by size:
3492 2039 455
BsaHI Gr'CG_yC
Cuts at: 2749 3415 3663 4367 5863 2749
Size: 666 248 704 1496 2872
Fragments arranged by size:
2872 1496 704 666 248
BsaWI w'CCGG_w
Cuts at: 1238 3173 3694 4374 4648 4795 5206 1238
Size: 1935 521 680 274 147 411 2018
Fragments arranged by size:
2018 1935 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 988 1018 1210 1240 1475 1505 988
Size: 30 192 30 235 30 5469
Fragments arranged by size:
5469 235 192 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 583 588 591 670 907 2238 2551 2834
Size: 129 5 3 79 237 1331 313 283
Cuts at: 2834 454
Size: 3606
Fragments arranged by size:
3606 1331 313 283 237 129 79 5
3
BseYI C'CCAG_C
Cuts at: 363 414 566 2032 2814 3409 4167 4746 5242
Size: 51 152 1466 782 595 758 579 496
Cuts at: 5242 363
Size: 1107
Fragments arranged by size:
1466 1107 782 758 595 579 496 152
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1336 1582 1588 2016 383
Size: 953 246 6 428 4353
Fragments arranged by size:
4353 953 428 246 6
BsiEI CG_ry'CG
Cuts at: 104 142 1512 3526 3572 4782 104
Size: 38 1370 2014 46 1210 1308
Fragments arranged by size:
2014 1370 1308 1210 46 38
BsiHKAI G_wGCw'C
Cuts at: 308 1051 1091 1400 1496 2021 2654 2998 3776
Size: 743 40 309 96 525 633 344 778
Cuts at: 3776 3966 4760 308
Size: 190 794 1534
Fragments arranged by size:
1534 794 778 743 633 525 344 309
190 96 40
BsmI GAATG_Cn'
Cuts at: 1954 1954
Size: 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 2740 2740
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 199 279 308 674 1051 1091 1400 1463 1496
Size: 80 29 366 377 40 309 63 33
Cuts at: 1496 1835 1936 1991 2021 2066 2654 2711 2998
Size: 339 101 55 30 45 588 57 287
Cuts at: 2998 3609 3702 3776 3966 4028 4760 199
Size: 611 93 74 190 62 732 1425
Fragments arranged by size:
1425 732 611 588 377 366 339 309
287 190 101 93 80 74 63 62
57 55 45 40 33 30 29
BspEI T'CCGG_A
Cuts at: 1238 5206 1238
Size: 3968 2018
BspHI T'CATG_A
Cuts at: 2324 5162 2324
Size: 2838 3148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 486 1188 1358 1610 1912 2491 2615 3034 3248
Size: 702 170 252 302 579 124 419 214
Cuts at: 3248 3550 3931 486
Size: 302 381 2541
Fragments arranged by size:
2541 702 579 419 381 302 302 252
214 170 124
BsrBI CCG'CTC
Cuts at: 50 4276 4330 5816 5981 50
Size: 4226 54 1486 165 55
Fragments arranged by size:
4226 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3896 3896
Size: 5986
BsrFI r'CCGG_y
Cuts at: 1316 3982 4163 4374 1316
Size: 2666 181 211 2928
Fragments arranged by size:
2928 2666 211 181
BsrGI T'GTAC_A
Cuts at: 2591 2591
Size: 5986
BssSI C'ACGA_G
Cuts at: 4255 4615 4255
Size: 360 5626
BstAPI GCAn_nnn'nTGC
Cuts at: 397 462 3361 397
Size: 65 2899 3022
Fragments arranged by size:
3022 2899 65
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 843 2699 843
Size: 1856 4130
BstYI r'GATC_y
Cuts at: 1 164 588 2096 2120 2375 3009 3476 3499
Size: 163 424 1508 24 255 634 467 23
Cuts at: 3499 3834 4080 5094 5180 5192 5538 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1508 1014 634 467 449 424 346 335
255 246 163 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 270 989 270
Size: 719 5267
BtgI C'CryG_G
Cuts at: 131 149 159 605 842 932 1137 2906 131
Size: 18 10 446 237 90 205 1769 3211
Fragments arranged by size:
3211 1769 446 237 205 90 18 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3934 4115 4278 3934
Size: 181 163 5642
Fragments arranged by size:
5642 181 163
BtsI GCAGTG_nn'
Cuts at: 975 1018 1377 1496 1821 3227 975
Size: 43 359 119 325 1406 3734
Fragments arranged by size:
3734 1406 359 325 119 43
DraI TTT'AAA
Cuts at: 2983 2983
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 1668 3690 4550 1668
Size: 2022 860 3104
Fragments arranged by size:
3104 2022 860
EaeI y'GGCC_r
Cuts at: 139 1169 1509 1914 1937 3569 3743 4134 4161
Size: 1030 340 405 23 1632 174 391 27
Cuts at: 4161 5508 139
Size: 1347 617
Fragments arranged by size:
1632 1347 1030 617 405 391 340 174
27 23
EagI C'GGCC_G
Cuts at: 139 1509 3569 139
Size: 1370 2060 2556
Fragments arranged by size:
2556 2060 1370
EarI CTCTTCn'nnn_
Cuts at: 230 308 1093 1682 2105 4007 4217 230
Size: 78 785 589 423 1902 210 1999
Fragments arranged by size:
1999 1902 785 589 423 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 2854 4192 4504 4650 5796 370
Size: 2484 1338 312 146 1146 560
Fragments arranged by size:
2484 1338 1146 560 312 146
EcoICRI GAG'CTC
Cuts at: 1089 1398 2019 2652 2996 1089
Size: 309 621 633 344 4079
Fragments arranged by size:
4079 633 621 344 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 332 990 1063 1075 1384 1467 1565 1945
Size: 100 658 73 12 309 83 98 380
Cuts at: 1945 2523 3809 4241 4990 5258 232
Size: 578 1286 432 749 268 960
Fragments arranged by size:
1286 960 749 658 578 432 380 309
268 100 98 83 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1189 1481 1927 1189
Size: 292 446 5248
Fragments arranged by size:
5248 446 292
EcoO109I rG'GnC_Cy
Cuts at: 253 563 582 630 819 1265 1719 1932 1965
Size: 310 19 48 189 446 454 213 33
Cuts at: 1965 2493 2708 3123 253
Size: 528 215 415 3116
Fragments arranged by size:
3116 528 454 446 415 310 215 213
189 48 33 19
EcoRI G'AATT_C
Cuts at: 2988 2988
Size: 5986
EcoRV GAT'ATC
Cuts at: 2133 2133
Size: 5986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 691 4003 477
Size: 214 3312 2460
Fragments arranged by size:
3312 2460 214
FspI TGC'GCA
Cuts at: 3765 3765
Size: 5986
HaeII r_GCGC'y
Cuts at: 211 334 1409 3418 3666 4690 5669 211
Size: 123 1075 2009 248 1024 979 528
Fragments arranged by size:
2009 1075 1024 979 528 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 580 612 646 678 1339 1371 1563
Size: 32 272 32 34 32 661 32 192
Cuts at: 1563 1595 1744 1776 2344 2376 2414 2446 2570
Size: 32 149 32 568 32 38 32 124
Cuts at: 2570 2602 3826 3856 3858 3888 276
Size: 32 1224 30 2 30 2374
Fragments arranged by size:
2374 1224 661 568 272 192 149 124
38 34 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3023 3023
Size: 5986
KasI G'GCGC_C
Cuts at: 3414 3662 3414
Size: 248 5738
KpnI G_GTAC'C
Cuts at: 3004 3004
Size: 5986
MluI A'CGCG_T
Cuts at: 5419 5419
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1787 1889 2882 4657 4841 1787
Size: 102 993 1775 184 2932
Fragments arranged by size:
2932 1775 993 184 102
MscI TGG'CCA
Cuts at: 1171 3745 1171
Size: 2574 3412
MslI CAynn'nnrTG
Cuts at: 1342 1358 1826 1957 2629 3973 4100 1342
Size: 16 468 131 672 1344 127 3228
Fragments arranged by size:
3228 1344 672 468 131 127 16
MspA1I CmG'CkG
Cuts at: 133 151 161 429 519 1139 1655 2501 2675
Size: 18 10 268 90 620 516 846 174
Cuts at: 2675 3095 3409 3769 4784 5029 133
Size: 420 314 360 1015 245 1090
Fragments arranged by size:
1090 1015 846 620 516 420 360 314
268 245 174 90 18 10
NaeI GCC'GGC
Cuts at: 1318 4165 1318
Size: 2847 3139
NarI GG'CG_CC
Cuts at: 3415 3663 3415
Size: 248 5738
NcoI C'CATG_G
Cuts at: 842 842
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 1316 4163 1316
Size: 2847 3139
NheI G'CTAG_C
Cuts at: 241 241
Size: 5986
NruI TCG'CGA
Cuts at: 4347 4347
Size: 5986
NsiI A_TGCA'T
Cuts at: 1426 1954 1426
Size: 528 5458
NspI r_CATG'y
Cuts at: 1424 2552 3037 3537 4446 1424
Size: 1128 485 500 909 2964
Fragments arranged by size:
2964 1128 909 500 485
PasI CC'CwG_GG
Cuts at: 579 2560 579
Size: 1981 4005
PciI A'CATG_T
Cuts at: 4442 4442
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1923 2205 1923
Size: 282 5704
PfoI T'CCnGG_A
Cuts at: 1247 1247
Size: 5986
PmeI GTTT'AAAC
Cuts at: 2983 2983
Size: 5986
PmlI CAC'GTG
Cuts at: 2103 2103
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5125 5157 5125
Size: 32 5954
PpuMI rG'GwC_Cy
Cuts at: 253 563 630 1265 1965 2493 253
Size: 310 67 635 700 528 3746
Fragments arranged by size:
3746 700 635 528 310 67
PspOMI G'GGCC_C
Cuts at: 1932 2707 1932
Size: 775 5211
PstI C_TGCA'G
Cuts at: 970 3031 3716 970
Size: 2061 685 3240
Fragments arranged by size:
3240 2061 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5986
PvuII CAG'CTG
Cuts at: 519 2501 3409 3769 519
Size: 1982 908 360 2736
Fragments arranged by size:
2736 1982 908 360
RsrII CG'GwC_CG
Cuts at: 4179 4179
Size: 5986
SacI G_AGCT'C
Cuts at: 1091 1400 2021 2654 2998 1091
Size: 309 621 633 344 4079
Fragments arranged by size:
4079 633 621 344 309
SacII CC_GC'GG
Cuts at: 134 152 162 1140 134
Size: 18 10 978 4980
Fragments arranged by size:
4980 978 18 10
SalI G'TCGA_C
Cuts at: 3021 3021
Size: 5986
SanDI GG'GwC_CC
Cuts at: 563 630 1965 563
Size: 67 1335 4584
Fragments arranged by size:
4584 1335 67
SapI GCTCTTCn'nnn_
Cuts at: 230 1682 4007 4217 230
Size: 1452 2325 210 1999
Fragments arranged by size:
2325 1999 1452 210
SbfI CC_TGCA'GG
Cuts at: 3031 3031
Size: 5986
ScaI AGT'ACT
Cuts at: 5503 5503
Size: 5986
SfcI C'TryA_G
Cuts at: 33 966 1889 2892 3027 3712 4707 4898 33
Size: 933 923 1003 135 685 995 191 1121
Fragments arranged by size:
1121 1003 995 933 923 685 191 135
SfiI GGCCn_nnn'nGGCC
Cuts at: 817 1777 817
Size: 960 5026
SfoI GGC'GCC
Cuts at: 3416 3664 3416
Size: 248 5738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5986
SmaI CCC'GGG
Cuts at: 789 1774 3006 789
Size: 985 1232 3769
Fragments arranged by size:
3769 1232 985
SmlI C'TyrA_G
Cuts at: 490 3139 4054 4548 4810 490
Size: 2649 915 494 262 1666
Fragments arranged by size:
2649 1666 915 494 262
SnaBI TAC'GTA
Cuts at: 2976 2976
Size: 5986
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5986
SphI G_CATG'C
Cuts at: 3037 3537 3037
Size: 500 5486
SrfI GCCC'GGGC
Cuts at: 1774 1774
Size: 5986
StuI AGG'CCT
Cuts at: 1288 1288
Size: 5986
StyI C'CwwG_G
Cuts at: 527 842 1260 2217 3118 3472 527
Size: 315 418 957 901 354 3041
Fragments arranged by size:
3041 957 901 418 354 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2647 3567 3700 4159 4370 2647
Size: 920 133 459 211 4263
Fragments arranged by size:
4263 920 459 211 133
TatI w'GTAC_w
Cuts at: 2243 2591 2879 5501 2243
Size: 348 288 2622 2728
Fragments arranged by size:
2728 2622 348 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1475 1507 5503 5535 1475
Size: 32 3996 32 1926
Fragments arranged by size:
3996 1926 32 32
Tth111I GACn'n_nGTC
Cuts at: 879 2188 3781 879
Size: 1309 1593 3084
Fragments arranged by size:
3084 1593 1309
XbaI T'CTAG_A
Cuts at: 3015 3015
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 849 1140 3479 849
Size: 291 2339 3356
Fragments arranged by size:
3356 2339 291
XmaI C'CCGG_G
Cuts at: 787 1772 3004 787
Size: 985 1232 3769
Fragments arranged by size:
3769 1232 985
ZraI GAC'GTC
Cuts at: 2750 2750
Size: 5986
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbsI BsiWI
BssHII BstEII BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NotI PacI PshAI PsiI
PspXI PsrI SexAI SgrAI SspI SwaI XhoI XmnI